##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062646_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1464397
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.119938104216274	32.0	32.0	32.0	32.0	32.0
2	30.697267885689467	32.0	32.0	32.0	32.0	32.0
3	30.69791183674919	32.0	32.0	32.0	32.0	32.0
4	30.7765879061484	32.0	32.0	32.0	32.0	32.0
5	30.530367106734033	32.0	32.0	32.0	32.0	32.0
6	34.18469718252632	36.0	36.0	36.0	32.0	36.0
7	34.06017971902428	36.0	36.0	36.0	32.0	36.0
8	34.03999598469541	36.0	36.0	36.0	32.0	36.0
9	34.242783207012856	36.0	36.0	36.0	32.0	36.0
10	33.84373704671616	36.0	36.0	36.0	32.0	36.0
11	34.231438605787915	36.0	36.0	36.0	32.0	36.0
12	33.968914850276256	36.0	36.0	36.0	32.0	36.0
13	34.110433850929766	36.0	36.0	36.0	32.0	36.0
14	34.004263188192816	36.0	36.0	36.0	32.0	36.0
15	33.92597704037908	36.0	36.0	36.0	32.0	36.0
16	33.944546458371605	36.0	36.0	36.0	32.0	36.0
17	33.86178269963678	36.0	36.0	36.0	32.0	36.0
18	33.87640168615478	36.0	36.0	36.0	32.0	36.0
19	33.86634635279914	36.0	36.0	36.0	32.0	36.0
20	33.84746759246297	36.0	36.0	36.0	32.0	36.0
21	33.82580201953432	36.0	36.0	36.0	32.0	36.0
22	33.76643833605231	36.0	36.0	36.0	32.0	36.0
23	33.73093498552647	36.0	36.0	36.0	27.0	36.0
24	33.70295486811295	36.0	36.0	36.0	27.0	36.0
25	33.23950813884486	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	12.0
4	57.0
5	138.0
6	246.0
7	65.0
8	229.0
9	259.0
10	166.0
11	49.0
12	110.0
13	71.0
14	275.0
15	341.0
16	517.0
17	709.0
18	988.0
19	1432.0
20	2216.0
21	3212.0
22	4840.0
23	7212.0
24	10115.0
25	14188.0
26	19577.0
27	24946.0
28	33414.0
29	44428.0
30	57469.0
31	77125.0
32	110298.0
33	157519.0
34	336790.0
35	555383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.78758556258746	17.250059585639075	10.865757140544938	26.09659771122853
2	16.69715786519271	20.07515926785613	37.28872126127713	25.938961605674027
3	19.61652269935131	23.77505997594816	27.959342692290097	28.649074632410436
4	12.705002844582012	15.172174046074716	35.712241674048464	36.41058143529481
5	14.607529841886164	36.522547582387105	33.56814278746511	15.301779788261621
6	34.35879567953106	34.99279238657403	16.90140941293818	13.747002520956734
7	30.185826001153472	30.24165443497798	20.836715839421842	18.735803724446704
8	27.88885031043505	33.31490224918461	19.649198828794592	19.14704861158575
9	27.462804093241477	14.23741812184167	18.519176731052763	39.78060105386409
10	15.977887226495948	26.818301150487734	31.68223621780234	25.521575405213976
11	37.45784276659472	20.953332891222203	22.362733039853584	19.226091302329493
12	24.946701510682264	23.51868489823715	28.95369492888293	22.580918662197664
13	30.04156810195124	19.543844775956135	25.098724242134196	25.315862879958434
14	23.24184111174467	20.00891539806842	25.145934947676736	31.603308542510177
15	24.8718364154804	27.929509515791516	22.241568958810593	24.957085109917493
16	25.235264440923093	25.681251717570557	24.69213615873975	24.391347682766597
17	23.73290871910853	26.068651587756836	25.638066510285025	24.560373182849617
18	24.554811942061963	24.7241762208997	27.152437423420267	23.568574413618066
19	25.528564571644242	24.981915098738774	25.329938340400915	24.15958198921607
20	25.545024377136972	24.54012980628876	25.545981549812975	24.36886426676129
21	26.27870977622921	24.486246109582595	24.422590949617383	24.81245316457081
22	25.66025142144345	25.012685963921257	24.995041873939133	24.332020740696162
23	23.935662453535667	24.815783787332162	25.760524393946437	25.48802936518573
24	24.768424658611416	25.177455054178406	25.601665113372256	24.45245517383792
25	24.607578284346	25.012427171985905	25.54882972620564	24.83116481746245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	297.0
1	297.0
2	600.0
3	903.0
4	903.0
5	903.0
6	2193.5
7	3484.0
8	3484.0
9	3484.0
10	3693.5
11	3903.0
12	3903.0
13	3903.0
14	4220.5
15	4538.0
16	4538.0
17	4538.0
18	7148.0
19	9758.0
20	9758.0
21	9758.0
22	16141.0
23	22524.0
24	22524.0
25	22524.0
26	33486.0
27	44448.0
28	44448.0
29	44448.0
30	56909.0
31	69370.0
32	69370.0
33	69370.0
34	82305.5
35	95241.0
36	95241.0
37	95241.0
38	106763.5
39	118286.0
40	118286.0
41	118286.0
42	134568.0
43	150850.0
44	150850.0
45	150850.0
46	171137.5
47	191425.0
48	191425.0
49	191425.0
50	191724.5
51	192024.0
52	192024.0
53	192024.0
54	177972.0
55	163920.0
56	163920.0
57	163920.0
58	150686.0
59	137452.0
60	137452.0
61	137452.0
62	122122.0
63	106792.0
64	106792.0
65	106792.0
66	88536.5
67	70281.0
68	70281.0
69	70281.0
70	53457.0
71	36633.0
72	36633.0
73	36633.0
74	28558.0
75	20483.0
76	20483.0
77	20483.0
78	16537.0
79	12591.0
80	12591.0
81	12591.0
82	8792.5
83	4994.0
84	4994.0
85	4994.0
86	3755.5
87	2517.0
88	2517.0
89	2517.0
90	1677.5
91	838.0
92	838.0
93	838.0
94	513.0
95	188.0
96	188.0
97	188.0
98	422.5
99	657.0
100	657.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008057924183127936
2	0.0027314997230942157
3	0.004097249584641323
4	0.014340373546244633
5	0.03516805893483803
6	0.045547757882596046
7	0.06726318068119506
8	0.0880225785767111
9	0.09560249030829755
10	0.11697647564150979
11	0.11731791310689657
12	0.1283121994923508
13	0.11861537547536631
14	0.12011770032306812
15	0.11048916379916103
16	0.10755280159683474
17	0.11854708798228895
18	0.12762932456157722
19	0.12640014968618482
20	0.11991283784383605
21	0.12489782483848301
22	0.14688639760939143
23	0.13555067375855046
24	0.1307022617500582
25	0.12810733701311872
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1464397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.57732206151066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26062420692584	44.05093238344685
2	12.924684016773208	14.366386522869279
3	3.689774886829158	6.152034216593344
4	1.4463303169520747	3.2153266333028894
5	0.7256963244026292	2.016612917008972
6	0.41690882855304323	1.390240574086777
7	0.276793225240893	1.0768398356560163
8	0.20002821243665603	0.8893625907184245
9	0.13941935318930204	0.6973698864428409
>10	0.7458159267887242	7.7303742337461845
>50	0.07878539904790176	3.015355263942909
>100	0.08384542232460755	9.791221014532388
>500	0.008102131688893361	3.04624420197806
>1k	0.0031917488471398087	2.56169972567516
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3280	0.22398297729372568	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2053	0.14019422328781062	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2043	0.13951134835703705	No Hit
GTACATGGAAGCAGTGGTATCAACG	1902	0.12988281183312994	No Hit
TCCATGTACTCTGCGTTGATACCAC	1655	0.11301580104302318	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1559	0.10646020170759707	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1517	0.10359212699834813	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1482	0.1012020647406407	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.7801245154148773E-4
2	0.0	0.0	0.0	0.0	4.7801245154148773E-4
3	0.0	0.0	0.0	0.0	4.7801245154148773E-4
4	0.0	0.0	0.0	0.0	4.7801245154148773E-4
5	0.0	0.0	0.0	6.828749307735539E-5	4.7801245154148773E-4
6	0.0	0.0	0.0	6.828749307735539E-5	6.828749307735539E-4
7	0.0	0.0	0.0	6.828749307735539E-5	6.828749307735539E-4
8	0.0	0.0	0.0	1.3657498615471077E-4	6.828749307735539E-4
9	0.0	0.0	0.0	1.3657498615471077E-4	6.828749307735539E-4
10	0.0	0.0	0.0	1.3657498615471077E-4	6.828749307735539E-4
11	0.0	0.0	0.0	1.3657498615471077E-4	6.828749307735539E-4
12	0.0	0.0	0.0	2.0486247923206616E-4	8.194499169282646E-4
13	0.0	0.0	0.0	2.0486247923206616E-4	0.0010925998892376862
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	100	1.00462785E-8	13.302179	10
TCGCGTA	100	1.00462785E-8	13.302179	9
ACACCGT	90	5.395741E-7	12.668308	6
GCGTAAC	105	1.9912477E-8	12.667441	11
CGAACGA	85	3.9341394E-6	12.296553	16
CGTTATT	155	1.8189894E-12	12.250015	2
GGTAAAT	70	1.09892644E-4	12.204596	1
ACGCGCG	125	1.4097168E-9	12.160329	13
CGGCTAA	55	0.0030645337	12.09289	9
GTGTTAA	110	3.8549842E-8	12.081317	1
CCTATTC	310	0.0	11.945807	3
GCTCGTA	145	9.640644E-11	11.795036	9
CAAGACG	315	0.0	11.7598095	4
CGCATCG	235	0.0	11.7237215	13
GCGCGCT	130	2.6066118E-9	11.694223	15
CCGCACA	65	8.034927E-4	11.690224	4
CGCCACT	155	2.5465852E-11	11.648265	17
GCGTTAT	180	0.0	11.6019	1
AATCCCG	140	6.7666406E-10	11.538788	19
ATCCCCG	140	6.7848305E-10	11.53642	12
>>END_MODULE