FastQCFastQC Report
Thu 2 Feb 2017
SRR4062646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464397
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15260.10420671443604433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA10900.021.3950612
GTAGGAC10350.021.0440333
TAGGACG9200.019.6092134
TACCGTC3300.019.3338437
GGACCGT1051.7927614E-618.8576416
CGTCGTA3150.018.85699710
ACCGTCG3300.018.6665238
TAGGACA4500.018.5782684
ATACCGT3200.018.562996
CCGTCGT3200.018.5623579
CTGTAGG11900.018.4897791
GTCCTAC8650.018.3144971
GGACGTG9700.018.144816
AGGACGT10000.018.0404765
GTTATAT1355.5197233E-817.9282361
GTCGTAG3500.016.97129611
GTCCTAA6150.016.4574051
TTAGGAC7000.016.3432883
TCCTACA10300.016.2334382
GACGTGG6550.016.1225627
Produced by FastQC (version 0.11.5)