##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062646_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1464397
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.276948122674384	32.0	32.0	32.0	32.0	32.0
2	31.399520075498653	32.0	32.0	32.0	32.0	32.0
3	31.48723194598186	32.0	32.0	32.0	32.0	32.0
4	31.58914556640037	32.0	32.0	32.0	32.0	32.0
5	31.518352605202004	32.0	32.0	32.0	32.0	32.0
6	35.11077528839515	36.0	36.0	36.0	36.0	36.0
7	35.1273834895865	36.0	36.0	36.0	36.0	36.0
8	35.063618677175654	36.0	36.0	36.0	36.0	36.0
9	35.196273961227725	36.0	36.0	36.0	36.0	36.0
10	35.07164792061169	36.0	36.0	36.0	36.0	36.0
11	35.203137537156934	36.0	36.0	36.0	36.0	36.0
12	35.110874988135045	36.0	36.0	36.0	36.0	36.0
13	35.15372539004109	36.0	36.0	36.0	36.0	36.0
14	35.11274538257044	36.0	36.0	36.0	36.0	36.0
15	35.07069462720833	36.0	36.0	36.0	36.0	36.0
16	35.08616857313966	36.0	36.0	36.0	36.0	36.0
17	35.05822191659775	36.0	36.0	36.0	36.0	36.0
18	35.075973933298144	36.0	36.0	36.0	36.0	36.0
19	35.06103194693789	36.0	36.0	36.0	36.0	36.0
20	35.04747141656259	36.0	36.0	36.0	36.0	36.0
21	35.03994886632518	36.0	36.0	36.0	36.0	36.0
22	35.02315355740281	36.0	36.0	36.0	36.0	36.0
23	34.98395585350148	36.0	36.0	36.0	36.0	36.0
24	34.96477457957098	36.0	36.0	36.0	36.0	36.0
25	34.933726305093494	36.0	36.0	36.0	32.0	36.0
26	34.89124328990021	36.0	36.0	36.0	32.0	36.0
27	34.875311817765265	36.0	36.0	36.0	32.0	36.0
28	34.85994440032314	36.0	36.0	36.0	32.0	36.0
29	34.82853215350755	36.0	36.0	36.0	32.0	36.0
30	34.811898685943774	36.0	36.0	36.0	32.0	36.0
31	34.80215064630698	36.0	36.0	36.0	32.0	36.0
32	34.77085243960483	36.0	36.0	36.0	32.0	36.0
33	34.754586358753805	36.0	36.0	36.0	32.0	36.0
34	34.73128939761554	36.0	36.0	36.0	32.0	36.0
35	34.70449201958212	36.0	36.0	36.0	32.0	36.0
36	34.668608307719836	36.0	36.0	36.0	32.0	36.0
37	34.65713669175777	36.0	36.0	36.0	32.0	36.0
38	34.62179381684065	36.0	36.0	36.0	32.0	36.0
39	34.592394002446056	36.0	36.0	36.0	32.0	36.0
40	34.58298535164986	36.0	36.0	36.0	32.0	36.0
41	34.546442665479375	36.0	36.0	36.0	32.0	36.0
42	34.52358684154638	36.0	36.0	36.0	32.0	36.0
43	34.52451896582689	36.0	36.0	36.0	32.0	36.0
44	34.472645054585605	36.0	36.0	36.0	32.0	36.0
45	34.43585585056511	36.0	36.0	36.0	32.0	36.0
46	34.419862919686395	36.0	36.0	36.0	32.0	36.0
47	34.39470307573698	36.0	36.0	36.0	32.0	36.0
48	34.35388627537478	36.0	36.0	36.0	32.0	36.0
49	34.32524445215334	36.0	36.0	36.0	32.0	36.0
50	33.81099933965994	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	18.0
21	41.0
22	108.0
23	358.0
24	1006.0
25	2209.0
26	4723.0
27	8948.0
28	15292.0
29	23496.0
30	33975.0
31	48898.0
32	72182.0
33	117140.0
34	255928.0
35	880071.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19035403506543	17.8041316043074	11.448752439083526	26.556761921543636
2	16.171414093886856	20.41469962174008	37.04147337668856	26.3724129076845
3	18.916912730779	24.069577188559315	28.45864620578699	28.55486387487469
4	12.490832385273363	15.63459014445566	35.93506938706386	35.939508083207116
5	14.33525198426383	36.72303343970248	33.53148087574613	15.41023370028756
6	34.54706241869454	35.31380067400308	16.57269092485924	13.566445982443142
7	30.09051519569515	30.32999976099345	20.869574124467782	18.709910918843615
8	27.9526658412985	33.45779867071566	19.471495776077116	19.118039711908725
9	27.568070680286834	13.907294265148042	18.54859030713666	39.97604474742846
10	15.894596888685243	26.919954083489657	31.703424686065322	25.482024341759786
11	37.71327037681721	20.745535534421336	22.338682747916035	19.202511340845412
12	24.960307894648786	23.408269751986655	28.943039353399385	22.688382999965174
13	30.111711509925243	19.399725620852813	25.162848599116224	25.325714270105713
14	23.200470910552262	19.90648710697987	25.13198265224526	31.76105933022261
15	25.024907863099966	27.84012805270702	22.22730584670687	24.907658237486146
16	25.30092591011864	25.594766992830497	24.67336384873774	24.430943248313127
17	23.922679437338374	25.944398957386554	25.553111622053308	24.57980998322176
18	24.697332758807892	24.507766678025153	27.178080807322058	23.616819755844897
19	25.662508185963233	24.8251669458487	25.430330709500225	24.08199415868784
20	25.74028764057834	24.33431644560867	25.593742680434335	24.331653233378653
21	26.38867738734783	24.244313529732718	24.558640860367785	24.80836822255167
22	25.664283660783244	24.919813411253916	25.0709336334341	24.344969294528738
23	24.0576838111523	24.562738109952424	25.81745250775575	25.56212557113952
24	24.91469184927311	24.93907048430173	25.691803520493416	24.45443414593174
25	24.78228240019612	24.720960231412654	25.7042318442335	24.792525524157725
26	24.052357386692268	25.524840599919287	26.19972589400279	24.22307611938566
27	24.975057993153495	25.111223254349742	25.315334571157962	24.598384181338805
28	24.087593733120187	25.026205325468435	25.790137510524808	25.096063430886566
29	24.31444478512316	24.98250132989893	25.625018352263762	25.07803553271415
30	24.070112134892383	25.14645960077766	26.144549599596285	24.638878664733678
31	24.86887094141821	24.790476899365405	25.228950892415103	25.111701266801283
32	24.423021899116154	25.17561836032169	25.282351712001592	25.119008028560565
33	23.9792897690995	24.786447937273838	26.042323222459483	25.191939071167173
34	24.706619857866414	25.044711236092397	26.12898005117465	24.119688854866542
35	25.17459404792553	25.002031552919053	25.863136840624502	23.960237558530917
36	24.02702272676057	25.44897319511034	25.68483819619953	24.83916588192956
37	24.96310768186496	25.18080820979557	25.18586148428329	24.67022262405618
38	24.36531896746579	25.10944777952973	25.546009722773267	24.979223530231216
39	24.993154178818997	24.566835359537066	25.42964783456945	25.01036262707449
40	25.446173407894168	24.7012251459133	25.799970909527946	24.05263053666458
41	24.30119701146615	24.877748315518264	26.362728139978437	24.458326533037148
42	25.327831182391115	25.481409754322087	25.698768844787306	23.49199021849949
43	24.247932766865816	24.683265535233957	26.073530606795835	24.995271091104392
44	23.77033004028279	25.31458340873411	25.7738850871724	25.141201463810702
45	24.01568700290973	25.438320346190274	25.720347692599756	24.82564495830024
46	23.862466163524072	24.694822985039565	25.98258940887574	25.460121442560617
47	24.480946410670338	24.64790944525934	26.107145881305332	24.76399826276499
48	24.59927191874881	25.5513361472333	25.223214742996607	24.626177191021288
49	24.06581002282851	25.286790399051622	25.614365503343695	25.03303407477617
50	23.78303151399518	26.152607523779416	25.13683106425375	24.927529897971656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	61.0
1	55.0
2	49.0
3	194.5
4	340.0
5	373.5
6	407.0
7	336.5
8	266.0
9	381.5
10	497.0
11	729.0
12	961.0
13	1630.0
14	2299.0
15	3018.5
16	3738.0
17	4206.5
18	4675.0
19	4911.5
20	5148.0
21	5833.5
22	6519.0
23	7234.5
24	7950.0
25	9106.0
26	10262.0
27	12929.0
28	15596.0
29	18432.5
30	21269.0
31	24399.0
32	27529.0
33	32625.5
34	37722.0
35	43752.0
36	49782.0
37	55453.5
38	61125.0
39	63396.0
40	65667.0
41	69614.5
42	73562.0
43	75025.0
44	76488.0
45	84183.0
46	91878.0
47	97826.5
48	103775.0
49	107045.5
50	110316.0
51	105221.5
52	100127.0
53	98758.5
54	97390.0
55	98284.5
56	99179.0
57	96830.0
58	94481.0
59	86828.5
60	79176.0
61	69579.5
62	59983.0
63	52576.5
64	45170.0
65	38687.0
66	32204.0
67	28437.0
68	24670.0
69	22390.5
70	20111.0
71	15804.0
72	11497.0
73	10280.0
74	9063.0
75	6626.5
76	4190.0
77	3819.5
78	3449.0
79	2947.0
80	2445.0
81	1901.0
82	1357.0
83	1171.5
84	986.0
85	814.0
86	642.0
87	460.5
88	279.0
89	176.0
90	73.0
91	47.0
92	21.0
93	16.0
94	11.0
95	7.5
96	4.0
97	5.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015023248477018185
2	0.004097249584641323
3	6.145874376961985E-4
4	2.0486247923206616E-4
5	0.0
6	8.194499169282646E-4
7	1.3657498615471077E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	6.828749307735539E-5
47	6.828749307735539E-5
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1464397.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.18043634252017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.803808567765	47.22824212835282
2	12.825458447202095	15.180324543965623
3	3.5424489632952776	6.289310261067682
4	1.3868574542992032	3.2829931716121425
5	0.6904545690576308	2.0430701335758656
6	0.3982799415042212	1.414222843481593
7	0.2438767617751656	1.0102913222958598
8	0.16933308685489928	0.8016964773839045
9	0.13841764925627129	0.7372455192442842
>10	0.6597906613328128	7.169011735297967
>50	0.06628797100286306	2.758876826919996
>100	0.06688567735645752	8.301340407670954
>500	0.006595917285626418	2.682521225024463
>1k	0.0015043320125112884	1.1008534041068199
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1526	0.10420671443604433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3657498615471077E-4
2	0.0	0.0	0.0	0.0	1.3657498615471077E-4
3	0.0	0.0	0.0	0.0	1.3657498615471077E-4
4	0.0	0.0	0.0	0.0	1.3657498615471077E-4
5	0.0	0.0	0.0	6.828749307735539E-5	2.7314997230942154E-4
6	0.0	0.0	0.0	6.828749307735539E-5	2.7314997230942154E-4
7	0.0	0.0	0.0	1.3657498615471077E-4	2.7314997230942154E-4
8	0.0	0.0	0.0	3.4143746538677696E-4	2.7314997230942154E-4
9	0.0	0.0	0.0	3.4143746538677696E-4	2.7314997230942154E-4
10	0.0	0.0	0.0	3.4143746538677696E-4	2.7314997230942154E-4
11	0.0	0.0	0.0	3.4143746538677696E-4	2.7314997230942154E-4
12	0.0	0.0	0.0	3.4143746538677696E-4	4.7801245154148773E-4
13	0.0	0.0	0.0	4.097249584641323E-4	6.145874376961985E-4
14	0.0	0.0	0.0	4.097249584641323E-4	6.145874376961985E-4
15	0.0	0.0	0.0	4.097249584641323E-4	7.511624238509093E-4
16	0.0	0.0	0.0	4.097249584641323E-4	9.560249030829755E-4
17	0.0	0.0	0.0	4.097249584641323E-4	0.0010243123961603308
18	0.0	0.0	0.0	4.097249584641323E-4	0.0010243123961603308
19	0.0	0.0	0.0	5.462999446188431E-4	0.0010243123961603308
20	0.0	0.0	0.0	7.511624238509093E-4	0.0011608873823150416
21	0.0	0.0	0.0	0.0010925998892376862	0.0012974623684697524
22	0.0	0.0	0.0	0.0015023248477018187	0.0013657498615471078
23	0.0	0.0	0.0	0.002458349750784794	0.0013657498615471078
24	0.0	0.0	0.0	0.004165537077718679	0.0014340373546244632
25	0.0	0.0	0.0	0.005394711953111076	0.0014340373546244632
26	0.0	0.0	0.0	0.006692174321580828	0.0015023248477018187
27	0.0	0.0	0.0	0.008945661593133557	0.0015706123407791739
28	0.0	0.0	0.0	0.015159823463172897	0.0016388998338565293
29	0.0	0.0	0.0	0.02827102213402513	0.0016388998338565293
30	0.0	0.0	0.0	0.05251308217648629	0.0016388998338565293
31	0.0	0.0	0.0	0.10809910154145358	0.0017071873269338847
32	0.0	0.0	0.0	0.1746794072918751	0.0018437623130885955
33	0.0	0.0	0.0	0.2438546377792361	0.0018437623130885955
34	0.0	0.0	0.0	0.31589794297584606	0.002116912285398017
35	0.0	0.0	0.0	0.3971600597378989	0.002116912285398017
36	0.0	0.0	0.0	0.5194629598394425	0.002116912285398017
37	0.0	0.0	0.0	0.6880647802474329	0.002116912285398017
38	0.0	0.0	0.0	0.9143012448127114	0.002116912285398017
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	1090	0.0	21.395061	2
GTAGGAC	1035	0.0	21.044033	3
TAGGACG	920	0.0	19.609213	4
TACCGTC	330	0.0	19.333843	7
GGACCGT	105	1.7927614E-6	18.857641	6
CGTCGTA	315	0.0	18.856997	10
ACCGTCG	330	0.0	18.666523	8
TAGGACA	450	0.0	18.578268	4
ATACCGT	320	0.0	18.56299	6
CCGTCGT	320	0.0	18.562357	9
CTGTAGG	1190	0.0	18.489779	1
GTCCTAC	865	0.0	18.314497	1
GGACGTG	970	0.0	18.14481	6
AGGACGT	1000	0.0	18.040476	5
GTTATAT	135	5.5197233E-8	17.928236	1
GTCGTAG	350	0.0	16.971296	11
GTCCTAA	615	0.0	16.457405	1
TTAGGAC	700	0.0	16.343288	3
TCCTACA	1030	0.0	16.233438	2
GACGTGG	655	0.0	16.122562	7
>>END_MODULE