FastQCFastQC Report
Thu 2 Feb 2017
SRR4062645_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062645_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3449
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG310.8988112496375761No Hit
TATCAACGCAGAGTACTTTTTTTTT170.49289649173673533No Hit
TCCATGTACTCTGCGTTGATACCAC150.43490866917947235No Hit
GTATCAACGCAGAGTACTTTTTTTT110.3189330240649464No Hit
GTACTTTTTTTTTTTTTTTTTTTTT90.2609452015076834No Hit
CATGTACTCTGCGTTGATACCACTG80.2319512902290519No Hit
GCGCAAGACGGACCAGAGCGAAAGC80.2319512902290519No Hit
GTATCTGATCGTCTTCGAACCTCCG70.2029573789504204No Hit
GAATAACGCCGCCGCATCGCCAGTC70.2029573789504204No Hit
GAGTACATGGAAGCAGTGGTATCAA70.2029573789504204No Hit
ACGCAGAGTACATGGAAGCAGTGGT70.2029573789504204No Hit
GTACATGGGTACCTGGTTGATCCTG70.2029573789504204No Hit
GTCCTATTCCATTATTCCTAGCTGC70.2029573789504204No Hit
GAACTACGACGGTATCTGATCGTCT60.17396346767178894No Hit
CATGGAAGCAGTGGTATCAACGCAG60.17396346767178894No Hit
TGCCAGAGTCTCGTTCGTTATCGGA60.17396346767178894No Hit
CTTTAATATACGCTATTGGAGCTGG60.17396346767178894No Hit
ATACAGGACTCTTTCGAGGCCCTGT50.14496955639315745No Hit
CTATTGGAGCTGGAATTACCGCGGC50.14496955639315745No Hit
GATTAAGAGGGACGGCCGGGGGCAT50.14496955639315745No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT50.14496955639315745No Hit
GCTTCCATGTACTCTGCGTTGATAC50.14496955639315745No Hit
CGTTAAAGGATTTAAAGTGGACTCA50.14496955639315745No Hit
GCATATGCTTGTCTCAAAGATTAAG50.14496955639315745No Hit
GAGTACTTTTTTTTTTTTTTTTTTT50.14496955639315745No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG50.14496955639315745No Hit
CTCGGGGGTCGCGTAACTAGTTAGC50.14496955639315745No Hit
GAGCTGGAATTACCGCGGCTGCTGG40.11597564511452595No Hit
CTCCGAGGTCACCCCAACCGAAATT40.11597564511452595No Hit
CATCTAAGGGCATCACAGACCTGTT40.11597564511452595No Hit
GTTCTATTTTGTTGGTTTTCGGAAC40.11597564511452595No Hit
GGACTCTTTCGAGGCCCTGTAATTG40.11597564511452595No Hit
CTCTTAATCATGGCCTCAGTTCCGA40.11597564511452595No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG40.11597564511452595No Hit
ACCATACTCCCCCCGGAACCCAAAG40.11597564511452595No Hit
CCTGGATACCGCAGCTAGGAATAAT40.11597564511452595No Hit
GTCTTGCGCCGGTCCAAGAATTTCA40.11597564511452595No Hit
CTCCGGAATCGAACCCTGATTCCCC40.11597564511452595No Hit
GTCAATCCTGTCCGTGTCCGGGCCG40.11597564511452595No Hit
CCAGAGTCTCGTTCGTTATCGGAAT40.11597564511452595No Hit
GTCCAAGAATTTCACCTCTAGCGGC40.11597564511452595No Hit
GCGTTGATACCACTGCTTCCATGTA40.11597564511452595No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)