##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062645_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3449
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.88895331980284	32.0	32.0	32.0	21.0	32.0
2	29.684836184401277	32.0	32.0	32.0	14.0	32.0
3	30.716439547694986	32.0	32.0	32.0	32.0	32.0
4	31.144389678167585	32.0	32.0	32.0	32.0	32.0
5	30.787184691214843	32.0	32.0	32.0	32.0	32.0
6	34.47810959698463	36.0	36.0	36.0	32.0	36.0
7	34.37141200347927	36.0	36.0	36.0	32.0	36.0
8	34.51377210785735	36.0	36.0	36.0	32.0	36.0
9	34.692084662220935	36.0	36.0	36.0	32.0	36.0
10	34.37402145549434	36.0	36.0	36.0	32.0	36.0
11	34.801391707741374	36.0	36.0	36.0	32.0	36.0
12	34.402725427660194	36.0	36.0	36.0	32.0	36.0
13	34.76253986662801	36.0	36.0	36.0	32.0	36.0
14	34.648883734415776	36.0	36.0	36.0	32.0	36.0
15	34.64221513482169	36.0	36.0	36.0	32.0	36.0
16	34.597274572339806	36.0	36.0	36.0	32.0	36.0
17	34.53029863728617	36.0	36.0	36.0	32.0	36.0
18	34.52391997680487	36.0	36.0	36.0	32.0	36.0
19	34.61119164975355	36.0	36.0	36.0	32.0	36.0
20	34.54537547115106	36.0	36.0	36.0	32.0	36.0
21	34.65758190779936	36.0	36.0	36.0	32.0	36.0
22	34.53957668889533	36.0	36.0	36.0	32.0	36.0
23	34.5401565671209	36.0	36.0	36.0	32.0	36.0
24	34.454334589736156	36.0	36.0	36.0	32.0	36.0
25	34.221513482168746	36.0	36.0	36.0	32.0	36.0
26	34.366772977674685	36.0	36.0	36.0	32.0	36.0
27	34.35024644824587	36.0	36.0	36.0	32.0	36.0
28	34.292838503914176	36.0	36.0	36.0	32.0	36.0
29	34.354305595824876	36.0	36.0	36.0	32.0	36.0
30	34.28124093940273	36.0	36.0	36.0	32.0	36.0
31	34.144389678167585	36.0	36.0	36.0	32.0	36.0
32	34.03914178022615	36.0	36.0	36.0	32.0	36.0
33	34.09828935923456	36.0	36.0	36.0	32.0	36.0
34	34.0742244128733	36.0	36.0	36.0	32.0	36.0
35	33.952739924615834	36.0	36.0	36.0	32.0	36.0
36	34.03624238909829	36.0	36.0	36.0	32.0	36.0
37	33.86488837344158	36.0	36.0	36.0	32.0	36.0
38	33.72078863438678	36.0	36.0	36.0	32.0	36.0
39	33.798782255726294	36.0	36.0	36.0	32.0	36.0
40	33.77993621339519	36.0	36.0	36.0	32.0	36.0
41	33.72629747752972	36.0	36.0	36.0	32.0	36.0
42	33.44592635546535	36.0	36.0	36.0	21.0	36.0
43	33.697013627138304	36.0	36.0	36.0	27.0	36.0
44	33.68918527109307	36.0	36.0	36.0	32.0	36.0
45	33.51087271672949	36.0	36.0	36.0	21.0	36.0
46	33.484778196578716	36.0	36.0	36.0	21.0	36.0
47	33.57610901710641	36.0	36.0	36.0	21.0	36.0
48	33.51957089011307	36.0	36.0	36.0	21.0	36.0
49	33.59379530298637	36.0	36.0	36.0	27.0	36.0
50	32.97796462742824	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	1.0
22	2.0
23	3.0
24	3.0
25	8.0
26	19.0
27	42.0
28	63.0
29	83.0
30	121.0
31	197.0
32	322.0
33	487.0
34	828.0
35	1269.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05653812699333	18.150188460423312	12.525369672368802	24.267903740214557
2	18.93302406494636	20.817628298057407	34.415772687735576	25.833574949260658
3	21.745433458973615	23.60104378080603	29.457813859089594	25.195708901130764
4	15.308785155117425	16.14960858219774	35.57552913888083	32.966077123804
5	19.976804870977094	33.83589446216295	30.211655552334012	15.97564511452595
6	35.40156567120904	35.6335169614381	15.453754711510584	13.511162655842273
7	29.138880835024644	30.06668599594085	19.802841403305305	20.991591765729197
8	27.022325311684547	32.270223253116846	19.30994491156857	21.39750652363004
9	26.645404465062338	14.43896781675848	17.16439547694984	41.75123224122934
10	16.091620759640477	25.543635836474337	30.936503334299797	27.428240069585385
11	37.95302986372862	20.382719628877936	20.585677007828355	21.078573499565092
12	23.717019425920558	23.919976804870977	27.66019135981444	24.702812409394028
13	30.037692084662222	19.28095100028994	24.76080023195129	25.920556683096553
14	24.615830675558133	19.30994491156857	24.673818498115395	31.4004059147579
15	25.543635836474337	26.297477529718762	22.470281240939403	25.688605392867498
16	25.717599304146127	26.732386198898233	23.919976804870977	23.630037692084663
17	23.688025514641925	26.413453174833286	25.166714989852128	24.731806320672657
18	24.992751522180342	24.29689765149319	26.99333140040591	23.717019425920558
19	25.1377210785735	25.688605392867498	25.1377210785735	24.035952449985505
20	25.021745433458975	24.644824586836762	25.775587126703392	24.55784285300087
21	27.13830095679907	25.166714989852128	23.94897071614961	23.746013337199187
22	25.717599304146127	24.96375761090171	24.38387938532908	24.93476369962308
23	23.97796462742824	24.78979414322992	26.297477529718762	24.93476369962308
24	22.44128732966077	26.413453174833286	25.48564801391708	25.65961148158887
25	25.1377210785735	25.1377210785735	24.96375761090171	24.76080023195129
26	24.470861119164976	25.050739344737604	24.064946361264134	26.413453174833286
27	25.601623659031603	25.80458103798202	24.528848941722238	24.064946361264134
28	24.412873296607714	25.253696723688023	25.688605392867498	24.644824586836762
29	25.427660191359813	24.644824586836762	26.47144099739055	23.456074224412873
30	25.514641925195708	25.224702812409394	25.97854450565381	23.282110756741083
31	24.238909828935924	25.514641925195708	25.572629747752973	24.673818498115395
32	24.76080023195129	26.819367932734128	24.180922006378662	24.238909828935924
33	23.94897071614961	25.253696723688023	26.065526239489706	24.731806320672657
34	24.064946361264134	25.891562771817917	26.616410553783705	23.42708031313424
35	24.238909828935924	24.615830675558133	26.181501884604234	24.96375761090171
36	23.311104668019716	26.645404465062338	25.80458103798202	24.238909828935924
37	25.862568860539287	25.195708901130764	24.209915917657295	24.731806320672657
38	23.282110756741083	27.31226442447086	24.673818498115395	24.731806320672657
39	25.195708901130764	25.601623659031603	25.28269063496666	23.919976804870977
40	26.268483618440126	25.398666280081184	23.42708031313424	24.905769788344447
41	23.05015946651203	26.065526239489706	25.427660191359813	25.45665410263845
42	25.166714989852128	26.848361844012757	24.615830675558133	23.36909249057698
43	25.775587126703392	25.630617570310232	24.528848941722238	24.064946361264134
44	23.919976804870977	25.920556683096553	25.398666280081184	24.76080023195129
45	25.427660191359813	24.818788054508552	25.65961148158887	24.093940272542767
46	24.035952449985505	25.688605392867498	25.65961148158887	24.615830675558133
47	23.804001159756453	25.369672368802554	27.51522180342128	23.311104668019716
48	24.412873296607714	27.631197448535804	24.15192809510003	23.804001159756453
49	22.151348216874457	26.007538416932448	26.7033922876196	25.1377210785735
50	25.166714989852128	26.065526239489706	24.93476369962308	23.832995071035082
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	0.5
8	0.0
9	0.5
10	1.0
11	1.5
12	2.0
13	2.0
14	2.0
15	5.0
16	8.0
17	9.5
18	11.0
19	13.5
20	16.0
21	18.5
22	21.0
23	26.5
24	32.0
25	33.0
26	34.0
27	44.0
28	54.0
29	56.5
30	59.0
31	61.0
32	63.0
33	65.5
34	68.0
35	91.0
36	114.0
37	118.0
38	122.0
39	141.5
40	161.0
41	166.0
42	171.0
43	176.0
44	181.0
45	193.5
46	206.0
47	244.5
48	283.0
49	276.5
50	270.0
51	239.5
52	209.0
53	203.5
54	198.0
55	211.0
56	224.0
57	216.5
58	209.0
59	178.5
60	148.0
61	139.0
62	130.0
63	128.0
64	126.0
65	99.5
66	73.0
67	72.5
68	72.0
69	70.0
70	68.0
71	49.0
72	30.0
73	26.0
74	22.0
75	16.0
76	10.0
77	10.5
78	11.0
79	11.0
80	11.0
81	8.0
82	5.0
83	6.0
84	7.0
85	4.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	5.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3449.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.33082052768918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.63492063492063	86.43084952160046
2	3.492063492063492	6.378660481298927
3	0.8888888888888888	2.4354885474050447
4	0.41269841269841273	1.5076833864888375
5	0.25396825396825395	1.1597564511452596
6	0.12698412698412698	0.6958538706871558
7	0.09523809523809523	0.6088721368512612
8	0.031746031746031744	0.2319512902290519
9	0.031746031746031744	0.2609452015076834
>10	0.031746031746031744	0.2899391127863149
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	10	0.2899391127863149	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	9	0.2609452015076834	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	8	0.2319512902290519	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	7	0.2029573789504204	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	7	0.2029573789504204	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	7	0.2029573789504204	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	6	0.17396346767178894	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	6	0.17396346767178894	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	6	0.17396346767178894	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	6	0.17396346767178894	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	5	0.14496955639315745	No Hit
ACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	5	0.14496955639315745	No Hit
GCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT	5	0.14496955639315745	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	5	0.14496955639315745	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA	5	0.14496955639315745	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	5	0.14496955639315745	No Hit
ATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCATTTAT	5	0.14496955639315745	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	5	0.14496955639315745	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	4	0.11597564511452595	No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG	4	0.11597564511452595	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	4	0.11597564511452595	No Hit
ATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACAT	4	0.11597564511452595	No Hit
CAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAA	4	0.11597564511452595	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	4	0.11597564511452595	No Hit
GTATCAACGCAGAGTACATGGGAATAACGCCGCCGCATCGCCAGTCGGCA	4	0.11597564511452595	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	4	0.11597564511452595	No Hit
CCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT	4	0.11597564511452595	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	4	0.11597564511452595	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	4	0.11597564511452595	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG	4	0.11597564511452595	No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT	4	0.11597564511452595	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.028993911278631487	0.0
25	0.0	0.0	0.0	0.028993911278631487	0.0
26	0.0	0.0	0.0	0.028993911278631487	0.0
27	0.0	0.0	0.0	0.028993911278631487	0.0
28	0.0	0.0	0.0	0.028993911278631487	0.0
29	0.0	0.0	0.0	0.08698173383589446	0.0
30	0.0	0.0	0.0	0.11597564511452595	0.0
31	0.0	0.0	0.0	0.11597564511452595	0.0
32	0.0	0.0	0.0	0.11597564511452595	0.0
33	0.0	0.0	0.0	0.11597564511452595	0.0
34	0.0	0.0	0.0	0.2029573789504204	0.0
35	0.0	0.0	0.0	0.2029573789504204	0.0
36	0.0	0.0	0.0	0.2029573789504204	0.0
37	0.0	0.0	0.0	0.31893302406494634	0.0
38	0.0	0.0	0.0	0.3479269353435778	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE