FastQCFastQC Report
Thu 2 Feb 2017
SRR4062644_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062644_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049615
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG117811.1224115509019974No Hit
TCCATGTACTCTGCGTTGATACCAC101960.9714038004411141No Hit
GTATCAACGCAGAGTACTTTTTTTT60990.5810702019311843No Hit
GTACTTTTTTTTTTTTTTTTTTTTT55800.5316235000452547No Hit
GAGTACATGGAAGCAGTGGTATCAA50550.4816051599872334No Hit
TATCAACGCAGAGTACTTTTTTTTT44520.4241555236920204No Hit
CATGTACTCTGCGTTGATACCACTG44360.4226311552331093No Hit
GAGTACTTTTTTTTTTTTTTTTTTT38310.3649909728805324No Hit
CCCATGTACTCTGCGTTGATACCAC33380.31802136974033335No Hit
GTACATGGGAAGCAGTGGTATCAAC30620.29172601382411645No Hit
GCTTCCATGTACTCTGCGTTGATAC30490.2904874644512512No Hit
GCGTTGATACCACTGCTTCCATGTA28470.2712423126574982No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27000.2572371774412523No Hit
CATGGAAGCAGTGGTATCAACGCAG25740.24523277582732717No Hit
GTATCAACGCAGAGTACATGGAAGC23640.22522543980411863No Hit
GTACATGGGGTGGTATCAACGCAAA22730.21655559419406162No Hit
GGTATCAACGCAGAGTACTTTTTTT19410.1849249486716558No Hit
GTATCAACGCAGAGTACATGGGAAG19340.18425803747088218No Hit
ACTCTGCGTTGATACCACTGCTTCC19100.18197148478251549No Hit
ACGCAGAGTACATGGAAGCAGTGGT18010.17158672465618346No Hit
CAGTGGTATCAACGCAGAGTACATG17510.1668230732220862No Hit
TATCAACGCAGAGTACATGGAAGCA14490.13805061856013873No Hit
GTACATGGGTGGTATCAACGCAAAA13820.1316673256384484No Hit
GCAGAGTACTTTTTTTTTTTTTTTT12640.12042510825397883No Hit
TATCAACGCAGAGTACATGGGAAGC11830.11270799293074127No Hit
GCGTTGATACCACTGCTTCCCATGT11650.11099307841446625No Hit
ATACCACTGCTTCCATGTACTCTGC11620.11070725932842043No Hit
GTACATGGGAGTGGTATCAACGCAA11010.10489560457882176No Hit
GAGTACATGGGAAGCAGTGGTATCA10600.10098941040286201No Hit
GTATCAACGCAGAGTACATGGGGTG10590.10089413737418004No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAA359.933987E-519.0004314
CCGATAA402.756553E-416.6277569
CGTAAAT402.7575568E-416.62696316
ACGAACG402.7585606E-416.6261715
CGATAAC508.697321E-515.2025210
CGCATCG1151.8189894E-1214.86990213
GAAATCG456.7536853E-414.77952211
GATAACG456.7536853E-414.77952211
AACGAAC456.7585736E-414.77811114
AATCGTA456.7585736E-414.77811113
TATAACG400.00529351914.2435272
GTCCTAA1203.6379788E-1214.2394521
TAATTCC1051.371518E-913.5697954
CGAACGA500.001497646613.30157116
AAATCGT500.001498185213.30093512
GTTTTCG1001.00462785E-813.30093515
ATCGCCA1301.4551915E-1113.15539816
CGCGCGT1102.73576E-912.9566927
TAATACT1102.746674E-912.9529844
GATATAC2450.012.7864481
Produced by FastQC (version 0.11.5)