##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062644_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1049615
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.843065314424813	32.0	32.0	32.0	32.0	32.0
2	30.72411503265483	32.0	32.0	32.0	32.0	32.0
3	30.741035522548746	32.0	32.0	32.0	32.0	32.0
4	30.680715309899345	32.0	32.0	32.0	32.0	32.0
5	30.68601915940607	32.0	32.0	32.0	32.0	32.0
6	34.21369168695188	36.0	36.0	36.0	32.0	36.0
7	34.01654416143062	36.0	36.0	36.0	32.0	36.0
8	33.98570142385542	36.0	36.0	36.0	32.0	36.0
9	34.03404200587835	36.0	36.0	36.0	32.0	36.0
10	33.88539893198935	36.0	36.0	36.0	32.0	36.0
11	34.16799112055373	36.0	36.0	36.0	32.0	36.0
12	33.99157024242222	36.0	36.0	36.0	32.0	36.0
13	34.079069944694005	36.0	36.0	36.0	32.0	36.0
14	34.01969579321942	36.0	36.0	36.0	32.0	36.0
15	34.032155599910446	36.0	36.0	36.0	32.0	36.0
16	34.00813060026772	36.0	36.0	36.0	32.0	36.0
17	33.92741910128952	36.0	36.0	36.0	32.0	36.0
18	33.97833205508687	36.0	36.0	36.0	32.0	36.0
19	33.94304387799336	36.0	36.0	36.0	32.0	36.0
20	33.866639672641874	36.0	36.0	36.0	32.0	36.0
21	33.788289039314414	36.0	36.0	36.0	32.0	36.0
22	33.78164660375471	36.0	36.0	36.0	32.0	36.0
23	33.7372369868952	36.0	36.0	36.0	27.0	36.0
24	33.75303230232038	36.0	36.0	36.0	32.0	36.0
25	33.20771425713238	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	42.0
5	88.0
6	170.0
7	43.0
8	149.0
9	197.0
10	119.0
11	40.0
12	82.0
13	64.0
14	457.0
15	537.0
16	825.0
17	1210.0
18	1746.0
19	2419.0
20	3415.0
21	4253.0
22	5459.0
23	6956.0
24	8228.0
25	10217.0
26	13080.0
27	15947.0
28	20926.0
29	27413.0
30	35681.0
31	47899.0
32	69212.0
33	100598.0
34	227244.0
35	444896.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.0034043453281	16.8692790467452	11.685393900762334	24.44192270716437
2	15.165590045542027	21.13616875321557	37.608090855389776	26.090150345852624
3	19.60601420947978	26.222178183484644	27.71686987474049	26.454937732295075
4	12.366147742020319	15.958409799919579	34.60072531296511	37.074717145094986
5	13.705588594083562	37.688986733760295	33.07706236657518	15.52836230558097
6	33.409108022299684	33.958688973972585	18.111580374069618	14.520622629658117
7	28.88221969691192	31.194753540730797	21.46041321872831	18.462613543628972
8	28.916842515788275	31.732176622762392	20.527575971111478	18.82340489033785
9	27.677928587299515	15.011772424585438	18.71381612512242	38.59648286299262
10	16.06927903334421	27.534199798168252	31.551090330199028	24.84543083828851
11	36.12399595951503	22.053073811342927	21.366782749532856	20.45614747960919
12	24.169056327306237	24.52736232979034	29.22950854077114	22.07407280213228
13	30.433310377587052	19.972262336488	25.47153074130527	24.122896544619678
14	24.27788286444964	19.429269674657387	25.901972735970453	30.390874724922522
15	25.524611584278347	25.960667245276543	25.258514625795193	23.256206544649924
16	25.079807146638185	26.046649148994007	25.28706251520079	23.58648118916702
17	23.08126685368876	25.63983208074318	27.166882400241903	24.11201866532616
18	24.556540121016017	24.40496007310947	28.539022084516606	22.499477721357906
19	24.165651504903778	27.187955809221453	26.174383962956217	22.472008722918552
20	24.421505064543236	23.775071134440516	28.48105672917976	23.322367071836485
21	25.68005082495154	25.163406798028053	24.822093591172028	24.33444878584838
22	24.075227871447392	25.586864986942654	25.724833002407284	24.613074139202666
23	23.13757777782018	25.302668058287807	25.92527223429608	25.634481929595925
24	23.03172592222303	27.293107987956816	25.827110132528418	23.848055957291734
25	24.701604163804813	25.04435871605843	25.391501442373738	24.862535677763024
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	543.0
1	543.0
2	1100.0
3	1657.0
4	1657.0
5	1657.0
6	4483.5
7	7310.0
8	7310.0
9	7310.0
10	6887.5
11	6465.0
12	6465.0
13	6465.0
14	5126.5
15	3788.0
16	3788.0
17	3788.0
18	6032.0
19	8276.0
20	8276.0
21	8276.0
22	12656.5
23	17037.0
24	17037.0
25	17037.0
26	24887.5
27	32738.0
28	32738.0
29	32738.0
30	40359.0
31	47980.0
32	47980.0
33	47980.0
34	54383.0
35	60786.0
36	60786.0
37	60786.0
38	68147.0
39	75508.0
40	75508.0
41	75508.0
42	91356.0
43	107204.0
44	107204.0
45	107204.0
46	144212.0
47	181220.0
48	181220.0
49	181220.0
50	163241.5
51	145263.0
52	145263.0
53	145263.0
54	126509.5
55	107756.0
56	107756.0
57	107756.0
58	97901.5
59	88047.0
60	88047.0
61	88047.0
62	77533.5
63	67020.0
64	67020.0
65	67020.0
66	55265.0
67	43510.0
68	43510.0
69	43510.0
70	33175.5
71	22841.0
72	22841.0
73	22841.0
74	17459.0
75	12077.0
76	12077.0
77	12077.0
78	9770.0
79	7463.0
80	7463.0
81	7463.0
82	5170.5
83	2878.0
84	2878.0
85	2878.0
86	2066.0
87	1254.0
88	1254.0
89	1254.0
90	843.0
91	432.0
92	432.0
93	432.0
94	258.5
95	85.0
96	85.0
97	85.0
98	281.0
99	477.0
100	477.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007050204122463951
2	0.003334556003868085
3	0.0032392829751861393
4	0.013052404929426505
5	0.03191646460845167
6	0.04306340896423927
7	0.06469038647504084
8	0.08517408764165908
9	0.09317702205094248
10	0.11594727590592742
11	0.11680473316406492
12	0.1285233156919442
13	0.11632836802065519
14	0.12137783854079828
15	0.10718215726718844
16	0.10956398298423708
17	0.1194723779671594
18	0.12614148997489558
19	0.12785640449117058
20	0.11890073979506771
21	0.12490294060203026
22	0.14729210234228743
23	0.13690734221595538
24	0.1321436907818581
25	0.12795167751985254
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1049615.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.25206294269549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73524539757062	34.5665927416623
2	14.519568509701882	13.431199932230218
3	4.801903459163738	6.662938231139652
4	2.066206341600605	3.8226522305723107
5	1.0555339304049578	2.4410310893620433
6	0.6228170808434228	1.7283944894973515
7	0.4093334591639879	1.325276184246283
8	0.27133771256471856	1.0039943168216303
9	0.19910337116695473	0.8288047489785167
>10	1.0897695760300536	9.488570537931848
>50	0.11087763059368265	3.579274642806307
>100	0.10302523929368636	9.274145885283415
>500	0.008877926375293074	2.7938557755938884
>1k	0.005368048505991161	5.356617078858515
>5k	6.193902122297493E-4	1.5979896280817627
>10k+	4.1292680815316617E-4	2.0986624869339607
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	11781	1.1224115509019974	No Hit
TCCATGTACTCTGCGTTGATACCAC	10196	0.9714038004411141	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6099	0.5810702019311843	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5580	0.5316235000452547	No Hit
GAGTACATGGAAGCAGTGGTATCAA	5055	0.4816051599872334	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4452	0.4241555236920204	No Hit
CATGTACTCTGCGTTGATACCACTG	4436	0.4226311552331093	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	3831	0.3649909728805324	No Hit
CCCATGTACTCTGCGTTGATACCAC	3338	0.31802136974033335	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3062	0.29172601382411645	No Hit
GCTTCCATGTACTCTGCGTTGATAC	3049	0.2904874644512512	No Hit
GCGTTGATACCACTGCTTCCATGTA	2847	0.2712423126574982	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2700	0.2572371774412523	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2574	0.24523277582732717	No Hit
GTATCAACGCAGAGTACATGGAAGC	2364	0.22522543980411863	No Hit
GTACATGGGGTGGTATCAACGCAAA	2273	0.21655559419406162	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1941	0.1849249486716558	No Hit
GTATCAACGCAGAGTACATGGGAAG	1934	0.18425803747088218	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1910	0.18197148478251549	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1801	0.17158672465618346	No Hit
CAGTGGTATCAACGCAGAGTACATG	1751	0.1668230732220862	No Hit
TATCAACGCAGAGTACATGGAAGCA	1449	0.13805061856013873	No Hit
GTACATGGGTGGTATCAACGCAAAA	1382	0.1316673256384484	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1264	0.12042510825397883	No Hit
TATCAACGCAGAGTACATGGGAAGC	1183	0.11270799293074127	No Hit
GCGTTGATACCACTGCTTCCCATGT	1165	0.11099307841446625	No Hit
ATACCACTGCTTCCATGTACTCTGC	1162	0.11070725932842043	No Hit
GTACATGGGAGTGGTATCAACGCAA	1101	0.10489560457882176	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1060	0.10098941040286201	No Hit
GTATCAACGCAGAGTACATGGGGTG	1059	0.10089413737418004	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	9.527302868194528E-5	0.0
8	0.0	0.0	0.0	1.9054605736389056E-4	0.0
9	0.0	0.0	0.0	1.9054605736389056E-4	0.0
10	0.0	0.0	0.0	1.9054605736389056E-4	0.0
11	0.0	0.0	0.0	1.9054605736389056E-4	0.0
12	0.0	0.0	0.0	1.9054605736389056E-4	5.716381720916717E-4
13	0.0	0.0	0.0	1.9054605736389056E-4	7.621842294555622E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAA	35	9.933987E-5	19.00043	14
CCGATAA	40	2.756553E-4	16.627756	9
CGTAAAT	40	2.7575568E-4	16.626963	16
ACGAACG	40	2.7585606E-4	16.62617	15
CGATAAC	50	8.697321E-5	15.20252	10
CGCATCG	115	1.8189894E-12	14.869902	13
GAAATCG	45	6.7536853E-4	14.779522	11
GATAACG	45	6.7536853E-4	14.779522	11
AACGAAC	45	6.7585736E-4	14.778111	14
AATCGTA	45	6.7585736E-4	14.778111	13
TATAACG	40	0.005293519	14.243527	2
GTCCTAA	120	3.6379788E-12	14.239452	1
TAATTCC	105	1.371518E-9	13.569795	4
CGAACGA	50	0.0014976466	13.301571	16
AAATCGT	50	0.0014981852	13.300935	12
GTTTTCG	100	1.00462785E-8	13.300935	15
ATCGCCA	130	1.4551915E-11	13.155398	16
CGCGCGT	110	2.73576E-9	12.956692	7
TAATACT	110	2.746674E-9	12.952984	4
GATATAC	245	0.0	12.786448	1
>>END_MODULE