FastQCFastQC Report
Thu 2 Feb 2017
SRR4062644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1049615
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA63680.6066986466466276No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC51360.48932227531047096No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA23800.22674980826302976No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC21780.20750465646927682No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20760.1977868075437184No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16820.16024923424303197No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG16120.1535801222352958No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA15130.14414809239578322No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG14700.14005135216245956No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC14320.13643097707254564No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT13910.1325247828965859No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13430.12795167751985254No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10890.10375232823463841No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10530.10032249920208838No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT300.00574488629.333114
AACGCGC300.00574488629.3331142
TATGCGT703.215021E-521.99983439
GTGTATA4450.021.7588651
TGGTATA3100.021.29016144
GATCGAA654.93182E-420.30753711
AATCGAG1101.2653436E-719.99984722
TGATCGA808.987655E-519.24985510
GTATATC4600.019.130293
CGCAATA2550.018.98024736
TGTATAT4950.018.6674142
ACGACGG2400.018.3331956
ACGGTAT2400.018.3331959
TATATCA4850.018.1441924
CGCTAGA2550.018.1175137
ATAAGGT851.430688E-418.117514
CGACGGT2450.017.959057
TACGACG2450.017.959055
GTCGTAT1355.523907E-817.92578943
CGGTGAT1355.523907E-817.92578943
Produced by FastQC (version 0.11.5)