##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062644_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1049615
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11381601825431	32.0	32.0	32.0	32.0	32.0
2	31.394118795939463	32.0	32.0	32.0	32.0	32.0
3	31.49087141475684	32.0	32.0	32.0	32.0	32.0
4	31.606793919675308	32.0	32.0	32.0	32.0	32.0
5	31.5354801522463	32.0	32.0	32.0	32.0	32.0
6	35.11436479089952	36.0	36.0	36.0	36.0	36.0
7	35.127704920375564	36.0	36.0	36.0	36.0	36.0
8	35.08022465380163	36.0	36.0	36.0	36.0	36.0
9	35.15453952163412	36.0	36.0	36.0	36.0	36.0
10	35.07082120587072	36.0	36.0	36.0	36.0	36.0
11	35.17576730515475	36.0	36.0	36.0	36.0	36.0
12	35.09152784592446	36.0	36.0	36.0	36.0	36.0
13	35.1098269365434	36.0	36.0	36.0	36.0	36.0
14	35.073803251668465	36.0	36.0	36.0	36.0	36.0
15	35.06318888354301	36.0	36.0	36.0	36.0	36.0
16	35.06195319236101	36.0	36.0	36.0	36.0	36.0
17	35.04735450617608	36.0	36.0	36.0	36.0	36.0
18	35.049586753238096	36.0	36.0	36.0	36.0	36.0
19	35.002496153351466	36.0	36.0	36.0	36.0	36.0
20	34.96490141623357	36.0	36.0	36.0	36.0	36.0
21	34.92249348570667	36.0	36.0	36.0	32.0	36.0
22	34.90581213111474	36.0	36.0	36.0	32.0	36.0
23	34.86878331578722	36.0	36.0	36.0	32.0	36.0
24	34.85744106172263	36.0	36.0	36.0	32.0	36.0
25	34.823590554631934	36.0	36.0	36.0	32.0	36.0
26	34.76772340334313	36.0	36.0	36.0	32.0	36.0
27	34.750457072355104	36.0	36.0	36.0	32.0	36.0
28	34.7128842480338	36.0	36.0	36.0	32.0	36.0
29	34.68963000719312	36.0	36.0	36.0	32.0	36.0
30	34.64886172548982	36.0	36.0	36.0	32.0	36.0
31	34.61884881599443	36.0	36.0	36.0	32.0	36.0
32	34.554287048108115	36.0	36.0	36.0	32.0	36.0
33	34.49016544161431	36.0	36.0	36.0	32.0	36.0
34	34.44035289129824	36.0	36.0	36.0	32.0	36.0
35	34.34862783020441	36.0	36.0	36.0	32.0	36.0
36	34.302247967111754	36.0	36.0	36.0	32.0	36.0
37	34.249073231613494	36.0	36.0	36.0	32.0	36.0
38	34.1657017096745	36.0	36.0	36.0	32.0	36.0
39	34.04970774998452	36.0	36.0	36.0	32.0	36.0
40	33.94580107944341	36.0	36.0	36.0	32.0	36.0
41	33.93652529737094	36.0	36.0	36.0	32.0	36.0
42	33.78506023637238	36.0	36.0	36.0	32.0	36.0
43	33.791786512197326	36.0	36.0	36.0	32.0	36.0
44	33.76257770706402	36.0	36.0	36.0	32.0	36.0
45	33.544986495048185	36.0	36.0	36.0	21.0	36.0
46	33.620820967688154	36.0	36.0	36.0	27.0	36.0
47	33.48396602563797	36.0	36.0	36.0	21.0	36.0
48	33.447765132929696	36.0	36.0	36.0	21.0	36.0
49	33.4372184086546	36.0	36.0	36.0	21.0	36.0
50	32.825612248300565	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	5.0
20	8.0
21	31.0
22	116.0
23	389.0
24	1083.0
25	2533.0
26	5285.0
27	10187.0
28	17333.0
29	25308.0
30	34168.0
31	45677.0
32	64853.0
33	89167.0
34	172390.0
35	581081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.60567309927924	17.341468434714	12.23314440789599	24.81971405811077
2	14.792290535049215	21.4581822789779	37.25183813896587	26.497689047007007
3	19.187852273030416	26.293144514353877	28.108291444838663	26.410711767777045
4	12.194506160371642	16.27325144910691	34.68634123847669	36.84590115204476
5	13.45779166646818	37.51375504351596	33.22427747316873	15.80417581684713
6	33.637636836539286	34.30988652928231	17.55385333600099	14.498623298177419
7	28.616302167937768	31.10435731196677	21.560667482838948	18.718673037256515
8	28.992277160937256	31.665450346508333	20.481243580973576	18.861028911580828
9	27.85583285299848	14.520657574443963	18.649790637519473	38.973718935038086
10	16.03130671722489	27.384421907080213	31.76755286462179	24.81671851107311
11	36.42459377962396	21.712437417529284	21.49026071464299	20.37270808820377
12	24.134277806624333	24.475736341420426	29.388108973290205	22.001876878665033
13	30.723741562382394	19.84241841056006	25.496396297690104	23.937443729367438
14	24.46611376552355	19.24677143524054	26.01325247828966	30.273862320946254
15	25.729815217960873	25.816799493147485	25.391310147053918	23.06207514183772
16	25.2336332845853	25.636161830766518	25.492490103514147	23.637714781134033
17	23.186120625181612	25.202383731177626	27.47969493576216	24.131800707878604
18	24.8046188364305	23.752042415552367	28.986437884367128	22.456900863650006
19	24.24936762527212	26.617950391333967	26.83965072907685	22.293031254317057
20	24.57148573524578	23.113617850354654	29.149068944327205	23.165827470072358
21	25.73991415900116	24.75707759511821	25.404362551983343	24.098645693897286
22	24.104457348646886	24.965534981874306	26.343182976615235	24.586824692863573
23	23.153346703315027	24.550620941964436	26.611852917498318	25.68417943722222
24	23.159063085035942	26.627191875116115	26.46532299938549	23.748422040462454
25	25.032511921037713	23.95525978573096	26.003439356335416	25.00878893689591
26	22.87343454504747	24.852826988943566	27.561248648313907	24.71248981769506
27	24.9349523396674	25.33643288253312	26.903293112236394	22.825321665563088
28	23.746897672003545	23.937729548453483	27.513707407001615	24.801665372541358
29	22.84456681735684	25.413222943650766	28.308093920151673	23.43411631884072
30	24.559290787574493	24.807667573348326	27.637562344288142	22.995479294789043
31	22.908113927487697	25.18895023413347	26.826026685975336	25.0769091524035
32	22.921737970589216	25.778309189559984	26.87937958203722	24.42057325781358
33	22.851331202393258	25.02803408868966	27.14957389137922	24.97106081753786
34	23.000909857423913	25.798316525583193	27.937958203722317	23.26281541327058
35	24.71734874215784	25.382640301443864	26.940259047364982	22.95975190903331
36	23.586267345645783	26.256960885658074	26.565073860415485	23.591697908280658
37	24.684670093319934	25.86643674109078	26.740280960161584	22.708612205427706
38	23.764237363223657	25.8362351909986	25.889206994945766	24.510320450831973
39	23.468509882194898	25.4136040357655	26.989515203193555	24.128370878846052
40	24.582918498687615	25.799269255870012	25.876916774245796	23.740895471196584
41	22.439846991515935	25.925887110988317	27.54047912806124	24.09378676943451
42	25.35558276129819	26.03649909728805	26.23581027329068	22.372107868123074
43	24.0357654949672	24.524516132105582	26.039643107234557	25.400075265692657
44	23.464032049846846	25.39359669974229	27.41252745054139	23.729843799869478
45	24.026524011185053	25.53078986104429	26.322032364247843	24.120653763522814
46	23.034731782606002	25.333669964701343	27.198353682064376	24.43324457062828
47	24.065490679915968	25.09882194900035	27.308584576249384	23.527102794834295
48	23.685827660618415	27.734073922342954	24.95438803751852	23.62571037952011
49	22.80350414199492	25.80374708821806	27.646994374127654	23.74575439565936
50	22.678315382306845	27.93424255560372	24.547286385960568	24.840155676128866
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	55.5
2	64.0
3	310.0
4	556.0
5	608.0
6	660.0
7	631.0
8	602.0
9	766.0
10	930.0
11	1383.0
12	1836.0
13	3094.0
14	4352.0
15	5834.0
16	7316.0
17	8348.0
18	9380.0
19	9669.0
20	9958.0
21	10519.5
22	11081.0
23	10983.5
24	10886.0
25	10037.0
26	9188.0
27	10597.5
28	12007.0
29	13442.5
30	14878.0
31	16373.5
32	17869.0
33	20264.5
34	22660.0
35	25191.0
36	27722.0
37	31369.0
38	35016.0
39	37378.0
40	39740.0
41	43848.0
42	47956.0
43	51498.0
44	55040.0
45	66684.5
46	78329.0
47	87273.0
48	96217.0
49	92639.5
50	89062.0
51	80037.0
52	71012.0
53	67343.5
54	63675.0
55	64144.0
56	64613.0
57	62738.0
58	60863.0
59	56036.0
60	51209.0
61	45490.0
62	39771.0
63	34479.0
64	29187.0
65	24998.5
66	20810.0
67	17701.0
68	14592.0
69	13004.0
70	11416.0
71	9010.5
72	6605.0
73	5792.0
74	4979.0
75	3719.0
76	2459.0
77	2185.5
78	1912.0
79	1615.5
80	1319.0
81	1037.5
82	756.0
83	642.5
84	529.0
85	429.5
86	330.0
87	241.0
88	152.0
89	101.5
90	51.0
91	28.5
92	6.0
93	6.0
94	6.0
95	4.0
96	2.0
97	5.5
98	9.0
99	4.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016291687904612644
2	0.004573105376733374
3	2.8581908604583586E-4
4	2.8581908604583586E-4
5	0.0
6	4.7636514340972645E-4
7	0.0
8	9.52730286819453E-5
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1049615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.83721122336098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.0246559376912	39.40906148025443
2	13.947467150483387	14.45995601420992
3	4.529588183245717	7.044036582292446
4	1.8820717618224045	3.902454058204445
5	0.9882070372492107	2.5612948461149543
6	0.5759162340406723	1.791233488255736
7	0.3870765434990621	1.4045477981478551
8	0.25968255480602237	1.0768975555601437
9	0.1936274612605529	0.9033396847205786
>10	1.0200792270566452	9.83602319221056
>50	0.09434135209645894	3.4607597348952366
>100	0.08785383019795201	8.75728970460851
>500	0.0066583952122631854	2.3476131997799534
>1k	0.0024044204933172616	1.9425382275888932
>5k	3.6991084512573257E-4	1.1029544331564398
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	6368	0.6066986466466276	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	5136	0.48932227531047096	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2380	0.22674980826302976	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	2178	0.20750465646927682	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2076	0.1977868075437184	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1682	0.16024923424303197	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	1612	0.1535801222352958	No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA	1513	0.14414809239578322	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	1470	0.14005135216245956	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	1432	0.13643097707254564	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	1391	0.1325247828965859	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1343	0.12795167751985254	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1089	0.10375232823463841	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1053	0.10032249920208838	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	9.527302868194528E-5
11	0.0	0.0	0.0	0.0	9.527302868194528E-5
12	0.0	0.0	0.0	0.0	2.8581908604583586E-4
13	0.0	0.0	0.0	0.0	3.810921147277811E-4
14	0.0	0.0	0.0	9.527302868194528E-5	3.810921147277811E-4
15	0.0	0.0	0.0	9.527302868194528E-5	4.7636514340972645E-4
16	0.0	0.0	0.0	1.9054605736389056E-4	4.7636514340972645E-4
17	0.0	0.0	0.0	2.8581908604583586E-4	4.7636514340972645E-4
18	0.0	0.0	0.0	3.810921147277811E-4	4.7636514340972645E-4
19	0.0	0.0	0.0	5.716381720916717E-4	4.7636514340972645E-4
20	0.0	0.0	0.0	9.527302868194529E-4	4.7636514340972645E-4
21	0.0	0.0	0.0	0.002000733602320851	4.7636514340972645E-4
22	0.0	0.0	0.0	0.0040967402333236475	4.7636514340972645E-4
23	0.0	0.0	0.0	0.00809820743796535	4.7636514340972645E-4
24	0.0	0.0	0.0	0.015053138531747355	4.7636514340972645E-4
25	0.0	0.0	0.0	0.0201978820805724	4.7636514340972645E-4
26	0.0	0.0	0.0	0.028200816489855805	4.7636514340972645E-4
27	0.0	0.0	0.0	0.03610847787045726	5.716381720916717E-4
28	0.0	0.0	0.0	0.050971070344840726	5.716381720916717E-4
29	0.0	0.0	0.0	0.07402714328587148	5.716381720916717E-4
30	0.0	0.0	0.0	0.11918655888111356	5.716381720916717E-4
31	0.0	0.0	0.0	0.19731044240030868	5.716381720916717E-4
32	0.0	0.0	0.0	0.2913449217093887	5.716381720916717E-4
33	0.0	0.0	0.0	0.37061208157276715	5.716381720916717E-4
34	0.0	0.0	0.0	0.47474550192213333	6.66911200773617E-4
35	0.0	0.0	0.0	0.5876440409102385	6.66911200773617E-4
36	0.0	0.0	0.0	0.7497987357269094	6.66911200773617E-4
37	0.0	0.0	0.0	0.9826460178255837	6.66911200773617E-4
38	0.0	0.0	0.0	1.2534119653396723	6.66911200773617E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGT	30	0.005744886	29.33311	4
AACGCGC	30	0.005744886	29.33311	42
TATGCGT	70	3.215021E-5	21.999834	39
GTGTATA	445	0.0	21.758865	1
TGGTATA	310	0.0	21.290161	44
GATCGAA	65	4.93182E-4	20.307537	11
AATCGAG	110	1.2653436E-7	19.999847	22
TGATCGA	80	8.987655E-5	19.249855	10
GTATATC	460	0.0	19.13029	3
CGCAATA	255	0.0	18.980247	36
TGTATAT	495	0.0	18.667414	2
ACGACGG	240	0.0	18.333195	6
ACGGTAT	240	0.0	18.333195	9
TATATCA	485	0.0	18.144192	4
CGCTAGA	255	0.0	18.11751	37
ATAAGGT	85	1.430688E-4	18.11751	4
CGACGGT	245	0.0	17.95905	7
TACGACG	245	0.0	17.95905	5
GTCGTAT	135	5.523907E-8	17.925789	43
CGGTGAT	135	5.523907E-8	17.925789	43
>>END_MODULE