FastQCFastQC Report
Thu 2 Feb 2017
SRR4062643_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062643_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693786
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG28910.41669909741620614No Hit
TCCATGTACTCTGCGTTGATACCAC24830.35789133825127634No Hit
GTATCAACGCAGAGTACTTTTTTTT19730.28438163929511406No Hit
TATCAACGCAGAGTACTTTTTTTTT13200.19026039729830238No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12700.18305356406730605No Hit
GAGTACATGGAAGCAGTGGTATCAA12180.17555845750706991No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11530.16618957430677472No Hit
CATGTACTCTGCGTTGATACCACTG10460.15076695119244263No Hit
GCGCAAGACGGACCAGAGCGAAAGC9210.13274986811495187No Hit
GATTAAGAGGGACGGCCGGGGGCAT9110.13130850146875261No Hit
GTCTTGCGCCGGTCCAAGAATTTCA8600.1239575315731364No Hit
GAATAGGACCGCGGTTCTATTTTGT8580.12366925824389653No Hit
ACGCAGAGTACTTTTTTTTTTTTTT8390.12093066161611794No Hit
GAATAACGCCGCCGCATCGCCAGTC8010.11545346836056075No Hit
GTATCTGATCGTCTTCGAACCTCCG7820.11271487173278216No Hit
GAACTACGACGGTATCTGATCGTCT7800.1124265984035423No Hit
GTCCTATTCCATTATTCCTAGCTGC7760.11185005174506259No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7570.109111455117284No Hit
GGGTAGGCACACGCTGAGCCAGTCA7500.10810249846494452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGGA456.7815697E-414.7703122
TAGTACG653.383304E-614.6080022
CTGTACT400.005285311514.2458864
ACTCTAA954.8621587E-914.00404510
GCGTTAT954.9330993E-913.9898991
CTAACAG551.9636881E-413.8131943
GTCCGTA551.9697081E-413.8082121
TTGCATC500.001494826613.30384212
CGTCTTA500.001497269413.30096115
CCCAATT500.001498084313.29999913
TAAGGCT500.001501348113.296164
TGTAAGG500.001503799913.29328252
GATATAC1301.4551915E-1113.1443561
GTATCCT655.480977E-513.1443561
CGTTATT957.412382E-812.9934322
ACTGTTC1403.6379788E-1212.8965828
GTCCTAC2750.012.7725961
TATACAC1207.403287E-1012.6620953
GTTTTCG1600.012.4696515
GAACAGT1155.2787072E-912.3948836
Produced by FastQC (version 0.11.5)