##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062643_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	693786
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.092792301949014	32.0	32.0	32.0	32.0	32.0
2	30.785923901606548	32.0	32.0	32.0	32.0	32.0
3	30.815850997281583	32.0	32.0	32.0	32.0	32.0
4	30.712174935787115	32.0	32.0	32.0	32.0	32.0
5	30.721994966747673	32.0	32.0	32.0	32.0	32.0
6	34.19066830405918	36.0	36.0	36.0	32.0	36.0
7	34.05878325593194	36.0	36.0	36.0	32.0	36.0
8	34.01512570158521	36.0	36.0	36.0	32.0	36.0
9	34.19760560172733	36.0	36.0	36.0	32.0	36.0
10	33.87244193454466	36.0	36.0	36.0	32.0	36.0
11	34.18086412813173	36.0	36.0	36.0	32.0	36.0
12	33.99293442070033	36.0	36.0	36.0	32.0	36.0
13	34.10096773356626	36.0	36.0	36.0	32.0	36.0
14	33.987370745446	36.0	36.0	36.0	32.0	36.0
15	33.99491485847221	36.0	36.0	36.0	32.0	36.0
16	34.0105450383836	36.0	36.0	36.0	32.0	36.0
17	33.890353797856974	36.0	36.0	36.0	32.0	36.0
18	33.95470937724313	36.0	36.0	36.0	32.0	36.0
19	33.92141813181586	36.0	36.0	36.0	32.0	36.0
20	33.83850495685989	36.0	36.0	36.0	32.0	36.0
21	33.774919067262815	36.0	36.0	36.0	32.0	36.0
22	33.79097877443477	36.0	36.0	36.0	32.0	36.0
23	33.70386689843842	36.0	36.0	36.0	32.0	36.0
24	33.67184837975975	36.0	36.0	36.0	27.0	36.0
25	33.07477666023817	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	19.0
5	65.0
6	114.0
7	29.0
8	110.0
9	126.0
10	77.0
11	27.0
12	59.0
13	43.0
14	214.0
15	443.0
16	697.0
17	916.0
18	1097.0
19	1345.0
20	1586.0
21	1847.0
22	2281.0
23	2907.0
24	3947.0
25	5196.0
26	6983.0
27	9436.0
28	13064.0
29	18045.0
30	25295.0
31	36721.0
32	56620.0
33	86160.0
34	186381.0
35	231931.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.78285117921027	17.509481395419236	10.949408052447145	25.758259372923355
2	17.06637146456566	20.458889359751613	36.30236305822458	26.172376117458146
3	19.519461949596693	24.409741641249763	27.980264182056146	28.0905322270974
4	12.795396194537306	15.127860259742507	35.659022008485096	36.417721537235096
5	14.829980621943342	36.43882070683769	32.97830926455661	15.75288940666236
6	33.8031398301602	35.28177574742926	16.836030610964748	14.079053811445801
7	29.792653447410476	30.52967409552711	21.03032890447866	18.647343552583756
8	28.58195971620729	31.89508671604283	19.90270953371553	19.62024403403435
9	27.730143134768287	13.868822705349556	18.581117637129342	39.81991652275282
10	16.47098720002309	25.91324299753236	30.86237499458851	26.75339480785604
11	38.10296210138047	21.18380152217896	21.510951664901278	19.202284711539296
12	24.48594030311734	23.76805360913422	28.59091283031231	23.155093257436132
13	29.860593990821716	19.346119433139954	25.368285854475136	25.425000721563194
14	23.832353650035863	19.530791111701216	24.50891961593913	32.12793562232379
15	25.79570362610263	26.60695552544506	22.54752143930113	25.049819409151187
16	26.325814420612748	25.49537667674832	23.637822362801007	24.540986539837924
17	23.985539664901218	25.208895559436016	25.662332414529605	25.143232361133162
18	25.319709335469298	23.848057786533314	27.02242042445103	23.809812453546353
19	25.85837622207661	24.93815650085585	25.06516298684617	24.138304290221367
20	25.623982637004882	23.757806998302954	25.66626453169555	24.951945832996618
21	27.031411232420027	23.983085704389488	23.7364429467243	25.249060116466183
22	25.739691521533587	24.578819351718863	24.783076267944658	24.898412858802896
23	24.278512035263603	24.131291685369906	25.300683131290242	26.28951314807625
24	24.539060932716968	25.27656344036982	24.97780997836563	25.20656564854758
25	25.022189573281878	24.257280705678944	24.990294316727113	25.73023540431206
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	90.0
1	90.0
2	251.5
3	413.0
4	413.0
5	413.0
6	1183.0
7	1953.0
8	1953.0
9	1953.0
10	2054.5
11	2156.0
12	2156.0
13	2156.0
14	2026.5
15	1897.0
16	1897.0
17	1897.0
18	3223.5
19	4550.0
20	4550.0
21	4550.0
22	7479.0
23	10408.0
24	10408.0
25	10408.0
26	15829.0
27	21250.0
28	21250.0
29	21250.0
30	26182.5
31	31115.0
32	31115.0
33	31115.0
34	37557.5
35	44000.0
36	44000.0
37	44000.0
38	48365.5
39	52731.0
40	52731.0
41	52731.0
42	60530.0
43	68329.0
44	68329.0
45	68329.0
46	81184.0
47	94039.0
48	94039.0
49	94039.0
50	90738.5
51	87438.0
52	87438.0
53	87438.0
54	79781.0
55	72124.0
56	72124.0
57	72124.0
58	67512.0
59	62900.0
60	62900.0
61	62900.0
62	57799.0
63	52698.0
64	52698.0
65	52698.0
66	45662.5
67	38627.0
68	38627.0
69	38627.0
70	29803.0
71	20979.0
72	20979.0
73	20979.0
74	16876.5
75	12774.0
76	12774.0
77	12774.0
78	10263.5
79	7753.0
80	7753.0
81	7753.0
82	5543.0
83	3333.0
84	3333.0
85	3333.0
86	2386.5
87	1440.0
88	1440.0
89	1440.0
90	943.5
91	447.0
92	447.0
93	447.0
94	252.5
95	58.0
96	58.0
97	58.0
98	171.0
99	284.0
100	284.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008504063212575637
2	0.0034592799508782247
3	0.003171006621638373
4	0.014269529797372678
5	0.031421792887143876
6	0.04453822936755714
7	0.06543804573744641
8	0.08705854543043533
9	0.09556260864301096
10	0.11761551832985964
11	0.11992170496377846
12	0.132461594785712
13	0.12193961826845742
14	0.12611958154243527
15	0.11285900839740207
16	0.11213832507430245
17	0.12338098491465668
18	0.1284257681763541
19	0.13087609147489282
20	0.11790379165909949
21	0.12698440153015483
22	0.14860490122314374
23	0.13649742139506996
24	0.13073195481027292
25	0.12871404150559396
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	693786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.88638380182853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.20318553231802	37.74184558656674
2	13.543395935636166	13.241753033792683
3	4.1373749713400345	6.067839023430247
4	1.6618093796563669	3.2495940455743897
5	0.8392212948891777	2.051324715830993
6	0.4804521789041484	1.409254176979979
7	0.323984191661519	1.1086890877503133
8	0.23435666810563807	0.9165480018823974
9	0.1677922815230091	0.7382482086166455
>10	1.1204698160813507	10.677199372990083
>50	0.1402795383856034	4.780465649930292
>100	0.13613622703314882	13.018309935167233
>500	0.00917439790875393	3.0668933886670207
>1k	0.002367586557097789	1.932035772820992
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	2891	0.41669909741620614	No Hit
TCCATGTACTCTGCGTTGATACCAC	2483	0.35789133825127634	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1973	0.28438163929511406	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1320	0.19026039729830238	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1270	0.18305356406730605	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1218	0.17555845750706991	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1153	0.16618957430677472	No Hit
CATGTACTCTGCGTTGATACCACTG	1046	0.15076695119244263	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	921	0.13274986811495187	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	911	0.13130850146875261	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	860	0.1239575315731364	No Hit
GAATAGGACCGCGGTTCTATTTTGT	858	0.12366925824389653	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	839	0.12093066161611794	No Hit
GAATAACGCCGCCGCATCGCCAGTC	801	0.11545346836056075	No Hit
GTATCTGATCGTCTTCGAACCTCCG	782	0.11271487173278216	No Hit
GAACTACGACGGTATCTGATCGTCT	780	0.1124265984035423	No Hit
GTCCTATTCCATTATTCCTAGCTGC	776	0.11185005174506259	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	757	0.109111455117284	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	750	0.10810249846494452	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.4413666461992602E-4	0.0
8	0.0	0.0	0.0	1.4413666461992602E-4	0.0
9	0.0	0.0	0.0	1.4413666461992602E-4	0.0
10	0.0	0.0	0.0	1.4413666461992602E-4	0.0
11	0.0	0.0	0.0	1.4413666461992602E-4	0.0
12	0.0	0.0	0.0	1.4413666461992602E-4	0.0
13	0.0	0.0	0.0	1.4413666461992602E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGGA	45	6.7815697E-4	14.770312	2
TAGTACG	65	3.383304E-6	14.608002	2
CTGTACT	40	0.0052853115	14.245886	4
ACTCTAA	95	4.8621587E-9	14.004045	10
GCGTTAT	95	4.9330993E-9	13.989899	1
CTAACAG	55	1.9636881E-4	13.813194	3
GTCCGTA	55	1.9697081E-4	13.808212	1
TTGCATC	50	0.0014948266	13.303842	12
CGTCTTA	50	0.0014972694	13.300961	15
CCCAATT	50	0.0014980843	13.299999	13
TAAGGCT	50	0.0015013481	13.29616	4
TGTAAGG	50	0.0015037999	13.2932825	2
GATATAC	130	1.4551915E-11	13.144356	1
GTATCCT	65	5.480977E-5	13.144356	1
CGTTATT	95	7.412382E-8	12.993432	2
ACTGTTC	140	3.6379788E-12	12.896582	8
GTCCTAC	275	0.0	12.772596	1
TATACAC	120	7.403287E-10	12.662095	3
GTTTTCG	160	0.0	12.46965	15
GAACAGT	115	5.2787072E-9	12.394883	6
>>END_MODULE