FastQCFastQC Report
Thu 2 Feb 2017
SRR4062643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences693786
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA14010.20193546713251637No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC11350.16359511434361604No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9320.13433537142577107No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC9110.13130850146875261No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG8560.12338098491465668No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8350.12035411495763823No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8220.1184803383175792No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7740.11156177841582275No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC7140.10291357853862719No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA7030.101328075227808No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT654.929577E-420.3074936
TACACTA654.929577E-420.3074935
GTTTAGA1201.4253601E-820.170831
TCTACAC1201.4277248E-820.1679233
TTAATAC550.004480106320.0012443
TAACTGG550.004481975519.9998026
CGCGGGA2050.019.31688344
GTATGGC708.115162E-418.8583163
ATTGTCG708.119096E-418.85695843
TTAGGAC2350.018.724573
CATACGC951.595647E-518.52613438
GCTTCGG2300.018.17373533
ACCGCCT851.4298275E-418.1174688
CGTCGTA2100.017.8093510
GTAAACG2100.017.8093527
TTTAGAT1254.8419497E-717.6010952
TAAACGC2300.017.21722428
GCACACG3450.017.2172227
GTTCTAC2950.017.1560841
CTAAGGT1053.7685957E-516.7629474
Produced by FastQC (version 0.11.5)