FastQCFastQC Report
Thu 2 Feb 2017
SRR4062642_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062642_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113974
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31910.28645192796241203No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21320.1913868725840998No Hit
TATCAACGCAGAGTACTTTTTTTTT21090.18932219243896176No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15850.14228339261059952No Hit
GTACATGGAAGCAGTGGTATCAACG15590.13994940635957392No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13210.11858445529249337No Hit
TCCATGTACTCTGCGTTGATACCAC12270.11014619730801616No Hit
GCGCAAGACGGACCAGAGCGAAAGC11800.10592706831577757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTTA759.645246E-713.93593716
CGACCAT2150.013.25650410
ATCGCTC655.4402288E-513.15630316
GACCATA2250.013.0895711
CCGTTAA801.991746E-613.06611617
CAAGACG2050.012.5094734
CGCCAGT1700.012.29752118
CCGACCA2400.012.270379
AATCGCT701.0884628E-412.21656915
AAATCGC701.0889196E-412.21601914
CAAATCG701.0893766E-412.2154713
TTAATCC701.09258035E-412.2116294
AAGACGG1950.012.1779335
TAGAACA1251.4133548E-912.1573544
GTAAACG1103.794412E-812.09425617
AATCGTA550.003065277112.09208213
GCACCGT951.0393542E-611.9995476
AGATTAT951.0404838E-611.9984685
ATCGCCA1750.011.94508816
CTACATT802.8710316E-511.87241654
Produced by FastQC (version 0.11.5)