##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062642_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113974
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.060559761717958	32.0	32.0	32.0	32.0	32.0
2	30.67651848247805	32.0	32.0	32.0	32.0	32.0
3	30.66662148308668	32.0	32.0	32.0	32.0	32.0
4	30.7428871769	32.0	32.0	32.0	32.0	32.0
5	30.53401425886062	32.0	32.0	32.0	32.0	32.0
6	34.16405230283651	36.0	36.0	36.0	32.0	36.0
7	34.03258693649941	36.0	36.0	36.0	32.0	36.0
8	34.01643575164232	36.0	36.0	36.0	32.0	36.0
9	34.201650128279475	36.0	36.0	36.0	32.0	36.0
10	33.828725805090606	36.0	36.0	36.0	32.0	36.0
11	34.20410979071325	36.0	36.0	36.0	32.0	36.0
12	33.93971133976197	36.0	36.0	36.0	32.0	36.0
13	34.08759899243609	36.0	36.0	36.0	32.0	36.0
14	33.982954718871355	36.0	36.0	36.0	32.0	36.0
15	33.917398431202166	36.0	36.0	36.0	32.0	36.0
16	33.91439656580854	36.0	36.0	36.0	32.0	36.0
17	33.84902609935241	36.0	36.0	36.0	32.0	36.0
18	33.85628659196714	36.0	36.0	36.0	32.0	36.0
19	33.846049369195335	36.0	36.0	36.0	32.0	36.0
20	33.82829132457311	36.0	36.0	36.0	32.0	36.0
21	33.81022716867719	36.0	36.0	36.0	32.0	36.0
22	33.76175206961742	36.0	36.0	36.0	32.0	36.0
23	33.735084481325416	36.0	36.0	36.0	27.0	36.0
24	33.711858625066654	36.0	36.0	36.0	27.0	36.0
25	33.25891537863541	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	41.0
5	113.0
6	172.0
7	52.0
8	144.0
9	170.0
10	144.0
11	49.0
12	79.0
13	56.0
14	238.0
15	345.0
16	494.0
17	641.0
18	856.0
19	1213.0
20	1904.0
21	2760.0
22	4145.0
23	5862.0
24	8374.0
25	11192.0
26	15286.0
27	19523.0
28	25875.0
29	33702.0
30	42851.0
31	57574.0
32	81613.0
33	115372.0
34	250270.0
35	432859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.198011979832515	17.785795854460837	11.123540227243346	25.892651938463306
2	16.877359862542214	20.382659061243764	36.75720998041191	25.982771095802114
3	19.150785108126865	24.12796934797109	28.442899554098446	28.278345989803604
4	12.849712741974148	15.393331160605022	35.6464809432873	36.110475154133525
5	14.709511263409143	36.3989597757196	33.35344816748473	15.53808079338653
6	33.79317715227923	35.34576746709008	16.9647972957196	13.8962580849111
7	29.879690792582043	30.875920014227997	20.82371476460031	18.42067442858965
8	27.963946933854196	32.41451682170947	19.944547566723507	19.676988677712828
9	27.387399043468026	14.573859884148757	19.09128563971108	38.947455432672136
10	16.106442828769403	26.462240835437807	31.613268506618976	25.818047829173818
11	36.774874229153674	21.623688595158967	22.45856616741767	19.14287100826969
12	24.629223339627913	24.316621382225552	28.487313915101335	22.5668413630452
13	29.096824055934	19.840034509409207	25.47064004169887	25.59250139295792
14	23.439617642512285	20.084013675139932	25.294335457941035	31.182033224406748
15	24.996742360723566	27.17940560889097	22.869526379688867	24.954325650696596
16	25.546204894793835	26.017891563287698	23.978558879957586	24.457344661960885
17	23.58705157137067	25.97486633755463	25.753331338170256	24.684750752904446
18	24.456354195970437	24.937018425761735	26.773213305087868	23.83341407317996
19	25.179649841225043	25.539623631897364	25.799739524582932	23.480987002294665
20	25.253679943130397	24.569231640276403	25.704646537631625	24.472441878961572
21	26.21974401216576	24.529695829075408	24.90987578091928	24.34068437783955
22	25.434619820587574	24.8267498734728	25.196757667368747	24.541872638570887
23	24.122148245824558	24.682768892520293	25.829358113179396	25.365724748475753
24	24.17922977431796	25.63125627481276	25.656872341209603	24.532641609659684
25	24.386199383974898	25.030266479839874	25.800163039033198	24.783371097152028
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	422.0
1	422.0
2	709.5
3	997.0
4	997.0
5	997.0
6	2397.5
7	3798.0
8	3798.0
9	3798.0
10	3862.5
11	3927.0
12	3927.0
13	3927.0
14	4166.0
15	4405.0
16	4405.0
17	4405.0
18	6871.5
19	9338.0
20	9338.0
21	9338.0
22	14266.0
23	19194.0
24	19194.0
25	19194.0
26	27890.0
27	36586.0
28	36586.0
29	36586.0
30	43858.5
31	51131.0
32	51131.0
33	51131.0
34	61602.5
35	72074.0
36	72074.0
37	72074.0
38	80535.0
39	88996.0
40	88996.0
41	88996.0
42	100374.0
43	111752.0
44	111752.0
45	111752.0
46	128739.5
47	145727.0
48	145727.0
49	145727.0
50	146151.5
51	146576.0
52	146576.0
53	146576.0
54	134658.0
55	122740.0
56	122740.0
57	122740.0
58	112281.0
59	101822.0
60	101822.0
61	101822.0
62	90632.0
63	79442.0
64	79442.0
65	79442.0
66	66195.5
67	52949.0
68	52949.0
69	52949.0
70	40584.5
71	28220.0
72	28220.0
73	28220.0
74	22223.5
75	16227.0
76	16227.0
77	16227.0
78	13158.0
79	10089.0
80	10089.0
81	10089.0
82	7126.0
83	4163.0
84	4163.0
85	4163.0
86	3134.5
87	2106.0
88	2106.0
89	2106.0
90	1377.0
91	648.0
92	648.0
93	648.0
94	404.0
95	160.0
96	160.0
97	160.0
98	322.5
99	485.0
100	485.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009156407600177382
2	0.0028725984628007475
3	0.004578203800088691
4	0.014991373227741403
5	0.0330348823222086
6	0.04308897694201121
7	0.0606836425266658
8	0.08510072946047215
9	0.08931985845271075
10	0.11346763928062953
11	0.11095411562567886
12	0.12361150260239467
13	0.11077457822175384
14	0.11651977514735533
15	0.10817128586484066
16	0.10404192557456457
17	0.11517324461791748
18	0.12091844154351895
19	0.12504780183379505
20	0.11248018355904177
21	0.12055936673566889
22	0.14048801857134907
23	0.13079299875939654
24	0.12486826442987
25	0.12181612856314421
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1113974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.41237155722111
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.99197922155024	45.43370017336494
2	11.141920606824883	12.348004890528777
3	2.9651831128569057	4.92923485154473
4	1.1695133931923551	2.592220427388848
5	0.6306811089227826	1.7473767970874732
6	0.40933309935718093	1.3609270675349405
7	0.26736951447635	1.037090521546612
8	0.20537262533482917	0.9104147378188413
9	0.13848661821164945	0.690648474964225
>10	0.8614949433644794	9.067633923641216
>50	0.10455917374220694	4.050904112707281
>100	0.10534163935125827	11.666094913383725
>500	0.0066548639891102004	2.4068452833394525
>1k	0.0021100788258154295	1.758903825148966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3191	0.28645192796241203	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2132	0.1913868725840998	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2109	0.18932219243896176	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1585	0.14228339261059952	No Hit
GTACATGGAAGCAGTGGTATCAACG	1559	0.13994940635957392	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1321	0.11858445529249337	No Hit
TCCATGTACTCTGCGTTGATACCAC	1227	0.11014619730801616	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1180	0.10592706831577757	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	8.976870196252336E-5	0.0
6	0.0	0.0	0.0	8.976870196252336E-5	0.0
7	0.0	0.0	0.0	8.976870196252336E-5	0.0
8	0.0	0.0	0.0	8.976870196252336E-5	0.0
9	0.0	0.0	0.0	8.976870196252336E-5	0.0
10	0.0	0.0	0.0	8.976870196252336E-5	0.0
11	0.0	0.0	0.0	8.976870196252336E-5	0.0
12	0.0	0.0	0.0	8.976870196252336E-5	0.0
13	0.0	0.0	0.0	1.7953740392504672E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTTA	75	9.645246E-7	13.935937	16
CGACCAT	215	0.0	13.256504	10
ATCGCTC	65	5.4402288E-5	13.156303	16
GACCATA	225	0.0	13.08957	11
CCGTTAA	80	1.991746E-6	13.066116	17
CAAGACG	205	0.0	12.509473	4
CGCCAGT	170	0.0	12.297521	18
CCGACCA	240	0.0	12.27037	9
AATCGCT	70	1.0884628E-4	12.216569	15
AAATCGC	70	1.0889196E-4	12.216019	14
CAAATCG	70	1.0893766E-4	12.21547	13
TTAATCC	70	1.09258035E-4	12.211629	4
AAGACGG	195	0.0	12.177933	5
TAGAACA	125	1.4133548E-9	12.157354	4
GTAAACG	110	3.794412E-8	12.094256	17
AATCGTA	55	0.0030652771	12.092082	13
GCACCGT	95	1.0393542E-6	11.999547	6
AGATTAT	95	1.0404838E-6	11.998468	5
ATCGCCA	175	0.0	11.945088	16
CTACATT	80	2.8710316E-5	11.8724165	4
>>END_MODULE