FastQCFastQC Report
Thu 2 Feb 2017
SRR4062642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113974
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAT450.001398161224.44439535
TGCGTAG654.931898E-420.3076513
CTAGCGG3750.019.94662729
TAGCGGC3900.019.1794530
CGAATGC3800.019.10522343
TCTAGCG3950.018.9366728
CCCCTAT1051.7916955E-618.857951
TTAACGG1404.0799932E-918.85710535
ATTTCGT1404.0799932E-918.85710542
CGCAATA3800.018.52627836
ATCGTTT3000.018.33329429
ATAACGC3150.018.1586933
ATACGAA4000.018.14996340
GGCGAAT851.4307276E-418.11760937
AATAACG3050.018.0335622
TAACGCC3050.018.0327514
TCTACAC2850.017.7543493
TAGTACG750.001290413717.6007542
CATCGTT3250.017.59996428
TAACGGC1509.59335E-917.59996236