##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062642_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113974
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277273975873765	32.0	32.0	32.0	32.0	32.0
2	31.42802345476645	32.0	32.0	32.0	32.0	32.0
3	31.50792657638329	32.0	32.0	32.0	32.0	32.0
4	31.60388483034613	32.0	32.0	32.0	32.0	32.0
5	31.550598128861175	32.0	32.0	32.0	32.0	32.0
6	35.1257156809764	36.0	36.0	36.0	36.0	36.0
7	35.138726756638846	36.0	36.0	36.0	36.0	36.0
8	35.0810952499789	36.0	36.0	36.0	36.0	36.0
9	35.20511071174013	36.0	36.0	36.0	36.0	36.0
10	35.08469677030164	36.0	36.0	36.0	36.0	36.0
11	35.210272412102974	36.0	36.0	36.0	36.0	36.0
12	35.12487903667411	36.0	36.0	36.0	36.0	36.0
13	35.16402716759996	36.0	36.0	36.0	36.0	36.0
14	35.12072454114728	36.0	36.0	36.0	36.0	36.0
15	35.08857747128748	36.0	36.0	36.0	36.0	36.0
16	35.104577844725284	36.0	36.0	36.0	36.0	36.0
17	35.07815352961559	36.0	36.0	36.0	36.0	36.0
18	35.08802539377041	36.0	36.0	36.0	36.0	36.0
19	35.0598802126441	36.0	36.0	36.0	36.0	36.0
20	35.05287556082997	36.0	36.0	36.0	36.0	36.0
21	35.05257932411349	36.0	36.0	36.0	36.0	36.0
22	35.02835254682784	36.0	36.0	36.0	36.0	36.0
23	35.002113155244196	36.0	36.0	36.0	36.0	36.0
24	34.97711975324379	36.0	36.0	36.0	36.0	36.0
25	34.95399443793123	36.0	36.0	36.0	32.0	36.0
26	34.91310838493538	36.0	36.0	36.0	32.0	36.0
27	34.896151077134654	36.0	36.0	36.0	32.0	36.0
28	34.886761270909375	36.0	36.0	36.0	32.0	36.0
29	34.85435297412686	36.0	36.0	36.0	32.0	36.0
30	34.83577175050764	36.0	36.0	36.0	32.0	36.0
31	34.833690014309134	36.0	36.0	36.0	32.0	36.0
32	34.7942869402697	36.0	36.0	36.0	32.0	36.0
33	34.763230560138744	36.0	36.0	36.0	32.0	36.0
34	34.753296755579576	36.0	36.0	36.0	32.0	36.0
35	34.72031483679152	36.0	36.0	36.0	32.0	36.0
36	34.69075400323526	36.0	36.0	36.0	32.0	36.0
37	34.6756701682445	36.0	36.0	36.0	32.0	36.0
38	34.63759836405517	36.0	36.0	36.0	32.0	36.0
39	34.62584943634232	36.0	36.0	36.0	32.0	36.0
40	34.61138680076914	36.0	36.0	36.0	32.0	36.0
41	34.571925377073434	36.0	36.0	36.0	32.0	36.0
42	34.54589963500046	36.0	36.0	36.0	32.0	36.0
43	34.52733008131249	36.0	36.0	36.0	32.0	36.0
44	34.49084808083492	36.0	36.0	36.0	32.0	36.0
45	34.4434591830689	36.0	36.0	36.0	32.0	36.0
46	34.43670139518517	36.0	36.0	36.0	32.0	36.0
47	34.39386107754759	36.0	36.0	36.0	32.0	36.0
48	34.34346133751775	36.0	36.0	36.0	32.0	36.0
49	34.330780610678524	36.0	36.0	36.0	32.0	36.0
50	33.80818852145562	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	8.0
21	41.0
22	92.0
23	280.0
24	789.0
25	1803.0
26	3728.0
27	7060.0
28	11835.0
29	18121.0
30	25809.0
31	37034.0
32	53739.0
33	85711.0
34	183693.0
35	684229.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7835615134677	18.3309945206242	11.67431157653933	26.211132389368775
2	16.346253113988823	20.85508449850753	36.391049666719034	26.407612720784613
3	18.546061059259387	24.386988124423794	28.733526217563	28.333424598753826
4	12.563230886206398	15.873275205054341	35.9184648101884	35.645029098550864
5	14.36909658573719	36.67168174481631	33.26307436259734	15.696147306849173
6	33.904982993243074	35.59713062033145	16.701272656599958	13.796613729825516
7	29.91173941223045	30.79578158915738	20.77310601504164	18.51937298357053
8	28.026417133613528	32.52014858515549	19.820121475007497	19.633312806223486
9	27.453423508986745	14.263349054825337	19.05439444726717	39.22883298892075
10	16.00629817212969	26.582128487738494	31.60334083201224	25.808232508119577
11	37.035604062572375	21.47554610789839	22.446753694430928	19.042096135098305
12	24.6435733688578	24.16438803778185	28.562785127839607	22.62925346552074
13	29.302209925904915	19.62074518794873	25.573756658593467	25.50328822755289
14	23.471642964737057	19.94095014784905	25.33685705411437	31.25054983329952
15	25.105164034349098	27.074868892810784	22.819473344979325	25.000493727860796
16	25.59458299744877	26.00006822421349	24.052177160328696	24.353171618009036
17	23.73179266302445	25.797550032586038	25.71253907182753	24.758118232561984
18	24.661527109250304	24.647612960446114	26.93922838414541	23.75163154615817
19	25.29592252601946	25.177248302025003	26.052762452265494	23.474066719690047
20	25.378958575334792	24.28746092817247	25.930587248894497	24.40299324759824
21	26.395319818954484	24.279022670187995	25.04798137119897	24.277676139658556
22	25.392872724138982	24.734239757839948	25.402118900441124	24.470768617579942
23	24.086019960968567	24.41699716510439	26.124936488643364	25.37204638528368
24	24.422832130731955	25.276083642885737	25.797819338691923	24.503264887690378
25	24.601023004127565	24.520680015871108	25.963083519004932	24.915213460996398
26	23.946339860714883	25.22931414916327	26.49388585370933	24.33046013641252
27	25.01135574079826	25.0926861847763	25.52788485189062	24.368073222534818
28	24.227585203963468	24.769249551605334	26.35824534504396	24.644919899387236
29	24.142035630993185	25.244933903304744	26.129694229847377	24.483336235854697
30	24.208374701743487	25.162256928797262	26.417043844829415	24.21232452462984
31	24.48414415417236	24.90067093127847	25.5961988340841	25.018986080465073
32	24.18530414533912	25.110909231274697	25.569178454793377	25.134608168592802
33	23.99328889184128	24.689175869454765	26.106892979548896	25.21064225915506
34	24.517089267792606	24.89573365267053	26.470545991199074	24.11663108833779
35	25.25364146739511	24.762875973765993	25.916134487878534	24.067348070960364
36	24.156488392009148	25.13909660369093	25.890640176521174	24.813774827778744
37	25.095020171027333	24.97661525313876	25.52267826717679	24.405686308657117
38	24.189074430821545	24.844745029955817	26.1008784765174	24.865302062705233
39	24.512151989184666	24.770326776028885	25.638749198814338	25.078772035972115
40	24.82580383384172	24.88083204814475	26.23158170657484	24.06178241143869
41	23.824344194747813	25.05857407803055	26.63563063410816	24.481451093113485
42	24.79932206676278	25.32617457858083	26.05518620721848	23.819317147437914
43	24.01348684978285	24.387912105668537	26.362195167930313	25.236405876618306
44	23.789603707088318	24.816916732347433	26.226105815755123	25.167373744809122
45	23.78870602006869	25.190803376021343	26.250343365285005	24.770147238624958
46	23.733340036069098	24.79476612090239	26.28115762231221	25.190736220716303
47	23.92317953560855	24.659372660403207	26.62225509751574	24.795192706472502
48	24.258914480948388	25.624386206500333	25.608945989762777	24.507753322788503
49	23.38600362306481	25.311003667949162	26.224759285225684	25.078233423760338
50	23.180433295570634	25.784264264695583	25.844858138520287	25.190444301213493
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	126.0
1	105.5
2	85.0
3	250.0
4	415.0
5	464.0
6	513.0
7	421.5
8	330.0
9	446.5
10	563.0
11	836.0
12	1109.0
13	1842.0
14	2575.0
15	3354.5
16	4134.0
17	4520.0
18	4906.0
19	5114.0
20	5322.0
21	6027.0
22	6732.0
23	6923.5
24	7115.0
25	8300.5
26	9486.0
27	11437.0
28	13388.0
29	15173.0
30	16958.0
31	18135.5
32	19313.0
33	22476.0
34	25639.0
35	29086.5
36	32534.0
37	38376.0
38	44218.0
39	46884.0
40	49550.0
41	54275.5
42	59001.0
43	60140.5
44	61280.0
45	66829.0
46	72378.0
47	76582.0
48	80786.0
49	81388.5
50	81991.0
51	78613.5
52	75236.0
53	73825.0
54	72414.0
55	72863.5
56	73313.0
57	71626.5
58	69940.0
59	63905.0
60	57870.0
61	51178.5
62	44487.0
63	39239.0
64	33991.0
65	29105.5
66	24220.0
67	21664.5
68	19109.0
69	17404.0
70	15699.0
71	12439.5
72	9180.0
73	8156.5
74	7133.0
75	5370.5
76	3608.0
77	3182.5
78	2757.0
79	2327.5
80	1898.0
81	1495.5
82	1093.0
83	930.0
84	767.0
85	609.0
86	451.0
87	348.5
88	246.0
89	155.0
90	64.0
91	45.0
92	26.0
93	18.0
94	10.0
95	9.0
96	8.0
97	6.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014452761015966261
2	0.004398666396163644
3	6.283809137376635E-4
4	8.976870196252336E-5
5	0.0
6	4.488435098126168E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	8.976870196252336E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1113974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.19188068923001
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.52759626348896	48.849636315973825
2	11.103650630957791	13.144919267250943
3	2.783047398126728	4.942014286271679
4	1.106829530183985	2.620612859758678
5	0.5775615907666236	1.709347838566994
6	0.36914299341319906	1.3110160814027578
7	0.25437559939773774	1.0539879090861584
8	0.19542966742660461	0.9254279645961184
9	0.13426604606540157	0.7152713801386119
>10	0.7690412727357375	8.62424061544719
>50	0.08840318749996967	3.7355499227007707
>100	0.08425658714954756	9.738773218765093
>500	0.005789782046150048	2.2455496540557007
>1k	6.09450741700005E-4	0.38365268598549945
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	8.976870196252336E-5	0.0
9	0.0	0.0	0.0	8.976870196252336E-5	0.0
10	0.0	0.0	0.0	8.976870196252336E-5	0.0
11	0.0	0.0	0.0	8.976870196252336E-5	0.0
12	0.0	0.0	0.0	8.976870196252336E-5	8.976870196252336E-5
13	0.0	0.0	0.0	8.976870196252336E-5	8.976870196252336E-5
14	0.0	0.0	0.0	8.976870196252336E-5	8.976870196252336E-5
15	0.0	0.0	0.0	1.7953740392504672E-4	8.976870196252336E-5
16	0.0	0.0	0.0	1.7953740392504672E-4	8.976870196252336E-5
17	0.0	0.0	0.0	1.7953740392504672E-4	8.976870196252336E-5
18	0.0	0.0	0.0	2.6930610588757006E-4	8.976870196252336E-5
19	0.0	0.0	0.0	3.5907480785009343E-4	8.976870196252336E-5
20	0.0	0.0	0.0	3.5907480785009343E-4	8.976870196252336E-5
21	0.0	0.0	0.0	5.386122117751401E-4	8.976870196252336E-5
22	0.0	0.0	0.0	0.0016158366353254206	8.976870196252336E-5
23	0.0	0.0	0.0	0.002782829760838224	8.976870196252336E-5
24	0.0	0.0	0.0	0.005296353415788878	8.976870196252336E-5
25	0.0	0.0	0.0	0.006553115243264206	8.976870196252336E-5
26	0.0	0.0	0.0	0.009156407600177384	1.7953740392504672E-4
27	1.7953740392504672E-4	0.0	0.0	0.012747155678678318	1.7953740392504672E-4
28	1.7953740392504672E-4	0.0	0.0	0.02477616174165645	1.7953740392504672E-4
29	1.7953740392504672E-4	0.0	0.0	0.049193248675462806	1.7953740392504672E-4
30	1.7953740392504672E-4	0.0	0.0	0.09515482408027476	1.7953740392504672E-4
31	1.7953740392504672E-4	0.0	0.0	0.19282317181550018	1.7953740392504672E-4
32	1.7953740392504672E-4	0.0	0.0	0.30790664773145515	1.7953740392504672E-4
33	1.7953740392504672E-4	0.0	0.0	0.41868122595320895	1.7953740392504672E-4
34	1.7953740392504672E-4	0.0	0.0	0.5406768919202782	1.7953740392504672E-4
35	1.7953740392504672E-4	0.0	0.0	0.6824216723191026	1.7953740392504672E-4
36	1.7953740392504672E-4	0.0	0.0	0.8796435105307664	1.7953740392504672E-4
37	2.6930610588757006E-4	0.0	0.0	1.1611581598852396	1.7953740392504672E-4
38	2.6930610588757006E-4	0.0	0.0	1.525349783747197	1.7953740392504672E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGAAT	45	0.0013981612	24.444395	35
TGCGTAG	65	4.931898E-4	20.30765	13
CTAGCGG	375	0.0	19.946627	29
TAGCGGC	390	0.0	19.17945	30
CGAATGC	380	0.0	19.105223	43
TCTAGCG	395	0.0	18.93667	28
CCCCTAT	105	1.7916955E-6	18.85795	1
TTAACGG	140	4.0799932E-9	18.857105	35
ATTTCGT	140	4.0799932E-9	18.857105	42
CGCAATA	380	0.0	18.526278	36
ATCGTTT	300	0.0	18.333294	29
ATAACGC	315	0.0	18.158693	3
ATACGAA	400	0.0	18.149963	40
GGCGAAT	85	1.4307276E-4	18.117609	37
AATAACG	305	0.0	18.033562	2
TAACGCC	305	0.0	18.032751	4
TCTACAC	285	0.0	17.754349	3
TAGTACG	75	0.0012904137	17.600754	2
CATCGTT	325	0.0	17.599964	28
TAACGGC	150	9.59335E-9	17.599962	36
>>END_MODULE