FastQCFastQC Report
Thu 2 Feb 2017
SRR4062641_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062641_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9312
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT420.4510309278350515No Hit
GTACATGGAAGCAGTGGTATCAACG240.25773195876288657No Hit
TATCAACGCAGAGTACTTTTTTTTT210.22551546391752575No Hit
GGTATCAACGCAGAGTACTTTTTTT170.18256013745704466No Hit
TCCATGTACTCTGCGTTGATACCAC170.18256013745704466No Hit
GAATAGGACCGCGGTTCTATTTTGT160.1718213058419244No Hit
GTACATGGGTACCTGGTTGATCCTG160.1718213058419244No Hit
GCGCAAGACGGACCAGAGCGAAAGC150.1610824742268041No Hit
GAGTACTTTTTTTTTTTTTTTTTTT150.1610824742268041No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG150.1610824742268041No Hit
CTCCGAGGTCACCCCAACCGAAATT150.1610824742268041No Hit
ATCAGATACCGTCGTAGTTCCGACC140.15034364261168387No Hit
ACGCAGAGTACTTTTTTTTTTTTTT140.15034364261168387No Hit
GAACTACGACGGTATCTGATCGTCT130.13960481099656358No Hit
GTACTTTTTTTTTTTTTTTTTTTTT130.13960481099656358No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG130.13960481099656358No Hit
GTATCTGATCGTCTTCGAACCTCCG120.12886597938144329No Hit
ATCCTAGCCCTAGCCCTACACAAAT120.12886597938144329No Hit
CTCCACTCCTGGTGGTGCCCTTCCG120.12886597938144329No Hit
CTCCCGACCCGGGGAGGTAGTGACG120.12886597938144329No Hit
GTCCTATTCCATTATTCCTAGCTGC110.11812714776632302No Hit
GAGTACATGGAAGCAGTGGTATCAA110.11812714776632302No Hit
GTCCGTGTCCGGGCCGGGTGAGGTT100.10738831615120276No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)