##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062641_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9312
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.210266323024054	32.0	32.0	32.0	14.0	32.0
2	27.8349441580756	32.0	21.0	32.0	14.0	32.0
3	28.213917525773194	32.0	32.0	32.0	14.0	32.0
4	27.593642611683848	32.0	21.0	32.0	14.0	32.0
5	27.94737972508591	32.0	21.0	32.0	14.0	32.0
6	31.066365979381445	36.0	32.0	36.0	14.0	36.0
7	31.123603951890033	36.0	32.0	36.0	14.0	36.0
8	30.990442439862544	36.0	32.0	36.0	14.0	36.0
9	31.308741408934708	36.0	32.0	36.0	14.0	36.0
10	30.2858676975945	36.0	27.0	36.0	14.0	36.0
11	31.666559278350515	36.0	32.0	36.0	14.0	36.0
12	30.67310996563574	36.0	32.0	36.0	14.0	36.0
13	31.18567439862543	36.0	32.0	36.0	14.0	36.0
14	30.82678264604811	36.0	32.0	36.0	14.0	36.0
15	30.486898625429554	36.0	27.0	36.0	14.0	36.0
16	30.61855670103093	36.0	27.0	36.0	14.0	36.0
17	30.428157216494846	36.0	27.0	36.0	14.0	36.0
18	30.639712199312715	36.0	27.0	36.0	14.0	36.0
19	30.43825171821306	36.0	27.0	36.0	14.0	36.0
20	30.454252577319586	36.0	27.0	36.0	14.0	36.0
21	30.471219931271477	36.0	27.0	36.0	14.0	36.0
22	30.276417525773194	36.0	27.0	36.0	14.0	36.0
23	30.244952749140893	36.0	27.0	36.0	14.0	36.0
24	30.269329896907216	36.0	27.0	36.0	14.0	36.0
25	29.46166237113402	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	3.0
7	0.0
8	2.0
9	2.0
10	2.0
11	1.0
12	1.0
13	0.0
14	9.0
15	29.0
16	47.0
17	80.0
18	150.0
19	168.0
20	201.0
21	222.0
22	231.0
23	266.0
24	280.0
25	303.0
26	357.0
27	346.0
28	441.0
29	441.0
30	517.0
31	606.0
32	753.0
33	888.0
34	1404.0
35	1562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.33537436889032	19.873240949618648	11.61241809002041	26.17896659147062
2	15.64983888292159	20.225563909774436	38.6358754027927	25.48872180451128
3	18.921821305841924	23.883161512027492	27.74914089347079	29.445876288659793
4	11.640893470790378	15.560567010309278	36.67310996563574	36.1254295532646
5	14.469867869803416	35.524760983993986	34.55795466752605	15.447416478676548
6	33.42645320726335	36.56387665198238	15.869775437842485	14.139894702911787
7	29.805522724830773	30.332008165896635	21.747072096271623	18.115397013000965
8	27.353826311263973	32.41616509028375	19.937661220980225	20.292347377472055
9	26.57207352466946	13.930990003224766	19.45608943351607	40.0408470385897
10	15.775889880632327	25.486611463598237	32.33681040972148	26.40068824604796
11	36.936646229966655	22.146929116919438	22.28675916962461	18.629665483489298
12	23.70091447014524	24.367939752555138	28.98332436793975	22.947821409359868
13	28.85566788556679	19.488061948806195	25.790492579049257	25.86577758657776
14	22.856835538345702	19.99569753683984	25.567387329245996	31.58007959556846
15	23.531942353194236	27.984512798451277	23.166272316627232	25.31727253172725
16	24.77409638554217	26.861015490533564	24.182444061962137	24.182444061962137
17	23.34086264386361	26.255781434871462	26.16973217166828	24.233623749596646
18	23.668639053254438	24.550833781603014	28.63905325443787	23.14147391070468
19	24.088219472834858	25.788058095750404	26.369015599784827	23.75470683162991
20	25.158653329030873	24.4702592234054	26.804345487791757	23.56674195977197
21	25.099515868746636	23.92684238838085	26.54115115653577	24.43249058633674
22	23.773138183383555	25.85019371502368	26.130004304778303	24.246663796814463
23	23.383887275465202	24.911261697321716	26.804345487791757	24.90050553942132
24	22.92383820998279	25.54862306368331	27.527969018932875	23.999569707401033
25	22.874973100925327	25.478803529158593	27.028190230256076	24.618033139659996
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	1.0
2	4.0
3	7.0
4	7.0
5	7.0
6	14.5
7	22.0
8	22.0
9	22.0
10	29.5
11	37.0
12	37.0
13	37.0
14	41.0
15	45.0
16	45.0
17	45.0
18	60.0
19	75.0
20	75.0
21	75.0
22	134.5
23	194.0
24	194.0
25	194.0
26	275.5
27	357.0
28	357.0
29	357.0
30	439.5
31	522.0
32	522.0
33	522.0
34	587.0
35	652.0
36	652.0
37	652.0
38	716.5
39	781.0
40	781.0
41	781.0
42	878.0
43	975.0
44	975.0
45	975.0
46	1093.0
47	1211.0
48	1211.0
49	1211.0
50	1200.5
51	1190.0
52	1190.0
53	1190.0
54	1065.5
55	941.0
56	941.0
57	941.0
58	867.5
59	794.0
60	794.0
61	794.0
62	693.0
63	592.0
64	592.0
65	592.0
66	506.5
67	421.0
68	421.0
69	421.0
70	313.5
71	206.0
72	206.0
73	206.0
74	159.0
75	112.0
76	112.0
77	112.0
78	101.5
79	91.0
80	91.0
81	91.0
82	69.5
83	48.0
84	48.0
85	48.0
86	34.0
87	20.0
88	20.0
89	20.0
90	14.5
91	9.0
92	9.0
93	9.0
94	4.5
95	0.0
96	0.0
97	0.0
98	4.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03221649484536082
2	0.02147766323024055
3	0.0
4	0.0
5	0.03221649484536082
6	0.05369415807560138
7	0.05369415807560138
8	0.0859106529209622
9	0.09664948453608248
10	0.13960481099656358
11	0.1610824742268041
12	0.18256013745704466
13	0.15034364261168387
14	0.1610824742268041
15	0.15034364261168387
16	0.1718213058419244
17	0.1610824742268041
18	0.18256013745704466
19	0.18256013745704466
20	0.1610824742268041
21	0.18256013745704466
22	0.2147766323024055
23	0.1610824742268041
24	0.1718213058419244
25	0.19329896907216496
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9312.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.19243986254295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.60204081632652	77.9639175257732
2	4.030612244897959	6.786941580756014
3	1.2372448979591837	3.125
4	0.7397959183673469	2.491408934707904
5	0.4591836734693878	1.9329896907216495
6	0.2423469387755102	1.2242268041237114
7	0.14030612244897958	0.8268900343642612
8	0.17857142857142858	1.202749140893471
9	0.07653061224489796	0.5798969072164949
>10	0.29336734693877553	3.865979381443299
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	42	0.4510309278350515	No Hit
GTACATGGAAGCAGTGGTATCAACG	24	0.25773195876288657	No Hit
TATCAACGCAGAGTACTTTTTTTTT	21	0.22551546391752575	No Hit
GGTATCAACGCAGAGTACTTTTTTT	17	0.18256013745704466	No Hit
TCCATGTACTCTGCGTTGATACCAC	17	0.18256013745704466	No Hit
GAATAGGACCGCGGTTCTATTTTGT	16	0.1718213058419244	No Hit
GTACATGGGTACCTGGTTGATCCTG	16	0.1718213058419244	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	15	0.1610824742268041	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	15	0.1610824742268041	No Hit
ATCCTGTCCGTGTCCGGGCCGGGTG	15	0.1610824742268041	No Hit
CTCCGAGGTCACCCCAACCGAAATT	15	0.1610824742268041	No Hit
ATCAGATACCGTCGTAGTTCCGACC	14	0.15034364261168387	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	14	0.15034364261168387	No Hit
GAACTACGACGGTATCTGATCGTCT	13	0.13960481099656358	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	13	0.13960481099656358	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	13	0.13960481099656358	No Hit
GTATCTGATCGTCTTCGAACCTCCG	12	0.12886597938144329	No Hit
ATCCTAGCCCTAGCCCTACACAAAT	12	0.12886597938144329	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	12	0.12886597938144329	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	12	0.12886597938144329	No Hit
GTCCTATTCCATTATTCCTAGCTGC	11	0.11812714776632302	No Hit
GAGTACATGGAAGCAGTGGTATCAA	11	0.11812714776632302	No Hit
GTCCGTGTCCGGGCCGGGTGAGGTT	10	0.10738831615120276	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE