##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062641_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9312
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.956507731958762	32.0	32.0	32.0	21.0	32.0
2	29.954682130584192	32.0	32.0	32.0	21.0	32.0
3	30.737972508591064	32.0	32.0	32.0	32.0	32.0
4	31.22036082474227	32.0	32.0	32.0	32.0	32.0
5	30.944372852233677	32.0	32.0	32.0	32.0	32.0
6	34.454145189003434	36.0	36.0	36.0	32.0	36.0
7	34.58666237113402	36.0	36.0	36.0	32.0	36.0
8	34.520833333333336	36.0	36.0	36.0	32.0	36.0
9	34.735395189003434	36.0	36.0	36.0	32.0	36.0
10	34.45876288659794	36.0	36.0	36.0	32.0	36.0
11	34.92719072164948	36.0	36.0	36.0	32.0	36.0
12	34.68213058419244	36.0	36.0	36.0	32.0	36.0
13	34.746885738831615	36.0	36.0	36.0	32.0	36.0
14	34.70876288659794	36.0	36.0	36.0	32.0	36.0
15	34.523732817869416	36.0	36.0	36.0	32.0	36.0
16	34.66784793814433	36.0	36.0	36.0	32.0	36.0
17	34.575279209621996	36.0	36.0	36.0	32.0	36.0
18	34.642504295532646	36.0	36.0	36.0	32.0	36.0
19	34.490764604811	36.0	36.0	36.0	32.0	36.0
20	34.68094931271477	36.0	36.0	36.0	32.0	36.0
21	34.547895189003434	36.0	36.0	36.0	32.0	36.0
22	34.67353951890034	36.0	36.0	36.0	32.0	36.0
23	34.485502577319586	36.0	36.0	36.0	32.0	36.0
24	34.48786512027491	36.0	36.0	36.0	32.0	36.0
25	34.43191580756014	36.0	36.0	36.0	32.0	36.0
26	34.37403350515464	36.0	36.0	36.0	32.0	36.0
27	34.400236254295535	36.0	36.0	36.0	32.0	36.0
28	34.42697594501718	36.0	36.0	36.0	32.0	36.0
29	34.28447164948454	36.0	36.0	36.0	32.0	36.0
30	34.30358676975945	36.0	36.0	36.0	32.0	36.0
31	34.33140034364261	36.0	36.0	36.0	32.0	36.0
32	34.274806701030926	36.0	36.0	36.0	32.0	36.0
33	34.25064432989691	36.0	36.0	36.0	32.0	36.0
34	34.18642611683849	36.0	36.0	36.0	32.0	36.0
35	34.11694587628866	36.0	36.0	36.0	32.0	36.0
36	34.0224441580756	36.0	36.0	36.0	32.0	36.0
37	34.0941795532646	36.0	36.0	36.0	32.0	36.0
38	34.06325171821306	36.0	36.0	36.0	32.0	36.0
39	33.9022766323024	36.0	36.0	36.0	32.0	36.0
40	33.96542096219931	36.0	36.0	36.0	32.0	36.0
41	33.90259879725086	36.0	36.0	36.0	32.0	36.0
42	33.830004295532646	36.0	36.0	36.0	32.0	36.0
43	33.89637027491409	36.0	36.0	36.0	32.0	36.0
44	33.93889604810997	36.0	36.0	36.0	32.0	36.0
45	33.78339776632303	36.0	36.0	36.0	32.0	36.0
46	33.73453608247423	36.0	36.0	36.0	32.0	36.0
47	33.759020618556704	36.0	36.0	36.0	32.0	36.0
48	33.7948883161512	36.0	36.0	36.0	32.0	36.0
49	33.656464776632305	36.0	36.0	36.0	27.0	36.0
50	32.99645618556701	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	2.0
23	4.0
24	9.0
25	21.0
26	47.0
27	98.0
28	128.0
29	198.0
30	358.0
31	533.0
32	775.0
33	1228.0
34	2229.0
35	3681.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04123711340206	18.82517182130584	12.908075601374572	25.225515463917525
2	17.643900343642613	21.509879725085913	35.67439862542955	25.171821305841924
3	21.015893470790378	23.679123711340207	28.737113402061855	26.56786941580756
4	14.583333333333334	15.485395189003437	36.84493127147766	33.08634020618557
5	18.266752577319586	33.118556701030926	32.43127147766323	16.183419243986254
6	34.18170103092783	36.68384879725085	15.592783505154639	13.541666666666666
7	29.456615120274915	31.099656357388316	20.650773195876287	18.79295532646048
8	27.738402061855673	33.08634020618557	19.072164948453608	20.103092783505154
9	25.923539518900345	13.863831615120276	18.0090206185567	42.20360824742268
10	15.764604810996563	25.601374570446733	31.314432989690722	27.31958762886598
11	37.99398625429553	20.64003436426117	22.132731958762886	19.233247422680414
12	24.506013745704465	23.646907216494846	28.597508591065292	23.249570446735397
13	29.070017182130588	19.405068728522338	25.622852233676973	25.902061855670105
14	23.185137457044675	20.038659793814436	24.441580756013746	32.33462199312715
15	24.366408934707902	27.920962199312715	22.454896907216497	25.257731958762886
16	25.214776632302403	26.653780068728523	23.85094501718213	24.28049828178694
17	23.797250859106526	26.54639175257732	25.171821305841924	24.484536082474225
18	24.527491408934708	24.570446735395187	26.879295532646047	24.022766323024054
19	24.774484536082475	26.11683848797251	25.622852233676973	23.48582474226804
20	25.246993127147764	23.679123711340207	26.14905498281787	24.92482817869416
21	25.848367697594504	25.257731958762886	24.935567010309278	23.958333333333336
22	25.633591065292094	25.214776632302403	24.441580756013746	24.710051546391753
23	23.10996563573883	24.452319587628864	27.137027491408933	25.30068728522337
24	22.862972508591064	26.56786941580756	26.15979381443299	24.409364261168385
25	24.108676975945016	25.311426116838486	25.601374570446733	24.97852233676976
26	24.473797250859107	25.859106529209626	25.322164948453608	24.344931271477662
27	25.257731958762886	25.934278350515466	24.881872852233677	23.926116838487975
28	23.281786941580755	24.763745704467354	27.201460481099655	24.753006872852236
29	24.119415807560138	25.204037800687285	25.762457044673535	24.914089347079038
30	24.366408934707902	25.987972508591067	26.10609965635739	23.53951890034364
31	24.731529209621993	25.730240549828174	24.96778350515464	24.570446735395187
32	24.108676975945016	25.622852233676973	25.182560137457045	25.085910652920962
33	23.893900343642613	25.59063573883161	25.708762886597935	24.806701030927837
34	24.355670103092784	25.118127147766323	26.21348797250859	24.3127147766323
35	25.633591065292094	26.331615120274915	24.8926116838488	23.142182130584192
36	22.809278350515463	25.99871134020619	26.299398625429554	24.8926116838488
37	25.193298969072163	25.39733676975945	25.064432989690722	24.344931271477662
38	24.634879725085913	26.08462199312715	24.613402061855673	24.667096219931274
39	24.065721649484537	26.13831615120275	24.463058419243985	25.332903780068726
40	25.193298969072163	25.99871134020619	25.472508591065292	23.33548109965636
41	24.108676975945016	25.0536941580756	27.02963917525773	23.807989690721648
42	25.182560137457045	26.288659793814436	25.655068728522334	22.873711340206185
43	23.44286941580756	25.04295532646048	27.02963917525773	24.484536082474225
44	24.795962199312715	25.311426116838486	26.245704467353953	23.646907216494846
45	23.711340206185564	27.179982817869412	24.98926116838488	24.119415807560138
46	23.947594501718214	26.363831615120276	26.08462199312715	23.603951890034363
47	24.054982817869416	25.279209621993125	26.288659793814436	24.377147766323024
48	24.29123711340206	26.804123711340207	25.01073883161512	23.893900343642613
49	23.163659793814436	26.009450171821307	26.21348797250859	24.613402061855673
50	24.506013745704465	26.256443298969074	25.085910652920962	24.1516323024055
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.5
6	2.0
7	1.5
8	1.0
9	2.5
10	4.0
11	4.0
12	4.0
13	6.0
14	8.0
15	14.5
16	21.0
17	28.0
18	35.0
19	37.0
20	39.0
21	49.0
22	59.0
23	68.0
24	77.0
25	87.0
26	97.0
27	117.5
28	138.0
29	162.0
30	186.0
31	195.5
32	205.0
33	226.0
34	247.0
35	284.5
36	322.0
37	367.5
38	413.0
39	417.5
40	422.0
41	457.5
42	493.0
43	500.0
44	507.0
45	528.5
46	550.0
47	594.0
48	638.0
49	643.0
50	648.0
51	621.0
52	594.0
53	561.0
54	528.0
55	574.0
56	620.0
57	605.5
58	591.0
59	518.5
60	446.0
61	400.0
62	354.0
63	300.5
64	247.0
65	221.5
66	196.0
67	174.0
68	152.0
69	154.0
70	156.0
71	115.0
72	74.0
73	76.0
74	78.0
75	55.0
76	32.0
77	30.5
78	29.0
79	26.5
80	24.0
81	22.5
82	21.0
83	17.5
84	14.0
85	12.0
86	10.0
87	9.5
88	9.0
89	5.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	8.5
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	9312.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.58462199312714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.16899018062796	83.4192439862543
2	3.224633288883501	5.713058419243986
3	1.18802279064129	3.1572164948453607
4	0.43641653533761665	1.5463917525773196
5	0.3515577645775246	1.5571305841924399
6	0.2666989938174324	1.4175257731958764
7	0.08485877076009214	0.5262027491408935
8	0.1333494969087162	0.9450171821305842
9	0.024245363074312038	0.19329896907216496
>10	0.1212268153715602	1.524914089347079
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	23	0.2469931271477663	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	17	0.18256013745704466	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	16	0.1718213058419244	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	14	0.15034364261168387	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	14	0.15034364261168387	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	13	0.13960481099656358	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	13	0.13960481099656358	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	11	0.11812714776632302	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCTGAGTGTC	11	0.11812714776632302	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	10	0.10738831615120276	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.010738831615120275
11	0.0	0.0	0.0	0.0	0.010738831615120275
12	0.0	0.0	0.0	0.0	0.010738831615120275
13	0.0	0.0	0.0	0.0	0.010738831615120275
14	0.0	0.0	0.0	0.0	0.010738831615120275
15	0.0	0.0	0.0	0.0	0.010738831615120275
16	0.0	0.0	0.0	0.0	0.010738831615120275
17	0.0	0.0	0.0	0.0	0.010738831615120275
18	0.0	0.0	0.0	0.0	0.010738831615120275
19	0.0	0.0	0.0	0.0	0.010738831615120275
20	0.0	0.0	0.0	0.0	0.010738831615120275
21	0.0	0.0	0.0	0.0	0.010738831615120275
22	0.0	0.0	0.0	0.0	0.010738831615120275
23	0.0	0.0	0.0	0.010738831615120275	0.010738831615120275
24	0.0	0.0	0.0	0.010738831615120275	0.010738831615120275
25	0.0	0.0	0.0	0.010738831615120275	0.010738831615120275
26	0.0	0.0	0.0	0.02147766323024055	0.010738831615120275
27	0.0	0.0	0.0	0.02147766323024055	0.010738831615120275
28	0.0	0.0	0.0	0.02147766323024055	0.010738831615120275
29	0.0	0.0	0.0	0.0429553264604811	0.010738831615120275
30	0.0	0.0	0.0	0.06443298969072164	0.010738831615120275
31	0.0	0.0	0.0	0.10738831615120274	0.010738831615120275
32	0.0	0.0	0.0	0.13960481099656358	0.010738831615120275
33	0.0	0.0	0.0	0.19329896907216496	0.010738831615120275
34	0.0	0.0	0.0	0.23625429553264604	0.010738831615120275
35	0.0	0.0	0.0	0.26847079037800686	0.010738831615120275
36	0.0	0.0	0.0	0.40807560137457044	0.010738831615120275
37	0.0	0.0	0.0	0.5584192439862543	0.010738831615120275
38	0.0	0.0	0.0	0.7087628865979382	0.010738831615120275
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE