FastQCFastQC Report
Thu 2 Feb 2017
SRR4062640_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062640_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876736
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC43520.23189196562542627No Hit
GTCTTGCGCCGGTCCAAGAATTTCA40610.21638632178420406No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG39300.20940611785568133No Hit
GAATAGGACCGCGGTTCTATTTTGT39050.20807401786932206No Hit
GTATCAACGCAGAGTACTTTTTTTT37050.1974172179784477No Hit
GAACTACGACGGTATCTGATCGTCT35960.19160926203792117No Hit
GTATCTGATCGTCTTCGAACCTCCG35570.18953118605920066No Hit
GTCCTATTCCATTATTCCTAGCTGC34290.18271083412904107No Hit
GAATAACGCCGCCGCATCGCCAGTC33960.18095246214704677No Hit
GGGTAGGCACACGCTGAGCCAGTCA33780.1799933501568681No Hit
CTATTGGAGCTGGAATTACCGCGGC30500.16251619833583414No Hit
GATTAAGAGGGACGGCCGGGGGCAT30430.1621432103396535No Hit
ATCAGATACCGTCGTAGTTCCGACC28790.15340463442913654No Hit
GTACATGGGGAATAATTGCAATCCC28180.15015431046241987No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC28130.149887890465148No Hit
TCGTAGTTCCGACCATAAACGATGC27980.14908863047333243No Hit
GTGCATGGCCGTTCTTAGTTGGTGG27610.14711712249352066No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG27480.14642443050061382No Hit
GCTTTGAACACTCTAATTTTTTCAA26660.14205514254535534No Hit
CTCTTAATCATGGCCTCAGTTCCGA26550.14146901855135724No Hit
GTACATGGAAGCAGTGGTATCAACG26480.14109603055517667No Hit
TATCAACGCAGAGTACTTTTTTTTT26450.14093617855681354No Hit
CTTTAATATACGCTATTGGAGCTGG26060.13885810257809303No Hit
GTCCTGTATTGTTATTTTTCGTCAC25700.13693987859773565No Hit
TCCATGTACTCTGCGTTGATACCAC24200.12894727867957986No Hit
GAATAATGGAATAGGACCGCGGTTC23500.12521739871777382No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT23000.12255319874505526No Hit
ACCATACTCCCCCCGGAACCCAAAG22700.12095467876142409No Hit
GGATTGACAGATTGATAGCTCTTTC22050.11749121879688991No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA22050.11749121879688991No Hit
ATACAGGACTCTTTCGAGGCCCTGT22000.11722479879961807No Hit
GCCATGCACCACCACCCACGGAATC21740.1158394148138044No Hit
CATCTAAGGGCATCACAGACCTGTT21540.11477373482471696No Hit
GAATAATTGCAATCCCCGATCCCCA21410.11408104283181011No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21170.1128022268449052No Hit
CTCTAGATAACCTCGGGCCGATCGC20970.11173654685581777No Hit
GAGTATGGTTGCAAAGCTGAAACTT20700.11029787887054973No Hit
ATATTAAAGTTGCTGCAGTTAAAAA20650.11003145887327787No Hit
GTTCTATTTTGTTGGTTTTCGGAAC20330.10832637089073795No Hit
CTCCCGACCCGGGGAGGTAGTGACG19980.10646143090983495No Hit
GGTCGGGAGTGGGTAATTTGCGCGC19690.10491619492565817No Hit
GTTATTGCTCAATCTCGGGTGGCTG19590.10438335493111445No Hit
ACCTCGGGCCGATCGCACGCCCCCC19590.10438335493111445No Hit
CTTTAAATCCTTTAACGAGGATCCA19220.10241184695130269No Hit
GCCCCGGACATCTAAGGGCATCACA19210.10235856295184832No Hit
GTACATGGGTACCTGGTTGATCCTG18910.10076004296821715No Hit
CCATTATTCCTAGCTGCGGTATCCA18810.10022720297367343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAGT5650.013.95939818
ATCGCCA5750.013.881515516
CGCATCG5850.013.80481513
TCGCCAG5750.013.71662617
CAAGACG6700.013.4675574
ATTGTCG852.6993257E-713.4120476
CATCGCC5900.013.36718615
AAGACGG6700.013.18438155
GCACCGA655.452577E-513.1541236
GCATCGC6150.013.13211114
CGGTCCA8000.012.70719510
CACCGGT604.0919957E-412.6669347
AGAATTT8150.012.59224717
CGCAAGA6700.012.4718842
CCGCATC6400.012.47100812
AACCGCG2900.012.4485387
TGCACCG1301.9826984E-1012.4210195
TGTCGAT1001.4426405E-712.350268
AGGCCCG7200.012.27174810
GAATTTC8700.012.23308118
Produced by FastQC (version 0.11.5)