##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062640_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1876736
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.191680129757195	32.0	32.0	32.0	32.0	32.0
2	30.954091038910107	32.0	32.0	32.0	32.0	32.0
3	30.952024685411267	32.0	32.0	32.0	32.0	32.0
4	30.97844822074069	32.0	32.0	32.0	32.0	32.0
5	30.886258376244715	32.0	32.0	32.0	32.0	32.0
6	34.46416437900696	36.0	36.0	36.0	32.0	36.0
7	34.355648849918154	36.0	36.0	36.0	32.0	36.0
8	34.37205819039013	36.0	36.0	36.0	32.0	36.0
9	34.46663995362161	36.0	36.0	36.0	32.0	36.0
10	34.24895296941072	36.0	36.0	36.0	32.0	36.0
11	34.44677194874505	36.0	36.0	36.0	32.0	36.0
12	34.2735451336789	36.0	36.0	36.0	32.0	36.0
13	34.40472181489565	36.0	36.0	36.0	32.0	36.0
14	34.32210230954167	36.0	36.0	36.0	32.0	36.0
15	34.28382148581367	36.0	36.0	36.0	32.0	36.0
16	34.27032091887192	36.0	36.0	36.0	32.0	36.0
17	34.192044592313465	36.0	36.0	36.0	32.0	36.0
18	34.208691046583006	36.0	36.0	36.0	32.0	36.0
19	34.192906727424635	36.0	36.0	36.0	32.0	36.0
20	34.1851853430637	36.0	36.0	36.0	32.0	36.0
21	34.149512238269	36.0	36.0	36.0	32.0	36.0
22	34.121608473434726	36.0	36.0	36.0	32.0	36.0
23	34.107217530862094	36.0	36.0	36.0	32.0	36.0
24	34.0768264689333	36.0	36.0	36.0	32.0	36.0
25	33.60319885162324	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	8.0
4	76.0
5	187.0
6	294.0
7	78.0
8	279.0
9	359.0
10	232.0
11	57.0
12	132.0
13	109.0
14	417.0
15	546.0
16	798.0
17	1142.0
18	1526.0
19	1970.0
20	2668.0
21	3682.0
22	4988.0
23	6859.0
24	9366.0
25	12973.0
26	18296.0
27	24144.0
28	33101.0
29	45287.0
30	59591.0
31	83426.0
32	123778.0
33	185848.0
34	438964.0
35	815554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.47880121049157	17.968254171256103	11.055993494593661	25.496951123658672
2	17.4635272878604	20.171132272628693	35.98845192859507	26.376888510915837
3	18.83791305849121	23.962814411493728	28.16052723989298	29.038745290122087
4	12.206906034066527	14.970588015855505	36.11310956989969	36.70939638017827
5	14.713603367008032	36.31537343417443	33.007584403068726	15.96343879574881
6	34.38461874711471	35.51474656883598	16.67306361660094	13.427571067448374
7	30.753649075567523	30.438368946533544	20.67550887108599	18.13247310681294
8	28.55042358531571	31.453042961177346	19.799100297856338	20.197433155650604
9	26.994110763723327	14.337106241993103	19.31996974846209	39.34881324582148
10	16.301170214582015	25.444645265368138	30.54006128476075	27.714123235289094
11	37.9234370990621	21.344606673420426	21.89707075605318	18.83488547146429
12	25.00542879475421	24.316305325020743	27.48858886016652	23.18967702005853
13	28.284807047194693	18.84074671583314	26.181467824127424	26.69297841284474
14	23.422692864379407	20.673618887619753	24.440504840715466	31.46318340728537
15	25.198308761006338	27.33305522985679	22.17724082231855	25.29139518681832
16	26.445949463783514	25.88034029369215	23.420583202106457	24.253127040417876
17	23.89659554444934	26.227815875366645	24.9904770847182	24.88511149546581
18	25.465716887882746	23.997404843829116	26.544008545234625	23.992869723053513
19	25.287915012450306	25.32990511943171	25.339242182610544	24.042937685507436
20	25.288582139677118	24.18701648453951	24.85943674400069	25.664964631782688
21	27.425943996956715	24.105701836054276	24.049253824512842	24.41910034247617
22	25.641861676354004	24.377402712169758	25.00453593635601	24.976199675120228
23	23.563924610210584	24.005830900678866	25.98667237946955	26.443572109640996
24	24.24009437561259	25.610856396791615	25.12724007356688	25.02180915402892
25	24.459159593204568	24.719902638856208	25.17618963014183	25.64474813779739
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	288.0
1	288.0
2	655.0
3	1022.0
4	1022.0
5	1022.0
6	2801.0
7	4580.0
8	4580.0
9	4580.0
10	4859.5
11	5139.0
12	5139.0
13	5139.0
14	5721.0
15	6303.0
16	6303.0
17	6303.0
18	9867.0
19	13431.0
20	13431.0
21	13431.0
22	22003.5
23	30576.0
24	30576.0
25	30576.0
26	45665.5
27	60755.0
28	60755.0
29	60755.0
30	71339.5
31	81924.0
32	81924.0
33	81924.0
34	102834.5
35	123745.0
36	123745.0
37	123745.0
38	135678.5
39	147612.0
40	147612.0
41	147612.0
42	165896.0
43	184180.0
44	184180.0
45	184180.0
46	214965.5
47	245751.0
48	245751.0
49	245751.0
50	242827.5
51	239904.0
52	239904.0
53	239904.0
54	217167.0
55	194430.0
56	194430.0
57	194430.0
58	183082.5
59	171735.0
60	171735.0
61	171735.0
62	156794.5
63	141854.0
64	141854.0
65	141854.0
66	119738.0
67	97622.0
68	97622.0
69	97622.0
70	74098.0
71	50574.0
72	50574.0
73	50574.0
74	41369.5
75	32165.0
76	32165.0
77	32165.0
78	28709.5
79	25254.0
80	25254.0
81	25254.0
82	17697.5
83	10141.0
84	10141.0
85	10141.0
86	7542.0
87	4943.0
88	4943.0
89	4943.0
90	3348.0
91	1753.0
92	1753.0
93	1753.0
94	995.0
95	237.0
96	237.0
97	237.0
98	527.5
99	818.0
100	818.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007619611921975173
2	0.003143755967807939
3	0.005061979948165325
4	0.015452359841767837
5	0.03458131564588733
6	0.04486512754058109
7	0.06719112331196289
8	0.08903756308825536
9	0.09537835902332562
10	0.11765107079525303
11	0.11855689878597735
12	0.13166476265175284
13	0.12191379075160277
14	0.1237787307325058
15	0.11370805483562953
16	0.11296207884326831
17	0.12308603873959897
18	0.1318778986495703
19	0.13241073864411404
20	0.12324589073796208
21	0.13027937866593917
22	0.14983460646569363
23	0.1376325705906425
24	0.13443553062338018
25	0.13198446664847907
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1876736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.013216383505466
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00967463199716	32.81454098455967
2	12.25400396451675	10.051522323221187
3	3.3501900188709475	4.122062045094433
4	1.346943806008004	2.2096999108891464
5	0.698572101182185	1.4325344372632514
6	0.4264800906988468	1.0494792145853304
7	0.2963406961503241	0.8507719566116321
8	0.21296953501594823	0.6987652498162902
9	0.17238205698980738	0.6362948343561066
>10	0.9222615401316435	7.090335923045821
>50	0.11206001575966319	3.2612559284625897
>100	0.14719469202857882	13.2001756544252
>500	0.03156945078569737	8.875052663193816
>1k	0.019357399864481102	13.70750887447561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	4352	0.23189196562542627	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	4061	0.21638632178420406	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3930	0.20940611785568133	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3905	0.20807401786932206	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3705	0.1974172179784477	No Hit
GAACTACGACGGTATCTGATCGTCT	3596	0.19160926203792117	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3557	0.18953118605920066	No Hit
GTCCTATTCCATTATTCCTAGCTGC	3429	0.18271083412904107	No Hit
GAATAACGCCGCCGCATCGCCAGTC	3396	0.18095246214704677	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	3378	0.1799933501568681	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3050	0.16251619833583414	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3043	0.1621432103396535	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2879	0.15340463442913654	No Hit
GTACATGGGGAATAATTGCAATCCC	2818	0.15015431046241987	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2813	0.149887890465148	No Hit
TCGTAGTTCCGACCATAAACGATGC	2798	0.14908863047333243	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	2761	0.14711712249352066	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	2748	0.14642443050061382	No Hit
GCTTTGAACACTCTAATTTTTTCAA	2666	0.14205514254535534	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2655	0.14146901855135724	No Hit
GTACATGGAAGCAGTGGTATCAACG	2648	0.14109603055517667	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2645	0.14093617855681354	No Hit
CTTTAATATACGCTATTGGAGCTGG	2606	0.13885810257809303	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	2570	0.13693987859773565	No Hit
TCCATGTACTCTGCGTTGATACCAC	2420	0.12894727867957986	No Hit
GAATAATGGAATAGGACCGCGGTTC	2350	0.12521739871777382	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	2300	0.12255319874505526	No Hit
ACCATACTCCCCCCGGAACCCAAAG	2270	0.12095467876142409	No Hit
GGATTGACAGATTGATAGCTCTTTC	2205	0.11749121879688991	No Hit
ACCCCCGAGCGGTCGGCGTCCCCCA	2205	0.11749121879688991	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	2200	0.11722479879961807	No Hit
GCCATGCACCACCACCCACGGAATC	2174	0.1158394148138044	No Hit
CATCTAAGGGCATCACAGACCTGTT	2154	0.11477373482471696	No Hit
GAATAATTGCAATCCCCGATCCCCA	2141	0.11408104283181011	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2117	0.1128022268449052	No Hit
CTCTAGATAACCTCGGGCCGATCGC	2097	0.11173654685581777	No Hit
GAGTATGGTTGCAAAGCTGAAACTT	2070	0.11029787887054973	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	2065	0.11003145887327787	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	2033	0.10832637089073795	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	1998	0.10646143090983495	No Hit
GGTCGGGAGTGGGTAATTTGCGCGC	1969	0.10491619492565817	No Hit
GTTATTGCTCAATCTCGGGTGGCTG	1959	0.10438335493111445	No Hit
ACCTCGGGCCGATCGCACGCCCCCC	1959	0.10438335493111445	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1922	0.10241184695130269	No Hit
GCCCCGGACATCTAAGGGCATCACA	1921	0.10235856295184832	No Hit
GTACATGGGTACCTGGTTGATCCTG	1891	0.10076004296821715	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1881	0.10022720297367343	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.065679989087437E-4	0.0
8	0.0	0.0	0.0	1.065679989087437E-4	5.328399945437185E-5
9	0.0	0.0	0.0	1.065679989087437E-4	5.328399945437185E-5
10	0.0	0.0	0.0	1.065679989087437E-4	5.328399945437185E-5
11	0.0	0.0	0.0	1.065679989087437E-4	5.328399945437185E-5
12	0.0	0.0	0.0	1.065679989087437E-4	2.131359978174874E-4
13	0.0	0.0	0.0	1.065679989087437E-4	2.664199972718592E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCAGT	565	0.0	13.959398	18
ATCGCCA	575	0.0	13.8815155	16
CGCATCG	585	0.0	13.804815	13
TCGCCAG	575	0.0	13.716626	17
CAAGACG	670	0.0	13.467557	4
ATTGTCG	85	2.6993257E-7	13.412047	6
CATCGCC	590	0.0	13.367186	15
AAGACGG	670	0.0	13.1843815	5
GCACCGA	65	5.452577E-5	13.154123	6
GCATCGC	615	0.0	13.132111	14
CGGTCCA	800	0.0	12.707195	10
CACCGGT	60	4.0919957E-4	12.666934	7
AGAATTT	815	0.0	12.592247	17
CGCAAGA	670	0.0	12.471884	2
CCGCATC	640	0.0	12.471008	12
AACCGCG	290	0.0	12.448538	7
TGCACCG	130	1.9826984E-10	12.421019	5
TGTCGAT	100	1.4426405E-7	12.35026	8
AGGCCCG	720	0.0	12.271748	10
GAATTTC	870	0.0	12.233081	18
>>END_MODULE