FastQCFastQC Report
Thu 2 Feb 2017
SRR4062639_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062639_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142968
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16450.1439235394166766No Hit
GCGCAAGACGGACCAGAGCGAAAGC14720.12878750761176166No Hit
GTACATGGAAGCAGTGGTATCAACG14630.12800008399185278No Hit
TCCATGTACTCTGCGTTGATACCAC13010.11382645883349315No Hit
GAATAGGACCGCGGTTCTATTTTGT12610.11032679830056484No Hit
GAATAACGCCGCCGCATCGCCAGTC12600.11023930678724164No Hit
GAACTACGACGGTATCTGATCGTCT12480.10918940862736314No Hit
GTATCTGATCGTCTTCGAACCTCCG12290.1075270698742222No Hit
GTCTTGCGCCGGTCCAAGAATTTCA12290.1075270698742222No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12230.10700212079428296No Hit
GATTAAGAGGGACGGCCGGGGGCAT12130.10612720566105088No Hit
GTCCTATTCCATTATTCCTAGCTGC11870.10385242631464747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGC508.749321E-515.19212151
GTATTAT400.00529615614.2426141
GCGTTAT1152.5465852E-1114.0361991
GTCTTAA1051.378794E-913.5643941
GTTTAAA500.001504991113.2931061
CGTTATT1153.8016879E-1013.210542
TTATACC802.0028401E-613.0597314
TTAGGGT1102.752131E-912.9517984
CCGACCT751.4763342E-512.66786419
GCGCCGA1352.7284841E-1112.66786319
ACAGTTC1155.313268E-912.3919338
AACTTAT701.0898951E-412.2149067
TATATCT1103.8207872E-812.0883464
GTATTAA1103.8369762E-812.0846411
CGGTCCA2300.011.979918510
ACGGTAT2400.011.8766429
GCATCGC2250.011.82489314
GTCCTAA1850.011.8046881
GTTATTC1459.822543E-1111.7885393
TTACCCC658.0348394E-411.6898294
Produced by FastQC (version 0.11.5)