##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062639_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1142968
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.094496083879864	32.0	32.0	32.0	32.0	32.0
2	30.728735187686794	32.0	32.0	32.0	32.0	32.0
3	30.755238991817794	32.0	32.0	32.0	32.0	32.0
4	30.65865361059977	32.0	32.0	32.0	32.0	32.0
5	30.647389953174542	32.0	32.0	32.0	32.0	32.0
6	34.07611849150632	36.0	36.0	36.0	32.0	36.0
7	33.99464026989382	36.0	36.0	36.0	32.0	36.0
8	33.91296781712174	36.0	36.0	36.0	32.0	36.0
9	34.16454791385236	36.0	36.0	36.0	32.0	36.0
10	33.745054979666975	36.0	36.0	36.0	32.0	36.0
11	34.14915115733774	36.0	36.0	36.0	32.0	36.0
12	33.903390996073384	36.0	36.0	36.0	32.0	36.0
13	34.03200177082823	36.0	36.0	36.0	32.0	36.0
14	33.90032091887087	36.0	36.0	36.0	32.0	36.0
15	33.87724415731674	36.0	36.0	36.0	32.0	36.0
16	33.92150611390695	36.0	36.0	36.0	32.0	36.0
17	33.7762483289121	36.0	36.0	36.0	32.0	36.0
18	33.86945478788557	36.0	36.0	36.0	32.0	36.0
19	33.8245908896837	36.0	36.0	36.0	32.0	36.0
20	33.736230585633194	36.0	36.0	36.0	32.0	36.0
21	33.70622449622387	36.0	36.0	36.0	32.0	36.0
22	33.71450556795991	36.0	36.0	36.0	32.0	36.0
23	33.62339453073052	36.0	36.0	36.0	27.0	36.0
24	33.60550689083159	36.0	36.0	36.0	27.0	36.0
25	32.956364482645185	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	7.0
4	49.0
5	129.0
6	198.0
7	63.0
8	165.0
9	192.0
10	139.0
11	53.0
12	89.0
13	53.0
14	250.0
15	475.0
16	780.0
17	1047.0
18	1298.0
19	1592.0
20	2155.0
21	2723.0
22	3641.0
23	5020.0
24	6736.0
25	9269.0
26	12830.0
27	17262.0
28	23942.0
29	32947.0
30	45414.0
31	65480.0
32	100363.0
33	150722.0
34	309977.0
35	347906.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.338790995906756	17.507599369300472	10.750241937318547	26.403367697474227
2	16.98892405201732	20.375229564092894	36.576789243757474	26.059057140132307
3	19.34314808041956	24.02839778599465	28.33373873266918	28.29471540091661
4	12.720176762704819	15.210955787447222	35.89332983308176	36.175537616766206
5	14.643453875649765	36.43944890773204	33.63619893026973	15.280898286348465
6	34.16230893528966	35.42988793904836	16.729136722764252	13.678666402897724
7	29.6087140384378	30.251620398946905	21.355557948688315	18.78410761392698
8	27.727658039182717	32.96071920219418	19.97448184305195	19.337140915571155
9	27.307130445749202	13.841906870507126	18.517736782366214	40.33322590137746
10	15.572936878001862	26.735944736387978	32.039019501867145	25.65209888374301
11	37.88538691988297	20.876416895881146	22.29392596226283	18.944270221973056
12	24.414470767780514	23.45533647671462	29.242557247561425	22.887635507943433
13	29.549275071590568	19.518711658860106	25.294224312148017	25.637788957401312
14	23.13632943250017	19.947597610619237	24.88693015029552	32.02914280658508
15	24.945517739143842	27.823179661824575	22.14642589361419	25.084876705417393
16	25.59406343859133	25.636107074630083	24.272842176073492	24.496987310705098
17	23.88072906674936	25.843641514441966	25.518212214942764	24.75741720386591
18	24.72916787124107	24.63875908687128	26.839494621903803	23.792578419983844
19	25.496995076481	24.998072643807053	25.046957405428138	24.45797487428381
20	25.37697950373782	24.550489061593904	25.2560937792907	24.816437655377573
21	26.443607896182353	24.381254582934357	24.113527989956747	25.06160953092654
22	25.439342055917535	24.879868775695467	24.908171026206308	24.772618142180693
23	24.125036579104613	24.570819774970605	25.45520176771261	25.848941878212173
24	24.715621375180035	25.23006108095417	25.27491685911431	24.779400684751486
25	24.695258169814398	24.732754074175308	25.32077395751749	25.251213798492806
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	83.0
1	83.0
2	270.5
3	458.0
4	458.0
5	458.0
6	1090.0
7	1722.0
8	1722.0
9	1722.0
10	2053.0
11	2384.0
12	2384.0
13	2384.0
14	2937.0
15	3490.0
16	3490.0
17	3490.0
18	5493.0
19	7496.0
20	7496.0
21	7496.0
22	12486.0
23	17476.0
24	17476.0
25	17476.0
26	26424.0
27	35372.0
28	35372.0
29	35372.0
30	44661.0
31	53950.0
32	53950.0
33	53950.0
34	65050.5
35	76151.0
36	76151.0
37	76151.0
38	85053.5
39	93956.0
40	93956.0
41	93956.0
42	106169.0
43	118382.0
44	118382.0
45	118382.0
46	132983.0
47	147584.0
48	147584.0
49	147584.0
50	147543.5
51	147503.0
52	147503.0
53	147503.0
54	136863.5
55	126224.0
56	126224.0
57	126224.0
58	116471.0
59	106718.0
60	106718.0
61	106718.0
62	95097.5
63	83477.0
64	83477.0
65	83477.0
66	69667.5
67	55858.0
68	55858.0
69	55858.0
70	42566.0
71	29274.0
72	29274.0
73	29274.0
74	22989.5
75	16705.0
76	16705.0
77	16705.0
78	13862.0
79	11019.0
80	11019.0
81	11019.0
82	7733.0
83	4447.0
84	4447.0
85	4447.0
86	3241.5
87	2036.0
88	2036.0
89	2036.0
90	1346.5
91	657.0
92	657.0
93	657.0
94	380.0
95	103.0
96	103.0
97	103.0
98	273.0
99	443.0
100	443.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009624066465552842
2	0.0035871520462515136
3	0.0054244738260388736
4	0.016885862071379074
5	0.03823379132224174
6	0.04847029838105704
7	0.0709556173051214
8	0.09064120780284313
9	0.10149015545492088
10	0.12117574595264259
11	0.12143822049261223
12	0.13316208327792206
13	0.12310055924575317
14	0.12397547437898525
15	0.11478886548004844
16	0.1134764927802003
17	0.12240062713916751
18	0.13001238879828655
19	0.13193720209139714
20	0.12196316957255146
21	0.1316747275514275
22	0.15013543686262434
23	0.14051137039707148
24	0.13403699841115413
25	0.13211218511804357
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1142968.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.23177855766516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.25367477702503	46.25270425615879
2	11.10150835702851	12.485151191769928
3	2.906137513330523	4.902518433231769
4	1.1979031464052163	2.6944089784875382
5	0.611006332319258	1.7178986388153838
6	0.37841495555935983	1.2767367590353573
7	0.25622412083739765	1.0085556616842688
8	0.18574761949568255	0.8355935205675735
9	0.13543650744040167	0.6854252125511013
>10	0.7765812142467877	8.242711964313404
>50	0.09277634395378802	3.6735473437024977
>100	0.0927429554942201	10.94022984644569
>500	0.009508099588055652	3.635494686178474
>1k	0.0023380572757513898	1.649023507058243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1645	0.1439235394166766	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1472	0.12878750761176166	No Hit
GTACATGGAAGCAGTGGTATCAACG	1463	0.12800008399185278	No Hit
TCCATGTACTCTGCGTTGATACCAC	1301	0.11382645883349315	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1261	0.11032679830056484	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1260	0.11023930678724164	No Hit
GAACTACGACGGTATCTGATCGTCT	1248	0.10918940862736314	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1229	0.1075270698742222	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1229	0.1075270698742222	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1223	0.10700212079428296	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1213	0.10612720566105088	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1187	0.10385242631464747	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.749151332320765E-5
2	0.0	0.0	0.0	0.0	8.749151332320765E-5
3	0.0	0.0	0.0	0.0	8.749151332320765E-5
4	0.0	0.0	0.0	0.0	8.749151332320765E-5
5	0.0	0.0	0.0	0.0	3.499660532928306E-4
6	0.0	0.0	0.0	0.0	5.249490799392459E-4
7	0.0	0.0	0.0	0.0	5.249490799392459E-4
8	0.0	0.0	0.0	0.0	5.249490799392459E-4
9	0.0	0.0	0.0	0.0	5.249490799392459E-4
10	0.0	0.0	0.0	0.0	6.124405932624535E-4
11	0.0	0.0	0.0	0.0	6.124405932624535E-4
12	0.0	0.0	0.0	0.0	9.624066465552841E-4
13	0.0	0.0	0.0	0.0	0.0010498981598784918
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGC	50	8.749321E-5	15.1921215	1
GTATTAT	40	0.005296156	14.242614	1
GCGTTAT	115	2.5465852E-11	14.036199	1
GTCTTAA	105	1.378794E-9	13.564394	1
GTTTAAA	50	0.0015049911	13.293106	1
CGTTATT	115	3.8016879E-10	13.21054	2
TTATACC	80	2.0028401E-6	13.059731	4
TTAGGGT	110	2.752131E-9	12.951798	4
CCGACCT	75	1.4763342E-5	12.667864	19
GCGCCGA	135	2.7284841E-11	12.667863	19
ACAGTTC	115	5.313268E-9	12.391933	8
AACTTAT	70	1.0898951E-4	12.214906	7
TATATCT	110	3.8207872E-8	12.088346	4
GTATTAA	110	3.8369762E-8	12.084641	1
CGGTCCA	230	0.0	11.9799185	10
ACGGTAT	240	0.0	11.876642	9
GCATCGC	225	0.0	11.824893	14
GTCCTAA	185	0.0	11.804688	1
GTTATTC	145	9.822543E-11	11.788539	3
TTACCCC	65	8.0348394E-4	11.689829	4
>>END_MODULE