FastQCFastQC Report
Thu 2 Feb 2017
SRR4062639_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062639_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142968
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG14420.12616276221206543No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC13580.118813475092916No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT13020.11391395034681635No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12710.11120171343379692No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA11990.10490232447452597No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11640.1018401215082137No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11440.10009029124174953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG550.004483541519.999944
GATTTCG3350.019.70143341
GTCCTAC6600.019.669191
GTCCTAA3500.019.4882141
TCCTACA6650.019.1879122
TTAGAAC1851.8189894E-1219.0269723
TTAACGG1404.0799932E-918.85708635
TACACGG708.123117E-418.8570865
TAGGACG5700.018.526264
GACTGCG951.5970269E-518.526267
ATAACCG600.007411988518.3332795
ATAACGC4100.018.2438493
TAACGCC4150.018.0240424
CGTGATC750.001290838117.59994738
ATAGCAC1254.8520633E-717.5999473
ACGTGAT750.001290838117.59994737
AATAACG4400.017.4999492
TAGCACT1903.274181E-1117.368374
ATTTCGT3550.017.35206242
GATATAC3050.017.3136961
Produced by FastQC (version 0.11.5)