##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062639_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1142968
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.163567133988003	32.0	32.0	32.0	32.0	32.0
2	31.127851348419203	32.0	32.0	32.0	32.0	32.0
3	31.307908882838365	32.0	32.0	32.0	32.0	32.0
4	31.477822651202832	32.0	32.0	32.0	32.0	32.0
5	31.34062195966991	32.0	32.0	32.0	32.0	32.0
6	34.93829486039854	36.0	36.0	36.0	36.0	36.0
7	34.954360926990084	36.0	36.0	36.0	36.0	36.0
8	34.876218756780595	36.0	36.0	36.0	32.0	36.0
9	35.07289792890089	36.0	36.0	36.0	36.0	36.0
10	34.87424757298542	36.0	36.0	36.0	32.0	36.0
11	35.072590833689134	36.0	36.0	36.0	36.0	36.0
12	34.952488608604966	36.0	36.0	36.0	32.0	36.0
13	35.017444932841514	36.0	36.0	36.0	36.0	36.0
14	34.97234480755367	36.0	36.0	36.0	32.0	36.0
15	34.90738935823225	36.0	36.0	36.0	32.0	36.0
16	34.934936061202066	36.0	36.0	36.0	32.0	36.0
17	34.9080779164421	36.0	36.0	36.0	32.0	36.0
18	34.92991054867678	36.0	36.0	36.0	32.0	36.0
19	34.90367796823708	36.0	36.0	36.0	32.0	36.0
20	34.90104447368605	36.0	36.0	36.0	32.0	36.0
21	34.89673026716409	36.0	36.0	36.0	32.0	36.0
22	34.8798864010191	36.0	36.0	36.0	32.0	36.0
23	34.82891472027213	36.0	36.0	36.0	32.0	36.0
24	34.79874239698749	36.0	36.0	36.0	32.0	36.0
25	34.76957622610607	36.0	36.0	36.0	32.0	36.0
26	34.71153085650692	36.0	36.0	36.0	32.0	36.0
27	34.70617287623101	36.0	36.0	36.0	32.0	36.0
28	34.67167672235793	36.0	36.0	36.0	32.0	36.0
29	34.65314601983608	36.0	36.0	36.0	32.0	36.0
30	34.634726431536144	36.0	36.0	36.0	32.0	36.0
31	34.63788400025197	36.0	36.0	36.0	32.0	36.0
32	34.60047175423984	36.0	36.0	36.0	32.0	36.0
33	34.57961990186952	36.0	36.0	36.0	32.0	36.0
34	34.566693905691146	36.0	36.0	36.0	32.0	36.0
35	34.542437758537424	36.0	36.0	36.0	32.0	36.0
36	34.50237451967159	36.0	36.0	36.0	32.0	36.0
37	34.49795007384284	36.0	36.0	36.0	32.0	36.0
38	34.45085339222096	36.0	36.0	36.0	32.0	36.0
39	34.43553800281373	36.0	36.0	36.0	32.0	36.0
40	34.4328730113179	36.0	36.0	36.0	32.0	36.0
41	34.398963925499224	36.0	36.0	36.0	32.0	36.0
42	34.36935767230579	36.0	36.0	36.0	32.0	36.0
43	34.36486760784204	36.0	36.0	36.0	32.0	36.0
44	34.32420242736455	36.0	36.0	36.0	32.0	36.0
45	34.27599285369319	36.0	36.0	36.0	32.0	36.0
46	34.28903083900862	36.0	36.0	36.0	32.0	36.0
47	34.27124381435001	36.0	36.0	36.0	32.0	36.0
48	34.237660196960896	36.0	36.0	36.0	32.0	36.0
49	34.223393830798415	36.0	36.0	36.0	32.0	36.0
50	33.61928592926486	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	19.0
22	78.0
23	273.0
24	724.0
25	1774.0
26	3632.0
27	6920.0
28	11859.0
29	19269.0
30	29508.0
31	44380.0
32	68278.0
33	115505.0
34	244866.0
35	595879.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.81697419621864	17.796987619190123	11.084714350591565	26.301323833999668
2	16.559281282045	20.670928250697333	36.482915749428656	26.286874717829008
3	19.09321490409559	24.1705535009506	28.715327994568497	28.020903600385317
4	12.786568267619744	15.73617739825804	36.20784538459183	35.26940894953039
5	14.821062356951376	36.22489868482757	33.37101301173786	15.583025946483192
6	34.48087302608037	35.601990258686385	16.465042297809124	13.452094417424126
7	29.754201342469784	30.306447774565864	21.148798566539046	18.79055231642531
8	27.954675896438047	33.06759244353298	19.67404161796306	19.30369004206592
9	27.49114585885169	13.571246089129355	18.367793324047568	40.56981472797139
10	15.711026030475045	26.721745490687404	31.843848646681273	25.72337983215628
11	38.21568057898384	20.720527608821946	22.19274730351156	18.871044508682658
12	24.598763919899767	23.278429492339242	29.18611894646219	22.936687641298796
13	29.861290954777388	19.37823281141729	25.104902324474526	25.65557390933079
14	23.24369536154993	19.923567413960846	24.767535049100236	32.06520217538898
15	25.14856058962281	27.688001763828908	21.95459540424579	25.2088422423025
16	25.8568918814875	25.522761792106163	24.115723274842342	24.504623051563996
17	24.150894863198268	25.74350288022062	25.297733619838876	24.807868636742235
18	25.072967922111555	24.513197219869674	26.54300032896809	23.870834529050683
19	25.761613623478524	24.90559665712426	24.848289715897558	24.484500003499658
20	25.64743719859174	24.484325020473012	24.952579599778822	24.915658181156427
21	26.79121375226603	24.289568911815554	23.92122964072485	24.997987695193565
22	25.706756444624872	24.938493466133785	24.569279279909846	24.785470809331496
23	24.40129557432929	24.443903941317693	25.279710368094293	25.875090116258725
24	24.95537932820516	25.09659063070882	25.063343855646004	24.884686185440014
25	25.0878414793765	24.625098865410056	25.1157512721266	25.17130838308684
26	24.332877210910542	25.557583414408803	25.598791917184034	24.510747457496624
27	25.351803375072617	24.933068992307746	24.79054531710424	24.924582315515394
28	24.208726753504912	25.032021893876294	25.306920228737813	25.452331123880985
29	24.40488272637554	24.952404616752176	25.282422605007316	25.36029005186497
30	24.330514940050815	25.197905803137093	25.589167850718482	24.88241140609361
31	25.00988654100552	24.926419637295183	24.540757046566483	25.52293677513281
32	24.705503566154082	25.088453919969762	24.639184999055093	25.566857514821063
33	24.213276312197717	24.64268465958802	25.690308040120108	25.453730988094154
34	25.081192124363938	24.818455109854344	25.685321023860684	24.415031741921034
35	25.549446703669744	24.68739282289618	25.448918954861377	24.3142415185727
36	24.430955197345856	25.412260010778954	25.053807280693775	25.102977511181418
37	25.305345381497997	25.021347929250865	24.750561695515536	24.922744993735606
38	24.557467925611213	24.836128395545632	25.253550405610653	25.352853273232494
39	25.170170993413638	24.33095239761743	24.94243058423333	25.556446024735603
40	25.723817289722895	24.577328499135582	25.445681768868422	24.2531724422731
41	24.40287042156911	24.709703158793598	26.077807952628596	24.8096184670087
42	25.743852846273914	25.351540900532648	25.237539458672508	23.66706679452093
43	24.480737868426765	24.33970154894975	25.71655549411707	25.463005088506414
44	24.06331585836174	24.93508129711418	25.392049471201293	25.609553373322786
45	24.27145816855765	25.25722504917023	25.40088611404694	25.07043066822518
46	24.115261622184406	24.525685388441467	25.739370846876326	25.6196821424978
47	24.636756791753392	24.243657078463333	26.077743276927507	25.041842852855765
48	24.920470214389205	25.251888066857514	25.034996605329283	24.792645113423998
49	24.060778604475367	25.028784707883332	25.586630596832105	25.323806090809192
50	23.91766341460427	25.669944976539128	25.11866046876244	25.29373114009416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	8.0
2	13.0
3	30.5
4	48.0
5	81.0
6	114.0
7	101.0
8	88.0
9	132.5
10	177.0
11	257.0
12	337.0
13	563.0
14	789.0
15	1118.5
16	1448.0
17	1820.0
18	2192.0
19	2493.0
20	2794.0
21	3466.5
22	4139.0
23	4719.5
24	5300.0
25	6685.0
26	8070.0
27	10336.5
28	12603.0
29	14822.5
30	17042.0
31	18971.0
32	20900.0
33	24527.5
34	28155.0
35	32740.5
36	37326.0
37	42431.0
38	47536.0
39	49774.5
40	52013.0
41	55220.5
42	58428.0
43	59027.0
44	59626.0
45	65540.5
46	71455.0
47	76520.0
48	81585.0
49	83677.0
50	85769.0
51	81646.0
52	77523.0
53	76482.0
54	75441.0
55	76466.0
56	77491.0
57	76264.0
58	75037.0
59	68749.5
60	62462.0
61	55059.5
62	47657.0
63	41605.0
64	35553.0
65	30729.0
66	25905.0
67	22928.0
68	19951.0
69	18603.0
70	17255.0
71	13465.0
72	9675.0
73	8869.0
74	8063.0
75	6013.5
76	3964.0
77	3592.0
78	3220.0
79	2762.5
80	2305.0
81	1862.0
82	1419.0
83	1202.5
84	986.0
85	811.5
86	637.0
87	473.0
88	309.0
89	202.5
90	96.0
91	71.5
92	47.0
93	31.5
94	16.0
95	8.5
96	1.0
97	2.0
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013211218511804354
2	0.003849626586221137
3	6.124405932624535E-4
4	2.6247453996962295E-4
5	0.0
6	8.749151332320765E-5
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	2.6247453996962295E-4
47	8.749151332320765E-5
48	0.0
49	0.0
50	8.749151332320765E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1142968.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.77992462862451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.37667622499708	50.6762809674055
2	10.71082523991688	13.020063015850338
3	2.6528769316301632	4.8372497986049385
4	1.0529670892551304	2.5599704128539584
5	0.5501485068668416	1.6718992390958471
6	0.33142078399919295	1.2086238163098353
7	0.23109532902660695	0.9832169676185045
8	0.15902790893119442	0.7732563454948607
9	0.10963741047744317	0.5997378190766907
>10	0.6692169364155318	7.548046155302765
>50	0.07438812313540213	3.142385365705637
>100	0.07390915713699882	9.293387509874313
>500	0.006653268106101555	2.8098613127768335
>1k	0.0011570901054089662	0.876021274030008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1442	0.12616276221206543	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1358	0.118813475092916	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1302	0.11391395034681635	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1271	0.11120171343379692	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1199	0.10490232447452597	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1164	0.1018401215082137	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1144	0.10009029124174953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.6247453996962295E-4
2	0.0	0.0	0.0	0.0	2.6247453996962295E-4
3	0.0	0.0	0.0	0.0	2.6247453996962295E-4
4	0.0	0.0	0.0	0.0	2.6247453996962295E-4
5	0.0	0.0	0.0	0.0	3.499660532928306E-4
6	0.0	0.0	0.0	0.0	4.3745756661603825E-4
7	0.0	0.0	0.0	0.0	4.3745756661603825E-4
8	0.0	0.0	0.0	0.0	4.3745756661603825E-4
9	0.0	0.0	0.0	0.0	4.3745756661603825E-4
10	0.0	0.0	0.0	0.0	4.3745756661603825E-4
11	0.0	0.0	0.0	0.0	4.3745756661603825E-4
12	0.0	0.0	0.0	0.0	9.624066465552841E-4
13	0.0	0.0	0.0	0.0	0.0010498981598784918
14	0.0	0.0	0.0	0.0	0.0010498981598784918
15	0.0	0.0	0.0	0.0	0.0015748472398177376
16	0.0	0.0	0.0	0.0	0.0016623387531409454
17	0.0	0.0	0.0	0.0	0.0016623387531409454
18	0.0	0.0	0.0	0.0	0.001749830266464153
19	0.0	0.0	0.0	0.0	0.0018373217797873606
20	0.0	0.0	0.0	0.0	0.0018373217797873606
21	0.0	0.0	0.0	8.749151332320765E-5	0.0018373217797873606
22	0.0	0.0	0.0	2.6247453996962295E-4	0.0018373217797873606
23	0.0	0.0	0.0	4.3745756661603825E-4	0.002012304806433776
24	0.0	0.0	0.0	0.0010498981598784918	0.002012304806433776
25	0.0	0.0	0.0	0.0013123726998481148	0.0020997963197569836
26	0.0	0.0	0.0	0.0019248132931105682	0.002274779346403399
27	0.0	0.0	0.0	0.003324677506281891	0.0023622708597266064
28	0.0	0.0	0.0	0.009011625872290388	0.002449762373049814
29	0.0	0.0	0.0	0.019948065037691344	0.002449762373049814
30	0.0	0.0	0.0	0.042958333041694954	0.002449762373049814
31	0.0	0.0	0.0	0.08775398786317727	0.002449762373049814
32	0.0	0.0	0.0	0.14926052172939225	0.002449762373049814
33	0.0	0.0	0.0	0.2161040379083229	0.002449762373049814
34	0.0	0.0	0.0	0.28775958732002993	0.002537253886373022
35	0.0	0.0	0.0	0.37192642313695573	0.002537253886373022
36	0.0	0.0	0.0	0.48531542440383285	0.002537253886373022
37	0.0	0.0	0.0	0.6546989941975628	0.002537253886373022
38	0.0	0.0	0.0	0.8875139111506184	0.002712236913019437
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	55	0.0044835415	19.99994	4
GATTTCG	335	0.0	19.701433	41
GTCCTAC	660	0.0	19.66919	1
GTCCTAA	350	0.0	19.488214	1
TCCTACA	665	0.0	19.187912	2
TTAGAAC	185	1.8189894E-12	19.026972	3
TTAACGG	140	4.0799932E-9	18.857086	35
TACACGG	70	8.123117E-4	18.857086	5
TAGGACG	570	0.0	18.52626	4
GACTGCG	95	1.5970269E-5	18.52626	7
ATAACCG	60	0.0074119885	18.333279	5
ATAACGC	410	0.0	18.243849	3
TAACGCC	415	0.0	18.024042	4
CGTGATC	75	0.0012908381	17.599947	38
ATAGCAC	125	4.8520633E-7	17.599947	3
ACGTGAT	75	0.0012908381	17.599947	37
AATAACG	440	0.0	17.499949	2
TAGCACT	190	3.274181E-11	17.36837	4
ATTTCGT	355	0.0	17.352062	42
GATATAC	305	0.0	17.313696	1
>>END_MODULE