FastQCFastQC Report
Thu 2 Feb 2017
SRR4062638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109149
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT34380.3099673713811219No Hit
GTCCTAAAGTGTGTATTTCTCATTT24640.22215229874435263No Hit
GTCCTACAGTGGACATTTCTAAATT23640.21313637752907857No Hit
CTGTAGGACGTGGAATATGGCAAGA21640.1951045350985305No Hit
TATCAACGCAGAGTACTTTTTTTTT21080.19005561921797703No Hit
CTTTAGGACGTGAAATATGGCGAGG18700.16859772672562479No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15740.1419105999284136No Hit
GTCCTACAGTGTGCATTTCTCATTT13160.11864952319300653No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12920.11648570210134077No Hit
GGTATCAACGCAGAGTACTTTTTTT12380.11161710464509278No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11800.10638787034023382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTC953.0377123E-1015.00046357
TCCAACG1101.8553692E-1013.82235118
GCGCCAC500.001497196613.30221113
TGCCCAA550.003063671312.0929212
GGTGCTC550.0030667812.0912838
TAGGACC4350.012.0086144
GTATATA3400.012.0080321
GGCGAGG5800.011.95888519
CTTAGAC1157.1057E-811.5603573
GTCCTAA7350.011.4970111
CCACCTT4400.011.2291413
AGGACGT12750.011.1747995
ATTGGAC855.342254E-511.1717743
TATGTAC1701.2732926E-1111.1717743
GTATCAA27900.011.1622551
CCTGTAC1451.2241799E-911.133253
CCGTGTC600.00587053211.0841769
TGTGCGC600.00587053211.08417610
TAGACAG2150.011.0448595
GACGTGA7450.010.9667827