##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062638_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109149
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.135870834306303	32.0	32.0	32.0	32.0	32.0
2	30.838215604936757	32.0	32.0	32.0	32.0	32.0
3	30.822571178444015	32.0	32.0	32.0	32.0	32.0
4	30.88787710217473	32.0	32.0	32.0	32.0	32.0
5	30.751462607819146	32.0	32.0	32.0	32.0	32.0
6	34.37309775332259	36.0	36.0	36.0	32.0	36.0
7	34.246320377154014	36.0	36.0	36.0	32.0	36.0
8	34.26136794966231	36.0	36.0	36.0	32.0	36.0
9	34.3840457864543	36.0	36.0	36.0	32.0	36.0
10	34.099376188411114	36.0	36.0	36.0	32.0	36.0
11	34.39791227328339	36.0	36.0	36.0	32.0	36.0
12	34.202539063732644	36.0	36.0	36.0	32.0	36.0
13	34.27875154735748	36.0	36.0	36.0	32.0	36.0
14	34.2211073534755	36.0	36.0	36.0	32.0	36.0
15	34.16940645485864	36.0	36.0	36.0	32.0	36.0
16	34.172995693094435	36.0	36.0	36.0	32.0	36.0
17	34.132415933296606	36.0	36.0	36.0	32.0	36.0
18	34.14703254477081	36.0	36.0	36.0	32.0	36.0
19	34.109406400763106	36.0	36.0	36.0	32.0	36.0
20	34.10255339904738	36.0	36.0	36.0	32.0	36.0
21	34.07773617431022	36.0	36.0	36.0	32.0	36.0
22	34.05006000095569	36.0	36.0	36.0	32.0	36.0
23	34.00917640461291	36.0	36.0	36.0	32.0	36.0
24	33.994519221493235	36.0	36.0	36.0	32.0	36.0
25	33.54625302822254	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	44.0
5	88.0
6	169.0
7	49.0
8	183.0
9	179.0
10	143.0
11	47.0
12	90.0
13	57.0
14	227.0
15	266.0
16	412.0
17	537.0
18	726.0
19	918.0
20	1510.0
21	2031.0
22	3061.0
23	4419.0
24	6341.0
25	8939.0
26	12568.0
27	16254.0
28	22074.0
29	29507.0
30	38872.0
31	52595.0
32	75279.0
33	109211.0
34	245591.0
35	476757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.10789755575032	20.416445757277387	12.366056776418057	24.109599910554238
2	13.35297071793665	22.64114708292873	43.17231218933396	20.83357000980066
3	18.03320370789465	27.94499649713775	30.304041230086202	23.717758564881393
4	10.788406811205812	18.101417859036463	40.33864865157313	30.771526678184593
5	11.29940700322428	40.31638519988275	35.888074677008404	12.496133119884558
6	28.129653766534823	39.49812609085866	20.190953822007945	12.181266320598569
7	26.07348292387194	33.70911400444586	23.393700020208435	16.823703051473775
8	24.139558429534524	38.01815768362749	21.89109901721831	15.951184869619675
9	27.064607654155203	15.679755690801587	21.6617754290211	35.593861226022106
10	13.731086505402695	30.971871324601285	35.66997300232067	19.627069167675355
11	32.59858966767285	23.843859674460926	25.761944135530552	17.795606522335678
12	22.37725053106349	26.934691604005838	33.166799526579354	17.521258338351313
13	27.968453244943202	23.947207839569025	27.0391472952187	21.04519162026907
14	20.06057132024716	23.952662294578822	29.64293612930307	26.343830255870948
15	21.445392289688627	33.297980942202905	25.036533311429892	20.22009345667858
16	19.875531967705104	30.368665466213564	29.93379456005199	19.822008006029343
17	19.35535120604226	30.42542992985177	29.652725183404538	20.566493680701427
18	19.25371355730533	29.491522057456095	32.33363245216657	18.92113193307201
19	21.735507570197317	28.27446806397767	29.754473011170003	20.23555135465501
20	22.564376607327745	28.613480385298296	30.229270718900324	18.59287228847364
21	22.344065403517895	28.130518150992863	28.32948755448187	21.195928891007377
22	21.64433408577878	30.18221218961625	29.071963882618512	19.101489841986456
23	20.430887071582184	29.934580569905616	29.536069164390018	20.098463194122182
24	20.92042352193445	28.739491398113508	30.69522887807133	19.644856201880714
25	20.551322560259997	29.409136047666333	30.231922000541662	19.807619391532004
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	337.0
1	337.0
2	627.5
3	918.0
4	918.0
5	918.0
6	2036.5
7	3155.0
8	3155.0
9	3155.0
10	3825.5
11	4496.0
12	4496.0
13	4496.0
14	6064.5
15	7633.0
16	7633.0
17	7633.0
18	12795.5
19	17958.0
20	17958.0
21	17958.0
22	28171.0
23	38384.0
24	38384.0
25	38384.0
26	54405.0
27	70426.0
28	70426.0
29	70426.0
30	90124.5
31	109823.0
32	109823.0
33	109823.0
34	121572.0
35	133321.0
36	133321.0
37	133321.0
38	139537.0
39	145753.0
40	145753.0
41	145753.0
42	147894.0
43	150035.0
44	150035.0
45	150035.0
46	148921.0
47	147807.0
48	147807.0
49	147807.0
50	134134.5
51	120462.0
52	120462.0
53	120462.0
54	97753.5
55	75045.0
56	75045.0
57	75045.0
58	59299.5
59	43554.0
60	43554.0
61	43554.0
62	33060.0
63	22566.0
64	22566.0
65	22566.0
66	16545.5
67	10525.0
68	10525.0
69	10525.0
70	7294.0
71	4063.0
72	4063.0
73	4063.0
74	2791.0
75	1519.0
76	1519.0
77	1519.0
78	1028.0
79	537.0
80	537.0
81	537.0
82	375.0
83	213.0
84	213.0
85	213.0
86	159.5
87	106.0
88	106.0
89	106.0
90	81.0
91	56.0
92	56.0
93	56.0
94	56.0
95	56.0
96	56.0
97	56.0
98	228.5
99	401.0
100	401.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00874544357881583
2	0.003606368486109621
3	0.005048915880553469
4	0.0137943594593693
5	0.03371954534512496
6	0.04544024292498122
7	0.0632016077190711
8	0.08727411736385282
9	0.09421637669961384
10	0.11504315470689691
11	0.1194609561023812
12	0.1321734050159176
13	0.12018222979960312
14	0.12297716537633809
15	0.1132399704638421
16	0.11125646779648181
17	0.12189525483050519
18	0.1308210168336265
19	0.13073085762147377
20	0.12009207058745039
21	0.1299194247120991
22	0.14867254083986914
23	0.13767311695723478
24	0.13388643004681966
25	0.13064069840932102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1109149.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.1007585617898
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.15883105420369	67.85054675707363
2	8.288278956257296	12.614886314857992
3	1.2780681018457842	2.9178585613227304
4	0.39029415538782775	1.18806725148987
5	0.19525330525513376	0.7429462320806188
6	0.12358647259628261	0.5643014587531773
7	0.09043400473900745	0.4817467452293273
8	0.060474264471467254	0.3681709919795962
9	0.04849737535370377	0.3321618347405435
>10	0.3107644382048724	4.505812992878172
>50	0.02700097368957421	1.4426161806342168
>100	0.025071111115451017	3.9797953869461056
>500	0.002019944033029204	1.0153610108115911
>1k	0.001425842846844144	1.9957282812024781
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3438	0.3099673713811219	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2464	0.22215229874435263	No Hit
GTCCTACAGTGGACATTTCTAAATT	2364	0.21313637752907857	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2164	0.1951045350985305	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2108	0.19005561921797703	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1870	0.16859772672562479	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1574	0.1419105999284136	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1316	0.11864952319300653	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1292	0.11648570210134077	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1238	0.11161710464509278	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1180	0.10638787034023382	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.8031842430548105E-4	0.0
11	0.0	0.0	0.0	2.7047763645822155E-4	0.0
12	0.0	0.0	0.0	3.606368486109621E-4	0.0
13	0.0	0.0	0.0	3.606368486109621E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGTC	95	3.0377123E-10	15.0004635	7
TCCAACG	110	1.8553692E-10	13.822351	18
GCGCCAC	50	0.0014971966	13.302211	13
TGCCCAA	55	0.0030636713	12.09292	12
GGTGCTC	55	0.00306678	12.091283	8
TAGGACC	435	0.0	12.008614	4
GTATATA	340	0.0	12.008032	1
GGCGAGG	580	0.0	11.958885	19
CTTAGAC	115	7.1057E-8	11.560357	3
GTCCTAA	735	0.0	11.497011	1
CCACCTT	440	0.0	11.22914	13
AGGACGT	1275	0.0	11.174799	5
ATTGGAC	85	5.342254E-5	11.171774	3
TATGTAC	170	1.2732926E-11	11.171774	3
GTATCAA	2790	0.0	11.162255	1
CCTGTAC	145	1.2241799E-9	11.13325	3
CCGTGTC	60	0.005870532	11.084176	9
TGTGCGC	60	0.005870532	11.084176	10
TAGACAG	215	0.0	11.044859	5
GACGTGA	745	0.0	10.966782	7
>>END_MODULE