FastQCFastQC Report
Thu 2 Feb 2017
SRR4062638_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062638_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109149
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA12370.11152694543294003No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAACG508.339135E-526.4007844
TAGGACC5900.023.8651144
GTATCAA32750.022.0411991
TAGGACG18450.020.629344
AGGACGT18300.020.3175455
TAAGACG654.930821E-420.3082964
GTAGGAC15950.020.1385293
GGACGTG18300.020.07716
TATTGCG550.004483781819.99969316
CTAGGAC2450.019.755693
GACGTGA10250.019.3167767
GACGTGG9550.019.1201257
TGTAGGA18100.019.085162
TTAGGAC12100.018.9096533
GTCCTAA11950.018.7847291
CTGTAGG18550.018.7450181
GCACTCG951.5971273E-518.52603113
TACTCGC600.007412384718.3330544
GTCCTAC15650.017.7185781
AGGCGGA750.001290912717.5997332
Produced by FastQC (version 0.11.5)