##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062638_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109149
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285361119200395	32.0	32.0	32.0	32.0	32.0
2	31.446801106073213	32.0	32.0	32.0	32.0	32.0
3	31.526145720728234	32.0	32.0	32.0	32.0	32.0
4	31.61131462048832	32.0	32.0	32.0	32.0	32.0
5	31.57290589451913	32.0	32.0	32.0	32.0	32.0
6	35.15477451631837	36.0	36.0	36.0	36.0	36.0
7	35.16158784798075	36.0	36.0	36.0	36.0	36.0
8	35.11751532030412	36.0	36.0	36.0	36.0	36.0
9	35.24173307643969	36.0	36.0	36.0	36.0	36.0
10	35.14045993820488	36.0	36.0	36.0	36.0	36.0
11	35.24666658852868	36.0	36.0	36.0	36.0	36.0
12	35.179117503599606	36.0	36.0	36.0	36.0	36.0
13	35.203187308468024	36.0	36.0	36.0	36.0	36.0
14	35.17791027174888	36.0	36.0	36.0	36.0	36.0
15	35.151336745558986	36.0	36.0	36.0	36.0	36.0
16	35.16497062162072	36.0	36.0	36.0	36.0	36.0
17	35.160893622047176	36.0	36.0	36.0	36.0	36.0
18	35.159478122416374	36.0	36.0	36.0	36.0	36.0
19	35.14904579997818	36.0	36.0	36.0	36.0	36.0
20	35.129622800904116	36.0	36.0	36.0	36.0	36.0
21	35.11879918748518	36.0	36.0	36.0	36.0	36.0
22	35.099875670446444	36.0	36.0	36.0	36.0	36.0
23	35.07096431588542	36.0	36.0	36.0	36.0	36.0
24	35.06531223487557	36.0	36.0	36.0	36.0	36.0
25	35.04114505805803	36.0	36.0	36.0	36.0	36.0
26	35.004211336799656	36.0	36.0	36.0	36.0	36.0
27	34.98213585370406	36.0	36.0	36.0	36.0	36.0
28	34.960915981531784	36.0	36.0	36.0	36.0	36.0
29	34.94563129029553	36.0	36.0	36.0	36.0	36.0
30	34.920099103005995	36.0	36.0	36.0	36.0	36.0
31	34.907392965237314	36.0	36.0	36.0	36.0	36.0
32	34.879292141993545	36.0	36.0	36.0	36.0	36.0
33	34.86965412221442	36.0	36.0	36.0	36.0	36.0
34	34.848277373013005	36.0	36.0	36.0	36.0	36.0
35	34.82914829297056	36.0	36.0	36.0	36.0	36.0
36	34.78548148174862	36.0	36.0	36.0	32.0	36.0
37	34.77141303828431	36.0	36.0	36.0	32.0	36.0
38	34.73891515026385	36.0	36.0	36.0	32.0	36.0
39	34.69815597363384	36.0	36.0	36.0	32.0	36.0
40	34.691186666534435	36.0	36.0	36.0	32.0	36.0
41	34.65114155086467	36.0	36.0	36.0	32.0	36.0
42	34.64810408700725	36.0	36.0	36.0	32.0	36.0
43	34.61777723281543	36.0	36.0	36.0	32.0	36.0
44	34.58977558470503	36.0	36.0	36.0	32.0	36.0
45	34.55372993168636	36.0	36.0	36.0	32.0	36.0
46	34.49969120469838	36.0	36.0	36.0	32.0	36.0
47	34.4803304154807	36.0	36.0	36.0	32.0	36.0
48	34.43215925001961	36.0	36.0	36.0	32.0	36.0
49	34.39012071416915	36.0	36.0	36.0	32.0	36.0
50	33.879209195518364	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	5.0
21	31.0
22	87.0
23	281.0
24	685.0
25	1596.0
26	3374.0
27	6289.0
28	10730.0
29	16772.0
30	24566.0
31	35142.0
32	50732.0
33	80123.0
34	170191.0
35	708541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.45469202697621	21.115947400171507	12.898283733567062	24.531076839285216
2	12.936266284614808	23.14626557232492	42.655975706406736	21.261492436653537
3	17.432558290499962	28.30996544178704	30.73886775645701	23.518608511255987
4	10.697158622181952	18.574126917580656	40.39958706931916	30.32912739091823
5	11.026922442340929	40.642330291060986	35.777429362511256	12.553317904086828
6	28.301129791199152	39.847341596169294	19.825216405819628	12.026312206811927
7	26.061445361664994	33.67043893150418	23.385337213789324	16.882778493041506
8	24.14887449747509	38.370768940872686	21.645243335205638	15.835113226446582
9	26.959678095548927	15.544439926466147	21.467359209628285	36.02852276835664
10	13.663267964899214	31.13621343931248	35.52155751842178	19.678961077366523
11	32.7701688411566	23.760107974672472	25.777510505802198	17.692212678368733
12	22.45622544851954	26.908197185409716	33.174082111600875	17.46149525446987
13	28.146263486691147	23.89318297180992	26.944351029482966	21.01620251201597
14	20.108209086425717	23.758935904914487	29.622981222540883	26.50987378611891
15	21.412452249427265	33.33140993680741	24.960938521334825	20.295199292430503
16	19.904359107748373	30.332444062970797	29.804922512665115	19.95827431661571
17	19.414253630486076	30.5659564224464	29.42679477689652	20.592995170171005
18	19.312824516814242	29.45771938666491	32.35146945991927	18.877986636601573
19	21.919417499362122	28.126428460017543	29.771743922592908	20.182410118027423
20	22.67819742883959	28.667113255297526	30.10416093780006	18.550528378062822
21	22.404113423895254	28.024999346345712	28.357506520765018	21.213380708994013
22	21.757130917487192	30.017247457284817	29.09365648799215	19.131965137235845
23	20.55422670894533	29.832601390796004	29.48765224509962	20.125519655159046
24	21.03829151899339	28.765296637331865	30.500681152847815	19.69573069082693
25	20.71984918166991	29.267753926659086	30.14761767805768	19.86477921361332
26	19.31976677615	29.949988685018873	30.656566430659904	20.07367810817122
27	20.17799231663194	29.885885485178278	29.823675628792884	20.112446569396898
28	19.476012690810705	30.195672538135092	29.380182464213554	20.948132306840648
29	20.400324933800597	29.328611394862186	29.21816635997508	21.052897311362134
30	19.947274892733077	29.45898161563505	29.952422983747002	20.641320507884874
31	20.984827106186817	28.69100544651801	29.70700960826724	20.61715783902794
32	19.857025521368186	29.863886637413007	30.001379435945935	20.27770840527287
33	19.64668407941584	29.781481117505404	30.634477423682483	19.937357379396275
34	20.066826008047613	29.83458489346337	30.70615399734391	19.39243510114511
35	19.981174756502508	29.96351256684179	30.13598713968998	19.91932553696573
36	19.40983582909059	29.643267045275252	30.836343899692466	20.11055322594169
37	19.694558621068943	29.1513583837699	29.46583371575866	21.6882492794025
38	19.79571725710432	30.06259754099765	29.582499736284305	20.559185465613727
39	20.813795080733065	28.55486503616737	30.067015342393134	20.564324540706433
40	21.45311405410815	28.288264245831712	30.414759423666254	19.843862276393885
41	20.952009152963218	27.757947759949293	30.948411800398322	20.341631286689164
42	20.013451754453186	29.26054118968687	30.668016650603303	20.057990405256643
43	19.168479618157704	29.21329776251883	30.947780685913255	20.670441933410206
44	18.906206470005383	29.068231590165073	30.729324914867163	21.29623702496238
45	19.137555008389313	29.1876023870553	30.44694626240478	21.227896342150604
46	19.234673821708196	28.163599447503852	30.696083840930154	21.905642889857802
47	20.218744280524977	28.259232979518533	30.455601546771444	21.066421193185043
48	18.69586502805304	28.727790405076327	30.656927067508516	21.919417499362122
49	19.734859788901222	28.287633131346645	30.46768288119991	21.509824198552224
50	18.795490957481817	29.729549411305424	29.889311535240083	21.58564809597268
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	54.0
1	46.0
2	38.0
3	128.5
4	219.0
5	245.0
6	271.0
7	282.5
8	294.0
9	443.0
10	592.0
11	863.0
12	1134.0
13	1844.5
14	2555.0
15	3496.0
16	4437.0
17	4926.0
18	5415.0
19	6030.0
20	6645.0
21	7833.5
22	9022.0
23	10838.5
24	12655.0
25	15461.0
26	18267.0
27	23305.5
28	28344.0
29	34616.5
30	40889.0
31	48305.0
32	55721.0
33	65583.5
34	75446.0
35	84877.0
36	94308.0
37	96985.5
38	99663.0
39	97040.0
40	94417.0
41	91603.0
42	88789.0
43	86100.0
44	83411.0
45	80786.5
46	78162.0
47	74530.0
48	70898.0
49	66359.0
50	61820.0
51	56040.5
52	50261.0
53	44552.0
54	38843.0
55	34022.0
56	29201.0
57	25013.0
58	20825.0
59	17376.5
60	13928.0
61	11536.0
62	9144.0
63	7290.5
64	5437.0
65	4386.5
66	3336.0
67	2632.0
68	1928.0
69	1523.5
70	1119.0
71	880.0
72	641.0
73	521.5
74	402.0
75	344.0
76	286.0
77	216.0
78	146.0
79	115.5
80	85.0
81	65.5
82	46.0
83	37.0
84	28.0
85	19.5
86	11.0
87	8.0
88	5.0
89	6.0
90	7.0
91	4.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01550738449027137
2	0.004507960607637027
3	5.409552729164432E-4
4	3.606368486109621E-4
5	0.0
6	5.409552729164432E-4
7	9.015921215274053E-5
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	9.015921215274053E-5
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1109149.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.50891993657024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.15400566925523	76.18817647064189
2	7.963709696348925	13.460090102536787
3	1.1018428835320313	2.7934665608126434
4	0.2861944131974342	0.967439230047827
5	0.11801399047125588	0.4986617436065263
6	0.07850397915999478	0.3980571893720506
7	0.05718896520441361	0.3383084377200564
8	0.035960608539717734	0.24311937503626804
9	0.031028828235880563	0.23599914850004589
>10	0.15227808230029555	2.3538032771728474
>50	0.012903147858507637	0.7389022594921499
>100	0.007618609493037149	1.2245635202710359
>500	5.365188594701832E-4	0.35601462157317054
>1k	2.1460754378807331E-4	0.20339806321666368
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1237	0.11152694543294003	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	9.015921215274053E-5	0.0
11	0.0	0.0	0.0	1.8031842430548105E-4	0.0
12	0.0	0.0	0.0	2.7047763645822155E-4	0.0
13	9.015921215274053E-5	0.0	0.0	2.7047763645822155E-4	0.0
14	9.015921215274053E-5	0.0	0.0	3.606368486109621E-4	0.0
15	9.015921215274053E-5	0.0	0.0	3.606368486109621E-4	0.0
16	9.015921215274053E-5	0.0	0.0	4.507960607637026E-4	0.0
17	9.015921215274053E-5	0.0	0.0	4.507960607637026E-4	0.0
18	9.015921215274053E-5	0.0	0.0	5.409552729164431E-4	0.0
19	9.015921215274053E-5	0.0	0.0	7.212736972219242E-4	0.0
20	9.015921215274053E-5	0.0	0.0	0.002253980303818513	0.0
21	9.015921215274053E-5	0.0	0.0	0.002704776364582216	0.0
22	9.015921215274053E-5	0.0	0.0	0.004147323759026064	0.0
23	9.015921215274053E-5	0.0	0.0	0.005499711941317172	0.0
24	9.015921215274053E-5	0.0	0.0	0.008294647518052128	0.0
25	9.015921215274053E-5	0.0	0.0	0.010187990973259679	0.0
26	9.015921215274053E-5	0.0	0.0	0.012622289701383673	0.0
27	9.015921215274053E-5	0.0	0.0	0.015777862126729592	0.0
28	9.015921215274053E-5	0.0	0.0	0.02587569388783653	0.0
29	9.015921215274053E-5	0.0	0.0	0.04246498892394079	0.0
30	9.015921215274053E-5	0.0	0.0	0.08312679360482676	0.0
31	9.015921215274053E-5	0.0	0.0	0.1849165441252708	0.0
32	9.015921215274053E-5	0.0	0.0	0.31672931229257745	0.0
33	9.015921215274053E-5	0.0	0.0	0.46053325567619857	0.0
34	9.015921215274053E-5	0.0	0.0	0.6416631128910543	0.0
35	9.015921215274053E-5	0.0	0.0	0.8390216282934033	0.0
36	9.015921215274053E-5	0.0	0.0	1.0921886960182987	0.0
37	9.015921215274053E-5	0.0	0.0	1.4762669397889734	0.0
38	9.015921215274053E-5	0.0	0.0	1.9734048355991847	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAACG	50	8.339135E-5	26.400784	4
TAGGACC	590	0.0	23.865114	4
GTATCAA	3275	0.0	22.041199	1
TAGGACG	1845	0.0	20.62934	4
AGGACGT	1830	0.0	20.317545	5
TAAGACG	65	4.930821E-4	20.308296	4
GTAGGAC	1595	0.0	20.138529	3
GGACGTG	1830	0.0	20.0771	6
TATTGCG	55	0.0044837818	19.999693	16
CTAGGAC	245	0.0	19.75569	3
GACGTGA	1025	0.0	19.316776	7
GACGTGG	955	0.0	19.120125	7
TGTAGGA	1810	0.0	19.08516	2
TTAGGAC	1210	0.0	18.909653	3
GTCCTAA	1195	0.0	18.784729	1
CTGTAGG	1855	0.0	18.745018	1
GCACTCG	95	1.5971273E-5	18.526031	13
TACTCGC	60	0.0074123847	18.33305	44
GTCCTAC	1565	0.0	17.718578	1
AGGCGGA	75	0.0012909127	17.59973	32
>>END_MODULE