##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062637_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4977
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.572433192686358	32.0	32.0	32.0	14.0	32.0
2	28.32670283303195	32.0	32.0	32.0	14.0	32.0
3	28.66083986337151	32.0	32.0	32.0	14.0	32.0
4	28.13522202129797	32.0	32.0	32.0	14.0	32.0
5	28.360859955796666	32.0	32.0	32.0	14.0	32.0
6	31.49708659835242	36.0	32.0	36.0	14.0	36.0
7	31.744625276270845	36.0	32.0	36.0	14.0	36.0
8	31.500904159132006	36.0	32.0	36.0	14.0	36.0
9	31.833031946955998	36.0	32.0	36.0	14.0	36.0
10	30.64155113522202	36.0	32.0	36.0	14.0	36.0
11	32.14225437010247	36.0	32.0	36.0	14.0	36.0
12	31.42977697408077	36.0	32.0	36.0	14.0	36.0
13	31.617842073538277	36.0	32.0	36.0	14.0	36.0
14	31.299377134820173	36.0	32.0	36.0	14.0	36.0
15	31.137231263813543	36.0	32.0	36.0	14.0	36.0
16	31.15591721920836	36.0	32.0	36.0	14.0	36.0
17	30.932489451476794	36.0	32.0	36.0	14.0	36.0
18	31.075547518585495	36.0	32.0	36.0	14.0	36.0
19	31.271649588105284	36.0	32.0	36.0	14.0	36.0
20	31.079766927868192	36.0	32.0	36.0	14.0	36.0
21	31.05244122965642	36.0	32.0	36.0	14.0	36.0
22	30.896925858951175	36.0	32.0	36.0	14.0	36.0
23	30.86136226642556	36.0	32.0	36.0	14.0	36.0
24	30.717902350813745	36.0	32.0	36.0	14.0	36.0
25	30.258991360257184	36.0	27.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	4.0
15	5.0
16	17.0
17	28.0
18	46.0
19	94.0
20	81.0
21	95.0
22	110.0
23	121.0
24	139.0
25	150.0
26	198.0
27	169.0
28	207.0
29	225.0
30	279.0
31	350.0
32	432.0
33	489.0
34	797.0
35	940.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.31464737793852	19.36909785011051	10.990556560176813	27.325698211774164
2	16.676712879244523	19.971870604781998	37.43218806509946	25.919228450874023
3	18.76632509543902	23.146473779385172	27.92847096644565	30.158730158730158
4	12.939521800281295	14.888487040385776	35.24211372312639	36.929877436206546
5	16.278135048231512	34.40514469453376	32.65675241157556	16.659967845659164
6	35.16881028938907	35.02813504823151	15.755627009646304	14.047427652733118
7	30.54662379421222	30.586816720257236	20.357717041800644	18.508842443729904
8	27.612540192926044	31.79260450160772	19.131832797427652	21.463022508038584
9	27.13022508038585	13.424437299035368	19.69453376205788	39.7508038585209
10	15.115577889447234	25.708542713567837	31.075376884422113	28.100502512562812
11	36.274115755627015	21.784565916398712	22.14630225080386	19.79501607717042
12	24.36670687575392	23.964616003216726	27.864897466827504	23.80377965420185
13	29.849246231155778	18.733668341708544	25.386934673366834	26.030150753768844
14	22.733668341708544	20.100502512562816	24.884422110552766	32.28140703517588
15	24.241206030150753	26.974874371859297	21.969849246231156	26.814070351758794
16	26.95477386934673	24.743718592964825	22.894472361809047	25.407035175879393
17	23.2964824120603	27.195979899497484	25.08542713567839	24.42211055276382
18	23.522316043425814	26.09569762766385	26.517893043827907	23.86409328508243
19	25.28643216080402	24.964824120603016	25.447236180904518	24.30150753768844
20	24.748693204664253	24.105347808604744	25.59308403699236	25.55287494973864
21	24.939710610932476	24.517684887459808	25.5024115755627	25.04019292604502
22	23.77889447236181	25.266331658291456	25.68844221105528	25.266331658291456
23	24.442211055276385	24.623115577889447	25.68844221105528	25.246231155778894
24	25.025125628140703	24.78391959798995	26.05025125628141	24.140703517587937
25	23.035175879396984	25.125628140703515	25.567839195979897	26.271356783919597
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	1.0
3	2.0
4	2.0
5	2.0
6	5.0
7	8.0
8	8.0
9	8.0
10	9.0
11	10.0
12	10.0
13	10.0
14	13.5
15	17.0
16	17.0
17	17.0
18	28.0
19	39.0
20	39.0
21	39.0
22	56.0
23	73.0
24	73.0
25	73.0
26	110.0
27	147.0
28	147.0
29	147.0
30	187.5
31	228.0
32	228.0
33	228.0
34	275.5
35	323.0
36	323.0
37	323.0
38	361.5
39	400.0
40	400.0
41	400.0
42	447.0
43	494.0
44	494.0
45	494.0
46	563.5
47	633.0
48	633.0
49	633.0
50	632.0
51	631.0
52	631.0
53	631.0
54	605.5
55	580.0
56	580.0
57	580.0
58	532.5
59	485.0
60	485.0
61	485.0
62	427.0
63	369.0
64	369.0
65	369.0
66	302.5
67	236.0
68	236.0
69	236.0
70	190.0
71	144.0
72	144.0
73	144.0
74	107.5
75	71.0
76	71.0
77	71.0
78	63.0
79	55.0
80	55.0
81	55.0
82	35.5
83	16.0
84	16.0
85	16.0
86	12.0
87	8.0
88	8.0
89	8.0
90	6.0
91	4.0
92	4.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	2.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.020092425155716295
6	0.020092425155716295
7	0.020092425155716295
8	0.020092425155716295
9	0.020092425155716295
10	0.04018485031143259
11	0.020092425155716295
12	0.06027727546714888
13	0.04018485031143259
14	0.04018485031143259
15	0.04018485031143259
16	0.04018485031143259
17	0.04018485031143259
18	0.06027727546714888
19	0.04018485031143259
20	0.06027727546714888
21	0.020092425155716295
22	0.04018485031143259
23	0.04018485031143259
24	0.04018485031143259
25	0.04018485031143259
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4977.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	91.58127385975487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.40544098288723	86.45770544504721
2	3.751645458534445	6.871609403254973
3	0.9433962264150944	2.591922845087402
4	0.4607283896445809	1.6877637130801686
5	0.26327336551118913	1.2055455093429777
6	0.10969723562966215	0.6027727546714888
7	0.02193944712593243	0.14064697609001406
8	0.02193944712593243	0.16073940124573036
9	0.0	0.0
>10	0.02193944712593243	0.2812939521800281
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	14	0.2812939521800281	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	8	0.16073940124573036	No Hit
GCCCGAGGTTATCTAGAGTCACCAA	7	0.14064697609001406	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6	0.12055455093429776	No Hit
ATCTGATCGTCTTCGAACCTCCGAC	6	0.12055455093429776	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	6	0.12055455093429776	No Hit
TATCTAGAGTCACCAAAGCCGCCGG	6	0.12055455093429776	No Hit
GAACTACGACGGTATCTGATCGTCT	6	0.12055455093429776	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	5	0.10046212577858146	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	5	0.10046212577858146	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5	0.10046212577858146	No Hit
GTCCTATTCCATTATTCCTAGCTGC	5	0.10046212577858146	No Hit
CTTCGGGCCCCGCGGGACACTCAGC	5	0.10046212577858146	No Hit
GAATAATTGCAATCCCCGATCCCCA	5	0.10046212577858146	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5	0.10046212577858146	No Hit
GCTTTGAACACTCTAATTTTTTCAA	5	0.10046212577858146	No Hit
GGATTGACAGATTGATAGCTCTTTC	5	0.10046212577858146	No Hit
CCATAAACGATGCCGACTGGCGATG	5	0.10046212577858146	No Hit
CTCGGGGGTCGCGTAACTAGTTAGC	5	0.10046212577858146	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5	0.10046212577858146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE