##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062637_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4977
Sequences flagged as poor quality	0
Sequence length	50
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.296363271046815	32.0	32.0	32.0	21.0	32.0
2	30.068917018284107	32.0	32.0	32.0	21.0	32.0
3	30.78902953586498	32.0	32.0	32.0	32.0	32.0
4	31.189069720715292	32.0	32.0	32.0	32.0	32.0
5	30.891299979907576	32.0	32.0	32.0	32.0	32.0
6	34.53064094836247	36.0	36.0	36.0	32.0	36.0
7	34.54108900944344	36.0	36.0	36.0	32.0	36.0
8	34.5778581474784	36.0	36.0	36.0	32.0	36.0
9	34.826200522403056	36.0	36.0	36.0	32.0	36.0
10	34.452883263009845	36.0	36.0	36.0	32.0	36.0
11	34.90496282901346	36.0	36.0	36.0	32.0	36.0
12	34.66927868193691	36.0	36.0	36.0	32.0	36.0
13	34.73839662447257	36.0	36.0	36.0	32.0	36.0
14	34.687964637331724	36.0	36.0	36.0	32.0	36.0
15	34.62949568012859	36.0	36.0	36.0	32.0	36.0
16	34.63592525617842	36.0	36.0	36.0	32.0	36.0
17	34.534458509142056	36.0	36.0	36.0	32.0	36.0
18	34.66365280289331	36.0	36.0	36.0	32.0	36.0
19	34.74422342776773	36.0	36.0	36.0	32.0	36.0
20	34.74040586698815	36.0	36.0	36.0	32.0	36.0
21	34.62286517982721	36.0	36.0	36.0	32.0	36.0
22	34.769539883463935	36.0	36.0	36.0	32.0	36.0
23	34.62386980108499	36.0	36.0	36.0	32.0	36.0
24	34.499095840867994	36.0	36.0	36.0	32.0	36.0
25	34.597146875627885	36.0	36.0	36.0	32.0	36.0
26	34.36146272855134	36.0	36.0	36.0	32.0	36.0
27	34.58870805706249	36.0	36.0	36.0	32.0	36.0
28	34.385573638738194	36.0	36.0	36.0	32.0	36.0
29	34.424552943540284	36.0	36.0	36.0	32.0	36.0
30	34.33795459111915	36.0	36.0	36.0	32.0	36.0
31	34.354631303998396	36.0	36.0	36.0	32.0	36.0
32	34.347799879445446	36.0	36.0	36.0	32.0	36.0
33	34.278681936909784	36.0	36.0	36.0	32.0	36.0
34	34.216194494675506	36.0	36.0	36.0	32.0	36.0
35	34.31464737793852	36.0	36.0	36.0	32.0	36.0
36	34.205143660839866	36.0	36.0	36.0	32.0	36.0
37	34.11372312638135	36.0	36.0	36.0	32.0	36.0
38	33.99055656017681	36.0	36.0	36.0	32.0	36.0
39	34.299778983323286	36.0	36.0	36.0	32.0	36.0
40	34.132208157524616	36.0	36.0	36.0	32.0	36.0
41	34.12638135422946	36.0	36.0	36.0	32.0	36.0
42	34.00060277275467	36.0	36.0	36.0	32.0	36.0
43	34.048623668876836	36.0	36.0	36.0	32.0	36.0
44	34.03676913803496	36.0	36.0	36.0	32.0	36.0
45	33.97830018083182	36.0	36.0	36.0	32.0	36.0
46	34.03777375929275	36.0	36.0	36.0	32.0	36.0
47	34.0126582278481	36.0	36.0	36.0	32.0	36.0
48	33.85453084187262	36.0	36.0	36.0	32.0	36.0
49	33.75708257986739	36.0	36.0	36.0	32.0	36.0
50	33.267430178822586	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	0.0
21	1.0
22	0.0
23	4.0
24	4.0
25	12.0
26	21.0
27	40.0
28	66.0
29	112.0
30	172.0
31	270.0
32	394.0
33	620.0
34	1153.0
35	2107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.06268836648584	17.681334137030337	12.899336949969861	25.356640546513965
2	19.328912999799076	19.59011452682339	35.04118947156922	26.03978300180832
3	21.217600964436407	22.845087402049426	28.83263009845288	27.10468153506128
4	16.435603777375928	15.02913401647579	35.94534860357645	32.589913602571826
5	19.288728149487643	32.4291742013261	31.685754470564596	16.59634317862166
6	36.427566807313646	34.05666063893912	15.250150693188669	14.26562186055857
7	31.163351416515972	29.576049829214384	19.389190275266223	19.871408479003417
8	28.470966445649992	31.384368093228854	19.107896323086194	21.036769138034963
9	26.763110307414106	13.160538476994175	17.962628089210366	42.11372312638135
10	15.873015873015872	26.100060277275468	30.078360458107294	27.94856339160137
11	39.56198513160539	19.95177817962628	21.036769138034963	19.449467550733374
12	24.934699618243922	23.246935905163753	27.88828611613422	23.930078360458108
13	29.01346192485433	19.20835844886478	24.392204139039585	27.38597548724131
14	23.508137432188065	18.32429174201326	25.035161744022506	33.132409081776174
15	26.582278481012654	25.356640546513965	21.80028129395218	26.260799678521195
16	26.441631504922647	25.939320876029736	22.845087402049426	24.77396021699819
17	25.01506931886679	26.883664858348403	24.311834438416717	23.789431384368093
18	25.899136025718306	24.25155716294957	25.195901145268234	24.653405666063893
19	25.17580872011252	24.653405666063893	25.658026923849707	24.51275868997388
20	26.240707253365482	24.271649588105284	24.271649588105284	25.21599357042395
21	26.803295157725536	24.151095037170986	24.35201928872815	24.693590516375327
22	27.245328511151296	24.13100261201527	22.98573437813944	25.637934498693994
23	24.733775366686757	24.23146473779385	26.441631504922647	24.593128390596743
24	23.58850713281093	25.437010247136833	24.452481414506728	26.522001205545507
25	25.035161744022506	24.110910186859556	25.25617842073538	25.59774964838256
26	24.733775366686757	25.33654812135825	24.794052642153908	25.135623869801083
27	25.81876632509544	24.552943540285312	24.854329917621058	24.77396021699819
28	25.698211774161138	25.637934498693994	24.552943540285312	24.110910186859556
29	25.658026923849707	25.21599357042395	25.17580872011252	23.95017078561382
30	25.718304199316854	23.809523809523807	25.617842073538277	24.854329917621058
31	25.738396624472575	24.814145067309624	24.814145067309624	24.633313240908176
32	25.637934498693994	24.67349809121961	24.151095037170986	25.53747237291541
33	24.633313240908176	24.392204139039585	25.577657223226847	25.396825396825395
34	26.180429977898335	24.25155716294957	24.83423749246534	24.733775366686757
35	26.522001205545507	23.97026321076954	24.67349809121961	24.83423749246534
36	24.552943540285312	25.05525416917822	26.180429977898335	24.211372312638137
37	27.848101265822784	24.392204139039585	24.01044806108097	23.749246534056663
38	26.140245127586898	24.5328511151296	24.090817761703835	25.236085995579664
39	24.794052642153908	24.693590516375327	24.57303596544103	25.939320876029736
40	25.53747237291541	24.271649588105284	25.75848904962829	24.432388989351015
41	25.85895117540687	23.32730560578662	24.934699618243922	25.87904360056259
42	25.075346594333936	25.49728752260398	24.050632911392405	25.37673297166968
43	26.300984528832632	23.930078360458108	25.01506931886679	24.753867791842477
44	25.49728752260398	24.13100261201527	25.115531444645367	25.25617842073538
45	25.437010247136833	24.633313240908176	25.155716294956804	24.77396021699819
46	24.372111713883864	24.653405666063893	25.617842073538277	25.356640546513965
47	25.37673297166968	24.211372312638137	24.974884468555356	25.437010247136833
48	25.85895117540687	24.492666264818162	24.171187462326703	25.477195097448263
49	25.698211774161138	24.633313240908176	24.87442234277677	24.794052642153908
50	25.135623869801083	24.67349809121961	25.617842073538277	24.57303596544103
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	0.5
6	0.0
7	1.0
8	2.0
9	2.5
10	3.0
11	2.5
12	2.0
13	3.0
14	4.0
15	5.5
16	7.0
17	7.0
18	7.0
19	10.0
20	13.0
21	16.0
22	19.0
23	21.5
24	24.0
25	28.5
26	33.0
27	43.0
28	53.0
29	61.0
30	69.0
31	74.5
32	80.0
33	90.5
34	101.0
35	118.0
36	135.0
37	155.0
38	175.0
39	178.5
40	182.0
41	204.5
42	227.0
43	254.0
44	281.0
45	310.0
46	339.0
47	350.5
48	362.0
49	370.5
50	379.0
51	347.5
52	316.0
53	319.5
54	323.0
55	336.0
56	349.0
57	334.5
58	320.0
59	288.5
60	257.0
61	246.5
62	236.0
63	204.0
64	172.0
65	145.5
66	119.0
67	100.5
68	82.0
69	81.0
70	80.0
71	69.0
72	58.0
73	57.0
74	56.0
75	42.5
76	29.0
77	24.0
78	19.0
79	16.5
80	14.0
81	11.5
82	9.0
83	6.5
84	4.0
85	7.5
86	11.0
87	6.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	11.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4977.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.00783604581073
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.48498595809029	88.80851918826602
2	3.1540289479369195	5.866988145469159
3	0.8425145819831497	2.3508137432188065
4	0.19442644199611148	0.7233273056057866
5	0.1512205659969756	0.7032348804500703
6	0.02160293799956794	0.12055455093429776
7	0.04320587599913588	0.2812939521800281
8	0.04320587599913588	0.3214788024914607
9	0.02160293799956794	0.18083182640144665
>10	0.04320587599913588	0.6429576049829214
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	22	0.44203335342575845	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	10	0.20092425155716293	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	9	0.18083182640144665	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	8	0.16073940124573036	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	8	0.16073940124573036	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	7	0.14064697609001406	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	7	0.14064697609001406	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	6	0.12055455093429776	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	5	0.10046212577858146	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	5	0.10046212577858146	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	5	0.10046212577858146	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	5	0.10046212577858146	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	5	0.10046212577858146	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	5	0.10046212577858146	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG	5	0.10046212577858146	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.020092425155716295	0.0
31	0.0	0.0	0.0	0.04018485031143259	0.0
32	0.0	0.0	0.0	0.04018485031143259	0.0
33	0.0	0.0	0.0	0.12055455093429777	0.0
34	0.0	0.0	0.0	0.16073940124573036	0.0
35	0.0	0.0	0.0	0.2812939521800281	0.0
36	0.0	0.0	0.0	0.3817560779586096	0.0
37	0.0	0.0	0.0	0.5224030540486236	0.0
38	0.0	0.0	0.0	0.683142455294354	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE