FastQCFastQC Report
Thu 2 Feb 2017
SRR4062636_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062636_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237931
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54850.24509245369942148No Hit
TATCAACGCAGAGTACTTTTTTTTT37300.16667180534163029No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30730.1373143318538418No Hit
GCGCAAGACGGACCAGAGCGAAAGC28700.1282434534398067No Hit
GTCTTGCGCCGGTCCAAGAATTTCA28040.1252943008519923No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG26230.117206473300562No Hit
GAATAGGACCGCGGTTCTATTTTGT25520.11403390006215562No Hit
GTATCTGATCGTCTTCGAACCTCCG25080.11206779833694605No Hit
GATTAAGAGGGACGGCCGGGGGCAT24440.10920801400936847No Hit
GAACTACGACGGTATCTGATCGTCT23970.1071078598938037No Hit
GTACATGGAAGCAGTGGTATCAACG23620.1055439153396597No Hit
CTATTGGAGCTGGAATTACCGCGGC23540.10518644229871252No Hit
GAATAACGCCGCCGCATCGCCAGTC23140.10339907709397654No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22980.10268413101208215No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG456.769408E-414.7762115
CAAGACG4650.012.4610454
AATCGTA853.9422775E-612.29501513
TCGAATT701.0903423E-412.2154514
TAATACG550.003071606612.0896274
ACGGTAT4800.012.0735289
AAGACGG4800.012.0716385
TCGAACT951.0372769E-612.00302419
TCTACAC3350.011.6230343
GTCTAGT907.5144E-611.6041691
CGACCAT4350.011.57529810
CGGTCCA5750.011.56580310
AGAATTT5900.011.27377517
GACTATA1105.009879E-711.2205611
TGGCGAG1950.011.20820318
GCGCGCT1701.0913936E-1111.17778715
GATATAC1701.2732926E-1111.1697891
ACACGTT600.005866009311.08612719
CGCGTAT951.3600595E-511.0005577
GTGTAAT1303.2971002E-810.9549861
Produced by FastQC (version 0.11.5)