##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062636_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2237931
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206410742779827	32.0	32.0	32.0	32.0	32.0
2	30.90841183217892	32.0	32.0	32.0	32.0	32.0
3	30.898601878252727	32.0	32.0	32.0	32.0	32.0
4	30.954059798983973	32.0	32.0	32.0	32.0	32.0
5	30.782512955046425	32.0	32.0	32.0	32.0	32.0
6	34.43505630870657	36.0	36.0	36.0	32.0	36.0
7	34.32110060587212	36.0	36.0	36.0	32.0	36.0
8	34.32648191566228	36.0	36.0	36.0	32.0	36.0
9	34.45992972973698	36.0	36.0	36.0	32.0	36.0
10	34.173575056603624	36.0	36.0	36.0	32.0	36.0
11	34.446524937542755	36.0	36.0	36.0	32.0	36.0
12	34.24499012704145	36.0	36.0	36.0	32.0	36.0
13	34.36008929676563	36.0	36.0	36.0	32.0	36.0
14	34.277120697644385	36.0	36.0	36.0	32.0	36.0
15	34.225344302393594	36.0	36.0	36.0	32.0	36.0
16	34.22087320833395	36.0	36.0	36.0	32.0	36.0
17	34.14154860002386	36.0	36.0	36.0	32.0	36.0
18	34.16249294549296	36.0	36.0	36.0	32.0	36.0
19	34.14320816861646	36.0	36.0	36.0	32.0	36.0
20	34.13760209765181	36.0	36.0	36.0	32.0	36.0
21	34.10622177359355	36.0	36.0	36.0	32.0	36.0
22	34.068308629712	36.0	36.0	36.0	32.0	36.0
23	34.04564930732896	36.0	36.0	36.0	32.0	36.0
24	34.02183892175407	36.0	36.0	36.0	32.0	36.0
25	33.58469943890138	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	88.0
5	196.0
6	362.0
7	92.0
8	343.0
9	419.0
10	254.0
11	77.0
12	155.0
13	138.0
14	426.0
15	543.0
16	770.0
17	1068.0
18	1419.0
19	1936.0
20	2924.0
21	4120.0
22	6043.0
23	8899.0
24	12387.0
25	17561.0
26	24231.0
27	31799.0
28	42665.0
29	56859.0
30	73962.0
31	101934.0
32	147203.0
33	217806.0
34	504364.0
35	976877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51680374662886	17.16442221968991	11.364923170423797	25.953850863257433
2	17.259444655211936	19.94889744768758	36.3941241895692	26.397533707531277
3	19.403474622244982	23.302258531270976	28.000747153710787	29.293519692773256
4	13.07628297259226	15.110769676298347	35.03867508991713	36.77427226119227
5	15.245140656673453	35.811834962240496	32.78928653642859	16.15373784465746
6	34.56255993133462	34.52290746043135	16.730794591706957	14.183738016527078
7	30.76067331227034	30.028267811806664	20.3786688462959	18.8323900296271
8	28.39378187362225	31.92597068219335	19.59651678302882	20.08373066115558
9	27.367368334424658	14.67213759987405	18.803738769201043	39.15675529650025
10	16.539919786873767	25.951921334767896	30.487743228522408	27.020415649835925
11	37.297763764366515	21.325904714271207	22.09402774279644	19.282303778565833
12	25.206911455588198	23.561910574161953	27.851727103275415	23.379450866974434
13	29.22786888986217	19.13920442217332	25.40432276747308	26.228603920491427
14	23.938027362449557	19.754158501776143	24.531312914396157	31.776501221378144
15	25.451418898485993	26.86351769440403	22.163947685331816	25.521115721778152
16	26.192297472482345	25.57564972797715	23.430959161233815	24.801093638306686
17	24.26051095386884	25.555612224811114	25.0373122308956	25.146564590424447
18	25.226330083534304	24.417787839875437	26.08288106093485	24.27300101565541
19	25.62093035638143	24.83045808197162	25.280302891328965	24.268308670317985
20	25.847192042722988	23.8917841821695	24.920802581560704	25.340221193546814
21	26.98877376754616	23.897007337251843	24.295122264413994	24.819096630788
22	25.968042059249196	24.110586325194824	24.726635465921284	25.194736149634693
23	24.33561120368564	23.87709881172489	25.46571650000268	26.321573484586793
24	24.661502948831586	24.960211691252567	25.064061749844402	25.31422361007144
25	24.943355501943632	24.269751301123225	25.187875392842567	25.599017804090572
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	415.0
1	415.0
2	899.5
3	1384.0
4	1384.0
5	1384.0
6	3301.5
7	5219.0
8	5219.0
9	5219.0
10	5480.5
11	5742.0
12	5742.0
13	5742.0
14	5931.5
15	6121.0
16	6121.0
17	6121.0
18	9983.5
19	13846.0
20	13846.0
21	13846.0
22	22412.0
23	30978.0
24	30978.0
25	30978.0
26	46066.0
27	61154.0
28	61154.0
29	61154.0
30	75165.5
31	89177.0
32	89177.0
33	89177.0
34	111349.5
35	133522.0
36	133522.0
37	133522.0
38	151390.5
39	169259.0
40	169259.0
41	169259.0
42	195108.5
43	220958.0
44	220958.0
45	220958.0
46	256394.0
47	291830.0
48	291830.0
49	291830.0
50	297240.0
51	302650.0
52	302650.0
53	302650.0
54	280671.5
55	258693.0
56	258693.0
57	258693.0
58	239941.0
59	221189.0
60	221189.0
61	221189.0
62	197674.0
63	174159.0
64	174159.0
65	174159.0
66	145016.5
67	115874.0
68	115874.0
69	115874.0
70	88367.5
71	60861.0
72	60861.0
73	60861.0
74	48243.0
75	35625.0
76	35625.0
77	35625.0
78	29284.5
79	22944.0
80	22944.0
81	22944.0
82	16104.5
83	9265.0
84	9265.0
85	9265.0
86	6875.0
87	4485.0
88	4485.0
89	4485.0
90	2959.5
91	1434.0
92	1434.0
93	1434.0
94	844.0
95	254.0
96	254.0
97	254.0
98	573.5
99	893.0
100	893.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008311248202022314
2	0.0034853621492351643
3	0.004691833662431952
4	0.014075500987295854
5	0.03333436106832606
6	0.04450539359792594
7	0.06546225062345533
8	0.0872234219911159
9	0.09450693520041503
10	0.1177873669921012
11	0.1167149478692596
12	0.13034360755537147
13	0.11841294481375877
14	0.12292604195571713
15	0.11327426985014283
16	0.10978890770090767
17	0.12238983239429634
18	0.13052234407584504
19	0.13128197428785784
20	0.12037904653896836
21	0.12949460908312185
22	0.15054083436888804
23	0.1382973827164466
24	0.13306933949259384
25	0.13132665841797625
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2237931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.19054826194316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.77240131207044	34.3680505362826
2	12.583955934066251	11.121838240610412
3	3.789546076959955	5.023863564142687
4	1.599740449083982	2.827736300873133
5	0.8893160898811566	1.9649682795007912
6	0.5662720977739015	1.5014324679641633
7	0.3909904923436097	1.2094658955309838
8	0.310980475694767	1.0993918175769313
9	0.24808543930583388	0.9866728420856838
>10	1.5663693733960673	13.016699603735017
>50	0.13796686774811784	4.2437696677181105
>100	0.11700361310945089	10.859609678384878
>500	0.019261621954220945	5.841973151296652
>1k	0.008008779654649762	5.688805256107216
>5k	1.0137695765379444E-4	0.24572269819071604
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5485	0.24509245369942148	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3730	0.16667180534163029	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3073	0.1373143318538418	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2870	0.1282434534398067	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2804	0.1252943008519923	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2623	0.117206473300562	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2552	0.11403390006215562	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2508	0.11206779833694605	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2444	0.10920801400936847	No Hit
GAACTACGACGGTATCTGATCGTCT	2397	0.1071078598938037	No Hit
GTACATGGAAGCAGTGGTATCAACG	2362	0.1055439153396597	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2354	0.10518644229871252	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2314	0.10339907709397654	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2298	0.10268413101208215	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.936826023679909E-5
2	0.0	0.0	0.0	4.468413011839954E-5	8.936826023679909E-5
3	0.0	0.0	0.0	4.468413011839954E-5	8.936826023679909E-5
4	0.0	0.0	0.0	4.468413011839954E-5	8.936826023679909E-5
5	0.0	0.0	0.0	4.468413011839954E-5	8.936826023679909E-5
6	0.0	0.0	0.0	4.468413011839954E-5	8.936826023679909E-5
7	0.0	0.0	0.0	8.936826023679909E-5	8.936826023679909E-5
8	0.0	0.0	0.0	1.3405239035519861E-4	8.936826023679909E-5
9	0.0	0.0	0.0	2.234206505919977E-4	8.936826023679909E-5
10	0.0	0.0	0.0	2.234206505919977E-4	8.936826023679909E-5
11	0.0	0.0	0.0	2.234206505919977E-4	8.936826023679909E-5
12	0.0	0.0	0.0	2.234206505919977E-4	2.6810478071039723E-4
13	0.0	0.0	0.0	2.234206505919977E-4	3.1278891082879676E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	45	6.769408E-4	14.776211	5
CAAGACG	465	0.0	12.461045	4
AATCGTA	85	3.9422775E-6	12.295015	13
TCGAATT	70	1.0903423E-4	12.21545	14
TAATACG	55	0.0030716066	12.089627	4
ACGGTAT	480	0.0	12.073528	9
AAGACGG	480	0.0	12.071638	5
TCGAACT	95	1.0372769E-6	12.003024	19
TCTACAC	335	0.0	11.623034	3
GTCTAGT	90	7.5144E-6	11.604169	1
CGACCAT	435	0.0	11.575298	10
CGGTCCA	575	0.0	11.565803	10
AGAATTT	590	0.0	11.273775	17
GACTATA	110	5.009879E-7	11.220561	1
TGGCGAG	195	0.0	11.208203	18
GCGCGCT	170	1.0913936E-11	11.177787	15
GATATAC	170	1.2732926E-11	11.169789	1
ACACGTT	60	0.0058660093	11.086127	19
CGCGTAT	95	1.3600595E-5	11.000557	7
GTGTAAT	130	3.2971002E-8	10.954986	1
>>END_MODULE