FastQCFastQC Report
Thu 2 Feb 2017
SRR4062636_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062636_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2237931
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26900.12020031001849477No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26660.11912789089565318No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT26210.1171171050403252No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA25440.11367642702120842No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA24040.10742064880463251No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT22610.10103081819770136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG502.3627526E-630.799815
GTATACG551.592102E-424.004681
GTATCGA500.002581209921.9998659
ACGTCGT708.1266084E-418.85702923
TAGGACG2950.017.8981974
CGCAATA8400.016.76180336
GTATCAA60400.016.7582361
TAGTACG1854.2200554E-1016.6492922
CGTAATC800.001989143416.499913
TATGCGC953.3480625E-416.21042810
GTCCTAA2201.8189894E-1116.003121
TTAGGAC3350.015.761453
CTAGCGG9100.015.714190529
AACGTCG850.002981121715.52931822
ATACGAA9200.015.30425540
CGGTCCA9700.015.19578410
GGCGTTA7100.015.18300642
CGTCGTA7550.015.15222610
GCCGGTC9150.015.1474498
TAGCGGC9450.015.13218330
Produced by FastQC (version 0.11.5)