FastQCFastQC Report
Thu 2 Feb 2017
SRR4062635_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062635_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864841
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT32710.37821981150292366No Hit
GTCCTACAGTGGACATTTCTAAATT29920.34595954632123127No Hit
CTGTAGGACGTGGAATATGGCAAGA28250.32664963848846207No Hit
CTTTAGGACGTGAAATATGGCGAGG26270.30375525674661585No Hit
GATATACACTGTTCTACAAATCCCG22820.2638635309843081No Hit
TTGTAGAACAGTGTATATCAATGAG20580.23796281628646188No Hit
GTATCAACGCAGAGTACTTTTTTTT20580.23796281628646188No Hit
GTCCTACAGTGTGCATTTCTCATTT18560.21460592178215418No Hit
GTACATGGAAGCAGTGGTATCAACG18060.20882451225138493No Hit
GTGTATATCAATGAGTTACAATGAG16940.19587415490246182No Hit
GTTCTACAGTGTGGTTTTTATCATT15720.18176751564738491No Hit
GTGTATATCAATGAGTTACAATGAA15670.18118937469430796No Hit
TCCATGTACTCTGCGTTGATACCAC15570.18003309278815413No Hit
TATCAACGCAGAGTACTTTTTTTTT13590.15713871104630792No Hit
CTGAAGGACCTGGAATATGGCGAGA13520.15632931371200023No Hit
GTATTAGAGGCACTGCCTGCCCAGT13310.15390112170907716No Hit
GTCCTTCAGTGTGCATTTCTCATTT13290.15366986532784638No Hit
CTGTAGGACCTGGAATATGGCGAGA12980.15008539141876948No Hit
ATTTAGAAATGTCCACTGTAGGACG12530.14488212284107715No Hit
GTTCTACAAATCCCGTTTCCAACGA11570.13378181654200022No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11130.12869417615492326No Hit
CCATAGGGTCTTCTCGTCTTATTAT11010.12730663786753865No Hit
GATATACACTGTTCTACAATGCCGG10530.12175648471800019No Hit
GAGTACTTTTTTTTTTTTTTTTTTT10090.11666884433092325No Hit
CTCTAATACTTGTAATGCTAGAGGT9820.11354688318430788No Hit
TTTCTAAATTTTCCACCTTTTTCAG9730.1125062294687694No Hit
GAATATGGCAAGAAAACTGAAAATC9380.10845924279723095No Hit
ACGCAGAGTACTTTTTTTTTTTTTT9240.10684044812861555No Hit
CCATTGGGATGTCCTGATCCAACAT9060.10475914069753861No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCG307.7170314E-419.0006949
GGACGTT250.006028680619.0006946
CTTGCGG508.6826E-515.20495916
TTGCGGT508.6826E-515.20495917
CCGAAAT456.73837E-414.78345518
CGGTTGG400.00526315814.25547518
TAGGGGT400.005281392514.2480474
TGCGGTA551.952192E-413.8234918
CCTTGCG551.9579563E-413.81868815
ACTCGGC707.25087E-613.57192511
TAGGACC5100.013.4099254
CGAAATT500.001494274213.30510919
CTACCCT500.001500155913.2981764
CCGAGGT500.001502776413.2950973
CGTCTTA1650.012.667129515
TAAGCTG751.4779855E-512.6656635
TCCGAGG604.1055007E-412.6605322
GTCCTAT1052.00871E-812.6576011
GGCGAGG6800.012.5783619
ACTGTTC8000.012.4692068
Produced by FastQC (version 0.11.5)