##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062635_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	864841
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.155349942937487	32.0	32.0	32.0	32.0	32.0
2	30.88117237735029	32.0	32.0	32.0	32.0	32.0
3	30.89667002373847	32.0	32.0	32.0	32.0	32.0
4	30.8116532403066	32.0	32.0	32.0	32.0	32.0
5	30.83160372831538	32.0	32.0	32.0	32.0	32.0
6	34.31787924023028	36.0	36.0	36.0	32.0	36.0
7	34.21501293301312	36.0	36.0	36.0	32.0	36.0
8	34.07875089178242	36.0	36.0	36.0	32.0	36.0
9	34.35137672705156	36.0	36.0	36.0	32.0	36.0
10	34.030289960813604	36.0	36.0	36.0	32.0	36.0
11	34.367625956678744	36.0	36.0	36.0	32.0	36.0
12	34.15803714208739	36.0	36.0	36.0	32.0	36.0
13	34.22417531083748	36.0	36.0	36.0	32.0	36.0
14	34.14292106872824	36.0	36.0	36.0	32.0	36.0
15	34.11309130811328	36.0	36.0	36.0	32.0	36.0
16	34.23338856506572	36.0	36.0	36.0	32.0	36.0
17	34.107960885295675	36.0	36.0	36.0	32.0	36.0
18	34.19659336224809	36.0	36.0	36.0	32.0	36.0
19	34.136922278199116	36.0	36.0	36.0	32.0	36.0
20	33.98811457828664	36.0	36.0	36.0	32.0	36.0
21	33.97867931793243	36.0	36.0	36.0	32.0	36.0
22	33.969081021829446	36.0	36.0	36.0	32.0	36.0
23	33.886949161753435	36.0	36.0	36.0	32.0	36.0
24	33.854155850613004	36.0	36.0	36.0	32.0	36.0
25	33.22909413406626	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	31.0
5	83.0
6	142.0
7	41.0
8	143.0
9	160.0
10	109.0
11	29.0
12	71.0
13	47.0
14	229.0
15	384.0
16	668.0
17	926.0
18	1088.0
19	1375.0
20	1661.0
21	1980.0
22	2431.0
23	3069.0
24	4189.0
25	5641.0
26	7943.0
27	10479.0
28	14862.0
29	20830.0
30	29074.0
31	42992.0
32	67127.0
33	103161.0
34	229450.0
35	314423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.99635620180005	20.29402479540495	11.895596103876581	24.814022898918424
2	13.102525618106931	22.27971665714636	44.00717838897895	20.610579335767756
3	18.793232239369292	26.799536543455986	31.09425697786556	23.312974239309163
4	10.450957830036486	18.16552274448807	41.214272910727814	30.169246514747634
5	10.612183995262242	40.53547303549617	37.28636136506644	11.56598160417515
6	28.10191451211753	40.722308982590086	19.593845797906184	11.5819307073862
7	25.09904747799202	33.67151413046497	23.125966175749106	18.103472215793907
8	22.96793083893666	39.994560625875216	21.560753179719235	15.476755355468885
9	26.467449553347176	15.164552039278595	21.186321011317368	37.18167739605686
10	13.542677209108483	30.45588729002906	36.41472082981211	19.586714671050345
11	33.73381833000303	22.58047710891355	26.6432889627755	17.042415598307915
12	22.31235302767335	26.253613883797644	34.22100469732529	17.213028391203718
13	28.275355876006035	23.30848915932687	27.420280996174967	20.995873968492123
14	19.250788387891475	24.76024209644033	28.75832959013091	27.230639925537286
15	21.69712376626173	34.12804252649224	24.808586795209287	19.366246912036747
16	20.061399335303577	29.287853373201227	31.021713184997914	19.629034106497286
17	18.902435494757267	29.771686114426693	30.620641909218577	20.705236481597467
18	18.71420863600892	28.926947988041952	34.035401754804404	18.32344162114472
19	22.421173166052448	27.51717326794003	28.629021520278524	21.432632045729
20	21.659276410998554	30.001041968162085	30.13661360347323	18.203068017366135
21	22.069468565474125	27.682644436725717	27.607502605071204	22.640384392728958
22	20.926270979367175	31.336599782056613	28.409881798716196	19.327247439860017
23	20.706540303018024	30.343486038823826	28.871700756676645	20.078272901481512
24	21.64588326559913	29.25214491646115	29.75673579029027	19.34523602764945
25	21.115256011263014	29.53403449297914	30.226163350746543	19.1245461450113
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	107.0
2	394.5
3	682.0
4	682.0
5	682.0
6	1837.5
7	2993.0
8	2993.0
9	2993.0
10	3347.5
11	3702.0
12	3702.0
13	3702.0
14	4589.0
15	5476.0
16	5476.0
17	5476.0
18	8992.0
19	12508.0
20	12508.0
21	12508.0
22	20875.0
23	29242.0
24	29242.0
25	29242.0
26	45017.0
27	60792.0
28	60792.0
29	60792.0
30	79874.0
31	98956.0
32	98956.0
33	98956.0
34	103930.5
35	108905.0
36	108905.0
37	108905.0
38	108078.5
39	107252.0
40	107252.0
41	107252.0
42	106301.0
43	105350.0
44	105350.0
45	105350.0
46	109850.5
47	114351.0
48	114351.0
49	114351.0
50	101032.5
51	87714.0
52	87714.0
53	87714.0
54	71470.0
55	55226.0
56	55226.0
57	55226.0
58	43999.5
59	32773.0
60	32773.0
61	32773.0
62	25392.0
63	18011.0
64	18011.0
65	18011.0
66	14120.5
67	10230.0
68	10230.0
69	10230.0
70	7695.0
71	5160.0
72	5160.0
73	5160.0
74	4011.0
75	2862.0
76	2862.0
77	2862.0
78	2038.5
79	1215.0
80	1215.0
81	1215.0
82	928.0
83	641.0
84	641.0
85	641.0
86	450.0
87	259.0
88	259.0
89	259.0
90	171.5
91	84.0
92	84.0
93	84.0
94	52.0
95	20.0
96	20.0
97	20.0
98	175.0
99	330.0
100	330.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009712768011692323
2	0.0026594483841538502
3	0.004046986671538468
4	0.014569152017538485
5	0.03434157261276928
6	0.04521062253061545
7	0.07041756808476933
8	0.08914933496446167
9	0.09932461573861555
10	0.11921266452446172
11	0.12198774109923095
12	0.13389744473261558
13	0.12221899748046172
14	0.12175648471800019
15	0.11597507518723094
16	0.11424065232800017
17	0.12418467672092327
18	0.13355056016076944
19	0.1322786500640002
20	0.1261503559613848
21	0.13193176549215405
22	0.15193544246861562
23	0.1382913159760002
24	0.1354006112106156
25	0.13054422720476944
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	864841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.02017277541405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.97801996589985	44.82486975119666
2	11.34756313357227	12.25994642110986
3	2.6671930613975916	4.322466900062503
4	0.9428932677050935	2.0374102892081556
5	0.43263646059494315	1.168554817514122
6	0.27045632398923913	0.8766058410061235
7	0.1817346462652263	0.6872135893368397
8	0.14027789447995698	0.6062268877102862
9	0.10176437580958143	0.494759624725418
>10	0.6965483897600677	7.477860695224246
>50	0.11391383224596077	4.314610847516527
>100	0.11048024301785551	12.128927221674006
>500	0.011369811414369234	3.9889339382045774
>1k	0.005148593848016257	4.811613175510708
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	3271	0.37821981150292366	No Hit
GTCCTACAGTGGACATTTCTAAATT	2992	0.34595954632123127	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2825	0.32664963848846207	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2627	0.30375525674661585	No Hit
GATATACACTGTTCTACAAATCCCG	2282	0.2638635309843081	No Hit
TTGTAGAACAGTGTATATCAATGAG	2058	0.23796281628646188	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2058	0.23796281628646188	No Hit
GTCCTACAGTGTGCATTTCTCATTT	1856	0.21460592178215418	No Hit
GTACATGGAAGCAGTGGTATCAACG	1806	0.20882451225138493	No Hit
GTGTATATCAATGAGTTACAATGAG	1694	0.19587415490246182	No Hit
GTTCTACAGTGTGGTTTTTATCATT	1572	0.18176751564738491	No Hit
GTGTATATCAATGAGTTACAATGAA	1567	0.18118937469430796	No Hit
TCCATGTACTCTGCGTTGATACCAC	1557	0.18003309278815413	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1359	0.15713871104630792	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1352	0.15632931371200023	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1331	0.15390112170907716	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1329	0.15366986532784638	No Hit
CTGTAGGACCTGGAATATGGCGAGA	1298	0.15008539141876948	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1253	0.14488212284107715	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1157	0.13378181654200022	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1113	0.12869417615492326	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1101	0.12730663786753865	No Hit
GATATACACTGTTCTACAATGCCGG	1053	0.12175648471800019	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1009	0.11666884433092325	No Hit
CTCTAATACTTGTAATGCTAGAGGT	982	0.11354688318430788	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	973	0.1125062294687694	No Hit
GAATATGGCAAGAAAACTGAAAATC	938	0.10845924279723095	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	924	0.10684044812861555	No Hit
CCATTGGGATGTCCTGATCCAACAT	906	0.10475914069753861	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3125638123076958E-4	0.0
10	0.0	0.0	0.0	2.3125638123076958E-4	0.0
11	0.0	0.0	0.0	2.3125638123076958E-4	0.0
12	0.0	0.0	0.0	2.3125638123076958E-4	0.0
13	0.0	0.0	0.0	2.3125638123076958E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCACTCG	30	7.7170314E-4	19.000694	9
GGACGTT	25	0.0060286806	19.000694	6
CTTGCGG	50	8.6826E-5	15.204959	16
TTGCGGT	50	8.6826E-5	15.204959	17
CCGAAAT	45	6.73837E-4	14.783455	18
CGGTTGG	40	0.005263158	14.255475	18
TAGGGGT	40	0.0052813925	14.248047	4
TGCGGTA	55	1.952192E-4	13.82349	18
CCTTGCG	55	1.9579563E-4	13.818688	15
ACTCGGC	70	7.25087E-6	13.571925	11
TAGGACC	510	0.0	13.409925	4
CGAAATT	50	0.0014942742	13.305109	19
CTACCCT	50	0.0015001559	13.298176	4
CCGAGGT	50	0.0015027764	13.295097	3
CGTCTTA	165	0.0	12.6671295	15
TAAGCTG	75	1.4779855E-5	12.665663	5
TCCGAGG	60	4.1055007E-4	12.660532	2
GTCCTAT	105	2.00871E-8	12.657601	1
GGCGAGG	680	0.0	12.57836	19
ACTGTTC	800	0.0	12.469206	8
>>END_MODULE