FastQCFastQC Report
Thu 2 Feb 2017
SRR4062635_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062635_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences864841
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15550.17980183640692335No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG13700.15841062114307716No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA12880.14892910951261562No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT12760.14754157122523098No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC12420.1436102127443079No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC11770.1360943803543079No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA11560.13366618835138483No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG10960.12672849691446172No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC8680.100365269454154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCA1950.022.56407413
CGCAAGG1900.021.99997116
ATACACT11400.021.806994
ACCGCAA1950.021.43587114
AAAGGCG1158.762072E-921.043455
ACTGTTC11000.020.9999718
CCGCAAG2000.020.89997315
TAGGACC5500.020.7999734
GATATAC12350.020.6651341
TAGAACG755.470382E-520.5333064
CGCGGTA3350.020.35818343
GCGGTAC550.00448263219.99997329
CGCGCTT550.00448263219.99997312
TGCGGTA550.00448263219.99997328
TTGCGGT550.00448263219.99997327
GACGTGA12050.019.5352467
CCGCGGT3500.019.4856942
TTAGGAC12750.019.3254643
TGTAGGA19600.019.1938532
CTGTAGG19500.019.0677431
Produced by FastQC (version 0.11.5)