##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062635_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	864841
Sequences flagged as poor quality	0
Sequence length	50
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.172942772139617	32.0	32.0	32.0	32.0	32.0
2	31.276775731030327	32.0	32.0	32.0	32.0	32.0
3	31.419207692512266	32.0	32.0	32.0	32.0	32.0
4	31.549710293568413	32.0	32.0	32.0	32.0	32.0
5	31.469690960534944	32.0	32.0	32.0	32.0	32.0
6	35.05846739458467	36.0	36.0	36.0	36.0	36.0
7	35.073655157422	36.0	36.0	36.0	36.0	36.0
8	35.00180033092788	36.0	36.0	36.0	36.0	36.0
9	35.17142226143303	36.0	36.0	36.0	36.0	36.0
10	35.027912645214556	36.0	36.0	36.0	36.0	36.0
11	35.17364810410237	36.0	36.0	36.0	36.0	36.0
12	35.10087634605667	36.0	36.0	36.0	36.0	36.0
13	35.13518901162179	36.0	36.0	36.0	36.0	36.0
14	35.104547541108715	36.0	36.0	36.0	36.0	36.0
15	35.055392840996205	36.0	36.0	36.0	36.0	36.0
16	35.087047214459076	36.0	36.0	36.0	36.0	36.0
17	35.08618925328471	36.0	36.0	36.0	36.0	36.0
18	35.09204697742128	36.0	36.0	36.0	36.0	36.0
19	35.06568490624288	36.0	36.0	36.0	36.0	36.0
20	35.03589561549464	36.0	36.0	36.0	36.0	36.0
21	35.016111632080346	36.0	36.0	36.0	36.0	36.0
22	35.009213254228236	36.0	36.0	36.0	36.0	36.0
23	34.96183113427786	36.0	36.0	36.0	36.0	36.0
24	34.960388094458985	36.0	36.0	36.0	36.0	36.0
25	34.94399779843925	36.0	36.0	36.0	36.0	36.0
26	34.89785521269228	36.0	36.0	36.0	32.0	36.0
27	34.86980034480327	36.0	36.0	36.0	32.0	36.0
28	34.83862235948573	36.0	36.0	36.0	32.0	36.0
29	34.80694948551237	36.0	36.0	36.0	32.0	36.0
30	34.77764005175518	36.0	36.0	36.0	32.0	36.0
31	34.77225524691822	36.0	36.0	36.0	32.0	36.0
32	34.73610640568613	36.0	36.0	36.0	32.0	36.0
33	34.73631800527496	36.0	36.0	36.0	32.0	36.0
34	34.70927950918146	36.0	36.0	36.0	32.0	36.0
35	34.67725512550862	36.0	36.0	36.0	32.0	36.0
36	34.63120966744176	36.0	36.0	36.0	32.0	36.0
37	34.59732598246383	36.0	36.0	36.0	32.0	36.0
38	34.55417585428998	36.0	36.0	36.0	32.0	36.0
39	34.4863495139569	36.0	36.0	36.0	32.0	36.0
40	34.497185031699466	36.0	36.0	36.0	32.0	36.0
41	34.46318918737664	36.0	36.0	36.0	32.0	36.0
42	34.43335480163406	36.0	36.0	36.0	32.0	36.0
43	34.41583019306439	36.0	36.0	36.0	32.0	36.0
44	34.385548326224125	36.0	36.0	36.0	32.0	36.0
45	34.330301176748094	36.0	36.0	36.0	32.0	36.0
46	34.2709839149624	36.0	36.0	36.0	32.0	36.0
47	34.26978138178001	36.0	36.0	36.0	32.0	36.0
48	34.20176888006004	36.0	36.0	36.0	32.0	36.0
49	34.182032304203894	36.0	36.0	36.0	32.0	36.0
50	33.67098345244964	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	5.0
21	22.0
22	59.0
23	166.0
24	434.0
25	1133.0
26	2467.0
27	4910.0
28	8877.0
29	13865.0
30	20981.0
31	30877.0
32	46220.0
33	76227.0
34	164565.0
35	494033.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.18035348144413	20.814304861844334	12.206593401510096	24.798748255201435
2	12.792870506774962	22.729017740477012	43.759612026799196	20.71849972594883
3	18.576080144489488	27.10727326967594	31.47433392149744	22.842312664337136
4	10.522986910873687	18.6648397391425	41.49380232181675	29.31837102816706
5	10.751109163418478	40.85398356460899	36.7963590995339	11.598548172438633
6	28.240978956776924	40.87523805008794	19.52259322255356	11.361189770581577
7	25.285399941492	33.898448150465	22.975258978838838	17.840892929204163
8	23.081583782452498	40.034064064955295	21.520256324572955	15.364095828019256
9	26.53967607918681	15.087166311495409	21.123189117999726	37.24996849131806
10	13.595447024366328	30.540873987241586	36.32112723610467	19.542551752287416
11	33.863218788193436	22.557325566202344	26.60766545526866	16.971790190335565
12	22.412905956123726	26.23198946395927	34.08106229931282	17.274042280604178
13	28.48442661714697	23.178595834378804	27.4272380703505	20.909739478123722
14	19.355349711681107	24.773339839346193	28.494139385158658	27.377171063814043
15	21.8242428377008	34.08580305512805	24.738188869399114	19.351765237772028
16	20.198510477648494	29.442984317348508	30.726225976798048	19.632279228204954
17	19.026734393952182	29.769980840408817	30.306611273054816	20.896673492584185
18	18.92833480373849	29.11194080761666	33.58952686100682	18.370197527638027
19	22.731692877650342	27.63398127517081	28.370532849390813	21.263792997788034
20	21.961609128151878	30.26868522653297	29.614576552221738	18.15512909309341
21	22.386311472282188	27.746371876448965	27.30432530372635	22.562991347542496
22	21.116135798372188	31.319398594654974	28.170496079626194	19.393969527346645
23	21.020742541114494	30.33332138508697	28.581323040882662	20.064613032915876
24	21.915126595524494	29.325737332064506	29.286192490874047	19.472943581536953
25	21.58905509798911	29.505770424852663	29.67539698048543	19.2297774966728
26	18.13339099325772	30.43148971891943	31.603728315378206	19.831390972444645
27	19.719462883928955	30.452649677802047	30.29712976142435	19.530757676844644
28	18.983026937899567	31.164688075611586	28.722273805242814	21.130011181246033
29	20.476018135125415	28.659834582310506	28.455403941302503	22.408743341261573
30	20.07432580092757	29.55780311062958	29.326199844826967	21.041671243615877
31	21.037971141516188	28.284505475572967	29.504382886565278	21.17314049634557
32	19.3861068103848	30.618344874953895	29.73402047312743	20.261527841533876
33	19.76918300589357	30.17063252089112	30.597184916071278	19.46299955714403
34	19.804912116793723	30.326614950031278	31.2970823538662	18.571390579308797
35	20.008070847704953	30.768430266372665	29.725926499784354	19.497572386138028
36	19.055294557034184	29.945388805572353	30.5740592779482	20.42525735944526
37	19.66743019815203	29.789753261004048	28.377239284446503	22.165577256397416
38	20.017205474763568	30.98384558548912	28.614623959779887	20.384324979967417
39	20.889504544766034	28.54524704541066	29.2663044420882	21.29894396773511
40	22.584845075568804	28.97966215755266	29.595960413532662	18.839532353345874
41	21.209332120008188	27.184303241867582	31.833481530130975	19.772883107993263
42	20.408722528187262	30.751895435114662	29.739801882658202	19.099580154039874
43	18.35088761980526	30.505260504532046	31.006624339040357	20.137227536622337
44	18.54548986461095	30.034769396918048	30.393910556969434	21.02583018150157
45	18.9050935374248	30.32083354050051	30.378763263998813	20.395309658075877
46	20.15075603492434	28.114647663558966	29.3474754319002	22.387120869616496
47	21.396534160614493	28.632777585706503	29.314868282146662	20.65581997153234
48	19.027196906714643	29.0814149652942	29.157729571100354	22.733658556890806
49	20.106354809728032	28.32555348324143	30.459934253810815	21.108157453219725
50	18.7923560515748	30.53220187294543	28.665500363650658	22.00994171182911
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14.0
1	14.0
2	14.0
3	34.0
4	54.0
5	73.0
6	92.0
7	95.5
8	99.0
9	176.0
10	253.0
11	343.5
12	434.0
13	763.5
14	1093.0
15	1526.5
16	1960.0
17	2726.5
18	3493.0
19	4138.5
20	4784.0
21	6235.5
22	7687.0
23	8331.0
24	8975.0
25	12205.5
26	15436.0
27	21840.0
28	28244.0
29	33455.5
30	38667.0
31	41947.0
32	45227.0
33	53350.5
34	61474.0
35	71433.0
36	81392.0
37	83526.0
38	85660.0
39	80033.0
40	74406.0
41	69964.5
42	65523.0
43	60021.5
44	54520.0
45	53119.5
46	51719.0
47	50108.0
48	48497.0
49	44385.5
50	40274.0
51	37709.0
52	35144.0
53	31389.0
54	27634.0
55	24685.0
56	21736.0
57	20022.0
58	18308.0
59	15319.5
60	12331.0
61	10416.0
62	8501.0
63	7304.5
64	6108.0
65	5292.0
66	4476.0
67	3877.5
68	3279.0
69	2855.0
70	2431.0
71	2085.5
72	1740.0
73	1462.5
74	1185.0
75	993.5
76	802.0
77	625.0
78	448.0
79	403.0
80	358.0
81	268.5
82	179.0
83	142.0
84	105.0
85	75.5
86	46.0
87	33.0
88	20.0
89	14.5
90	9.0
91	6.0
92	3.0
93	3.5
94	4.0
95	3.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014800408398769253
2	0.004509499434000006
3	3.4688457184615435E-4
4	1.1562819061538479E-4
5	0.0
6	2.3125638123076958E-4
7	2.3125638123076958E-4
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	864841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.6488461634727
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.42867915079687	53.73788011054265
2	10.678078383113045	13.592947366565308
3	2.419246799106656	4.6194680204343985
4	0.8261322202260332	2.1032945038341975
5	0.39675325449501136	1.2626443430105054
6	0.24097355745251017	0.9202613332655741
7	0.14587495137238488	0.649934063730349
8	0.11922831636922424	0.6070995813531687
9	0.08610381427061119	0.49323675857385213
>10	0.5088352505984383	6.404720931380194
>50	0.07468383364216452	3.3303497916917952
>100	0.06920234284317618	9.114718534250137
>500	0.0047473902517758395	1.9826732521284811
>1k	0.0014607354620848735	1.1807714092394705
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1555	0.17980183640692335	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	1370	0.15841062114307716	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1288	0.14892910951261562	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	1276	0.14754157122523098	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1242	0.1436102127443079	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	1177	0.1360943803543079	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1156	0.13366618835138483	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	1096	0.12672849691446172	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	868	0.100365269454154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1562819061538479E-4	0.0
5	0.0	0.0	0.0	1.1562819061538479E-4	0.0
6	0.0	0.0	0.0	1.1562819061538479E-4	0.0
7	0.0	0.0	0.0	1.1562819061538479E-4	0.0
8	0.0	0.0	0.0	1.1562819061538479E-4	0.0
9	0.0	0.0	0.0	3.4688457184615435E-4	0.0
10	0.0	0.0	0.0	3.4688457184615435E-4	0.0
11	0.0	0.0	0.0	3.4688457184615435E-4	0.0
12	0.0	0.0	0.0	3.4688457184615435E-4	0.0
13	0.0	0.0	0.0	3.4688457184615435E-4	0.0
14	0.0	0.0	0.0	3.4688457184615435E-4	0.0
15	0.0	0.0	0.0	3.4688457184615435E-4	0.0
16	0.0	0.0	0.0	3.4688457184615435E-4	0.0
17	0.0	0.0	0.0	3.4688457184615435E-4	0.0
18	0.0	0.0	0.0	4.6251276246153916E-4	0.0
19	0.0	0.0	0.0	4.6251276246153916E-4	0.0
20	0.0	0.0	0.0	9.250255249230783E-4	0.0
21	0.0	0.0	0.0	0.0010406537155384632	0.0
22	0.0	0.0	0.0	0.0019656792404615414	0.0
23	0.0	0.0	0.0	0.0034688457184615436	0.0
24	0.0	0.0	0.0	0.004625127624615392	0.0
25	0.0	0.0	0.0	0.0053188967683077005	0.0
26	0.0	0.0	0.0	0.007053319627538472	0.0
27	0.0	0.0	0.0	0.008093973343076936	0.0
28	0.0	0.0	0.0	0.013065985539538482	0.0
29	0.0	0.0	0.0	0.024281920029230807	0.0
30	0.0	0.0	0.0	0.047060673580461614	0.0
31	1.1562819061538479E-4	0.0	0.0	0.10499039707876939	0.0
32	1.1562819061538479E-4	0.0	0.0	0.18581450231892335	0.0
33	1.1562819061538479E-4	0.0	0.0	0.274963837283385	0.0
34	1.1562819061538479E-4	0.0	0.0	0.3800698625527698	0.0
35	1.1562819061538479E-4	0.0	0.0	0.49246046383092384	0.0
36	1.1562819061538479E-4	0.0	0.0	0.6530680205956934	0.0
37	1.1562819061538479E-4	0.0	0.0	0.904790591565386	0.0
38	1.1562819061538479E-4	0.0	0.0	1.2155991679395404	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCGCA	195	0.0	22.564074	13
CGCAAGG	190	0.0	21.999971	16
ATACACT	1140	0.0	21.80699	4
ACCGCAA	195	0.0	21.435871	14
AAAGGCG	115	8.762072E-9	21.04345	5
ACTGTTC	1100	0.0	20.999971	8
CCGCAAG	200	0.0	20.899973	15
TAGGACC	550	0.0	20.799973	4
GATATAC	1235	0.0	20.665134	1
TAGAACG	75	5.470382E-5	20.533306	4
CGCGGTA	335	0.0	20.358183	43
GCGGTAC	55	0.004482632	19.999973	29
CGCGCTT	55	0.004482632	19.999973	12
TGCGGTA	55	0.004482632	19.999973	28
TTGCGGT	55	0.004482632	19.999973	27
GACGTGA	1205	0.0	19.535246	7
CCGCGGT	350	0.0	19.48569	42
TTAGGAC	1275	0.0	19.325464	3
TGTAGGA	1960	0.0	19.193853	2
CTGTAGG	1950	0.0	19.067743	1
>>END_MODULE