FastQCFastQC Report
Thu 2 Feb 2017
SRR4062634_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062634_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651508
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT42550.6531001921695512No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35460.544275741817445No Hit
TATCAACGCAGAGTACTTTTTTTTT27150.4167255045218171No Hit
GTACATGGAAGCAGTGGTATCAACG26840.4119673127574796No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26320.4039858297979457No Hit
TCCATGTACTCTGCGTTGATACCAC22320.34258980703230046No Hit
ACGCAGAGTACTTTTTTTTTTTTTT18190.27919841352677177No Hit
GCGCAAGACGGACCAGAGCGAAAGC15350.23560723736316364No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14920.22900716491585674No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14090.21626749019198538No Hit
GAATAGGACCGCGGTTCTATTTTGT14000.21488607967975834No Hit
GATTAAGAGGGACGGCCGGGGGCAT13830.21227674871221844No Hit
GAACTACGACGGTATCTGATCGTCT13550.20797902711862326No Hit
GAATAACGCCGCCGCATCGCCAGTC13550.20797902711862326No Hit
GTATCTGATCGTCTTCGAACCTCCG13280.2038347955819422No Hit
GGTATCAACGCAGAGTACTTTTTTT13240.20322083535428576No Hit
GTACATGGGGTGGTATCAACGCAAA12950.19876962370377646No Hit
GTCCTATTCCATTATTCCTAGCTGC12820.196774252963893No Hit
GAGTACATGGAAGCAGTGGTATCAA12070.1852624986953345No Hit
CTCTTAATCATGGCCTCAGTTCCGA11700.17958336658951232No Hit
CTATTGGAGCTGGAATTACCGCGGC11010.16899255266243854No Hit
ATCAGATACCGTCGTAGTTCCGACC10660.16362040067044456No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG10530.1616250299305611No Hit
GAATAATGGAATAGGACCGCGGTTC10380.1593226790768494No Hit
CATGTACTCTGCGTTGATACCACTG10280.15778777850770825No Hit
CTTTAATATACGCTATTGGAGCTGG10160.15594589782473892No Hit
TCGTAGTTCCGACCATAAACGATGC9660.14827139497903327No Hit
GCTTTGAACACTCTAATTTTTTCAA9370.14382018332852398No Hit
GGGTAGGCACACGCTGAGCCAGTCA9280.14243877281629697No Hit
GCAGAGTACTTTTTTTTTTTTTTTT9220.14151783247481228No Hit
GTGCATGGCCGTTCTTAGTTGGTGG9060.13906199156418647No Hit
ATATTAAAGTTGCTGCAGTTAAAAA8670.13307587934453607No Hit
CCATTATTCCTAGCTGCGGTATCCA8630.1324619191168796No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC8500.13046654837699614No Hit
GTCCTAAAGTGTGTATTTCTCATTT8450.12969909809242558No Hit
GTACATGGGGAATAATTGCAATCCC8360.12831768758019857No Hit
GTCCTACAGTGGACATTTCTAAATT8210.12601533672648688No Hit
GTCCTGTATTGTTATTTTTCGTCAC8210.12601533672648688No Hit
GTACATGGGTGGTATCAACGCAAAA8160.1252478864419163No Hit
ATACAGGACTCTTTCGAGGCCCTGT8150.12509439638500217No Hit
GTTCTATTTTGTTGGTTTTCGGAAC8130.12478741627117394No Hit
GAATAATTGCAATCCCCGATCCCCA8080.12401996598660339No Hit
CTTTAAATCCTTTAACGAGGATCCA7780.11941526427918No Hit
CTGTAGGACGTGGAATATGGCAAGA7650.11741989353929654No Hit
CTTTAGGACGTGAAATATGGCGAGG7390.11342915205952958No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT7380.11327566200261548No Hit
CTCCCGACCCGGGGAGGTAGTGACG7340.11266170177495902No Hit
GCTAAGAGCATCGAGGGGGCGCCGA7280.11174076143347433No Hit
CTCCAATAGCGTATATTAAAGTTGC7150.10974539069359088No Hit
CATCTAAGGGCATCACAGACCTGTT6910.10606162932765215No Hit
ACCATACTCCCCCCGGAACCCAAAG6900.10590813927073804No Hit
GCTTCCATGTACTCTGCGTTGATAC6880.1056011591569098No Hit
GGTCGGGAGTGGGTAATTTGCGCGC6870.1054476690999957No Hit
GCCATGCACCACCACCCACGGAATC6700.10283833813245577No Hit
GTATCAACGCAGAGTACATGGGGTG6670.10237786796171344No Hit
GGATTGACAGATTGATAGCTCTTTC6650.1020708878478852No Hit
GCGTTGATACCACTGCTTCCATGTA6570.10084296739257231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC802.1827873E-1117.81293310
TAACGAA907.2759576E-1216.89059613
ATGTACG350.002166912716.28736117
TCCGTTG350.002168025616.2861112
TACGCCT350.002169138816.2848595
GTACAGG350.002173596516.2798541
GTTTAAA601.4691177E-615.8276371
ACGAACG953.0377123E-1015.00151615
TTATACT456.757554E-414.7774
ATCGCCA2050.014.36730716
GATAACG1006.566552E-1014.25034511
CCGATAA1006.566552E-1014.2503459
ATAACGA1006.566552E-1014.25034512
CAATTAG602.5724534E-514.2448731
GGATAAA400.00528735114.2448731
CCATTAA400.00528735114.2448731
CATCGCC2100.014.02522815
TCGCCAG2050.013.90384617
CGAACGA1051.3624231E-913.57280116
AACCGCG1400.013.5717587
Produced by FastQC (version 0.11.5)