##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062634_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651508
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.840293595780864	32.0	32.0	32.0	32.0	32.0
2	30.596029519207747	32.0	32.0	32.0	32.0	32.0
3	30.620672347845307	32.0	32.0	32.0	32.0	32.0
4	30.61610755355268	32.0	32.0	32.0	32.0	32.0
5	30.50527545325614	32.0	32.0	32.0	32.0	32.0
6	34.0718732540506	36.0	36.0	36.0	32.0	36.0
7	33.84672175936443	36.0	36.0	36.0	32.0	36.0
8	33.822135722047925	36.0	36.0	36.0	32.0	36.0
9	33.91473627338421	36.0	36.0	36.0	32.0	36.0
10	33.659740172031654	36.0	36.0	36.0	27.0	36.0
11	34.050587252957754	36.0	36.0	36.0	32.0	36.0
12	33.7894530842292	36.0	36.0	36.0	32.0	36.0
13	33.948324502538725	36.0	36.0	36.0	32.0	36.0
14	33.86573917741609	36.0	36.0	36.0	32.0	36.0
15	33.831965225292706	36.0	36.0	36.0	32.0	36.0
16	33.807102905873755	36.0	36.0	36.0	32.0	36.0
17	33.70710873849592	36.0	36.0	36.0	27.0	36.0
18	33.776460150911426	36.0	36.0	36.0	32.0	36.0
19	33.71195134979156	36.0	36.0	36.0	27.0	36.0
20	33.68518421876631	36.0	36.0	36.0	27.0	36.0
21	33.61639611485968	36.0	36.0	36.0	27.0	36.0
22	33.58534200654481	36.0	36.0	36.0	27.0	36.0
23	33.6059050694696	36.0	36.0	36.0	27.0	36.0
24	33.61034400191556	36.0	36.0	36.0	27.0	36.0
25	33.16901250636984	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	25.0
5	58.0
6	102.0
7	36.0
8	90.0
9	126.0
10	80.0
11	22.0
12	48.0
13	46.0
14	341.0
15	348.0
16	422.0
17	633.0
18	819.0
19	1175.0
20	1738.0
21	2435.0
22	3444.0
23	4530.0
24	5771.0
25	7451.0
26	9547.0
27	11722.0
28	15251.0
29	19588.0
30	24587.0
31	32835.0
32	46601.0
33	66163.0
34	144024.0
35	251447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.94040742520228	18.46445736249668	11.270122203000351	24.32501300930069
2	16.00487498637736	21.460070699582033	37.93571341956656	24.599340894474043
3	18.96772708085498	26.981695383552704	28.14336697494148	25.907210560650828
4	11.629352851958515	15.741343420139636	36.617071837792366	36.01223189010948
5	13.686830456191856	38.12413344122294	32.65308629274831	15.535949809836891
6	32.03120921104761	36.41345908743309	17.878191327635292	13.677140373884015
7	28.702223246169794	31.70740698076259	21.650654686480053	17.939715086587565
8	28.324356543941203	31.98492022281692	20.454636232636595	19.23608700060528
9	28.058019287526097	14.209686166723257	19.92020120235924	37.8120933433914
10	17.03645762899399	25.961231113460613	30.24412657823238	26.75818467931301
11	36.93366137164477	21.749185923038034	22.7963063418862	18.520846363431
12	25.634992115431317	24.596319305051352	28.658639673674145	21.110048905843186
13	29.425279230877987	19.828926905132192	26.382630825121865	24.363163038867956
14	23.615943543528775	21.40169910372893	25.576767030994503	29.405590321747788
15	24.38123014842486	28.423383484411474	24.07835676978089	23.11702959738278
16	24.503773274673804	26.192104320941173	26.031215761239533	23.272906643145486
17	22.315572384880657	26.596856385888774	26.752838411841722	24.334732817388847
18	23.84001327894703	23.929615433087324	29.941873868446212	22.288497419519437
19	24.80189224413515	25.68592312988737	26.852438154723846	22.659746471253627
20	25.070113457019875	23.783406661124182	27.286082882174046	23.860396999681896
21	26.90898472320413	24.12704023606799	25.00491808317708	23.959056957550796
22	25.52022566041796	25.277656083070088	25.99783255320621	23.20428570330574
23	22.539616667435176	24.96165137332657	27.52048077957609	24.978251179662163
24	23.69256685100196	26.297750166762473	27.03150504283567	22.978177939399902
25	23.29477254668714	25.717693238107092	27.06171109072159	23.92582312448418
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	547.0
1	547.0
2	889.5
3	1232.0
4	1232.0
5	1232.0
6	3115.5
7	4999.0
8	4999.0
9	4999.0
10	4780.5
11	4562.0
12	4562.0
13	4562.0
14	3684.5
15	2807.0
16	2807.0
17	2807.0
18	4374.5
19	5942.0
20	5942.0
21	5942.0
22	9842.0
23	13742.0
24	13742.0
25	13742.0
26	20943.5
27	28145.0
28	28145.0
29	28145.0
30	33168.5
31	38192.0
32	38192.0
33	38192.0
34	42987.0
35	47782.0
36	47782.0
37	47782.0
38	49697.0
39	51612.0
40	51612.0
41	51612.0
42	57977.0
43	64342.0
44	64342.0
45	64342.0
46	77447.0
47	90552.0
48	90552.0
49	90552.0
50	85298.5
51	80045.0
52	80045.0
53	80045.0
54	66986.5
55	53928.0
56	53928.0
57	53928.0
58	50485.0
59	47042.0
60	47042.0
61	47042.0
62	44605.0
63	42168.0
64	42168.0
65	42168.0
66	36552.5
67	30937.0
68	30937.0
69	30937.0
70	23702.5
71	16468.0
72	16468.0
73	16468.0
74	13913.0
75	11358.0
76	11358.0
77	11358.0
78	10033.0
79	8708.0
80	8708.0
81	8708.0
82	6091.5
83	3475.0
84	3475.0
85	3475.0
86	2603.0
87	1731.0
88	1731.0
89	1731.0
90	1228.5
91	726.0
92	726.0
93	726.0
94	421.0
95	116.0
96	116.0
97	116.0
98	233.0
99	350.0
100	350.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00782799290261977
2	0.0029163110813681488
3	0.005065171878165732
4	0.014735045463754858
5	0.03453526280567545
6	0.0445121165050928
7	0.06646119464381096
8	0.08748933244104447
9	0.09531732534366424
10	0.11972224439300821
11	0.1183408338807812
12	0.1335363495152784
13	0.12156412507597758
14	0.12463392621425984
15	0.1137361321733578
16	0.11465707251484247
17	0.12156412507597758
18	0.1310805086046526
19	0.13077352849082435
20	0.11926177422226587
21	0.1301595682631679
22	0.14934582537743207
23	0.13783407110887358
24	0.13537823019824777
25	0.1300060782062538
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	651508.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.02147748763986
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15843035487707	29.30562047339989
2	12.585281403436083	9.31851424305843
3	3.34878806714128	3.719312461156436
4	1.3239405286512664	1.9605693790574747
5	0.6587882724505231	1.2194657598824097
6	0.3957573468344983	0.8790913023840876
7	0.28464197411418324	0.7376506505693922
8	0.21624282725866187	0.6404503168976117
9	0.15081455321753698	0.5025039828076358
>10	1.3204569955212668	10.375893457611978
>50	0.24352134211487417	6.41625011595541
>100	0.2742731839424376	21.23770326661342
>500	0.028258449254675386	6.960719823494545
>1k	0.010804701185611177	6.726254767111254
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4255	0.6531001921695512	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3546	0.544275741817445	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2715	0.4167255045218171	No Hit
GTACATGGAAGCAGTGGTATCAACG	2684	0.4119673127574796	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2632	0.4039858297979457	No Hit
TCCATGTACTCTGCGTTGATACCAC	2232	0.34258980703230046	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1819	0.27919841352677177	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1535	0.23560723736316364	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1492	0.22900716491585674	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1409	0.21626749019198538	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1400	0.21488607967975834	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1383	0.21227674871221844	No Hit
GAACTACGACGGTATCTGATCGTCT	1355	0.20797902711862326	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1355	0.20797902711862326	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1328	0.2038347955819422	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1324	0.20322083535428576	No Hit
GTACATGGGGTGGTATCAACGCAAA	1295	0.19876962370377646	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1282	0.196774252963893	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1207	0.1852624986953345	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1170	0.17958336658951232	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1101	0.16899255266243854	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1066	0.16362040067044456	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1053	0.1616250299305611	No Hit
GAATAATGGAATAGGACCGCGGTTC	1038	0.1593226790768494	No Hit
CATGTACTCTGCGTTGATACCACTG	1028	0.15778777850770825	No Hit
CTTTAATATACGCTATTGGAGCTGG	1016	0.15594589782473892	No Hit
TCGTAGTTCCGACCATAAACGATGC	966	0.14827139497903327	No Hit
GCTTTGAACACTCTAATTTTTTCAA	937	0.14382018332852398	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	928	0.14243877281629697	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	922	0.14151783247481228	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	906	0.13906199156418647	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	867	0.13307587934453607	No Hit
CCATTATTCCTAGCTGCGGTATCCA	863	0.1324619191168796	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	850	0.13046654837699614	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	845	0.12969909809242558	No Hit
GTACATGGGGAATAATTGCAATCCC	836	0.12831768758019857	No Hit
GTCCTACAGTGGACATTTCTAAATT	821	0.12601533672648688	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	821	0.12601533672648688	No Hit
GTACATGGGTGGTATCAACGCAAAA	816	0.1252478864419163	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	815	0.12509439638500217	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	813	0.12478741627117394	No Hit
GAATAATTGCAATCCCCGATCCCCA	808	0.12401996598660339	No Hit
CTTTAAATCCTTTAACGAGGATCCA	778	0.11941526427918	No Hit
CTGTAGGACGTGGAATATGGCAAGA	765	0.11741989353929654	No Hit
CTTTAGGACGTGAAATATGGCGAGG	739	0.11342915205952958	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	738	0.11327566200261548	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	734	0.11266170177495902	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	728	0.11174076143347433	No Hit
CTCCAATAGCGTATATTAAAGTTGC	715	0.10974539069359088	No Hit
CATCTAAGGGCATCACAGACCTGTT	691	0.10606162932765215	No Hit
ACCATACTCCCCCCGGAACCCAAAG	690	0.10590813927073804	No Hit
GCTTCCATGTACTCTGCGTTGATAC	688	0.1056011591569098	No Hit
GGTCGGGAGTGGGTAATTTGCGCGC	687	0.1054476690999957	No Hit
GCCATGCACCACCACCCACGGAATC	670	0.10283833813245577	No Hit
GTATCAACGCAGAGTACATGGGGTG	667	0.10237786796171344	No Hit
GGATTGACAGATTGATAGCTCTTTC	665	0.1020708878478852	No Hit
GCGTTGATACCACTGCTTCCATGTA	657	0.10084296739257231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.534900569141131E-4
7	0.0	0.0	0.0	0.0	1.534900569141131E-4
8	0.0	0.0	0.0	0.0	1.534900569141131E-4
9	0.0	0.0	0.0	1.534900569141131E-4	1.534900569141131E-4
10	0.0	0.0	0.0	3.069801138282262E-4	1.534900569141131E-4
11	0.0	0.0	0.0	3.069801138282262E-4	1.534900569141131E-4
12	0.0	0.0	0.0	3.069801138282262E-4	4.604701707423393E-4
13	0.0	0.0	0.0	3.069801138282262E-4	6.139602276564524E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATAAC	80	2.1827873E-11	17.812933	10
TAACGAA	90	7.2759576E-12	16.890596	13
ATGTACG	35	0.0021669127	16.287361	17
TCCGTTG	35	0.0021680256	16.28611	12
TACGCCT	35	0.0021691388	16.284859	5
GTACAGG	35	0.0021735965	16.279854	1
GTTTAAA	60	1.4691177E-6	15.827637	1
ACGAACG	95	3.0377123E-10	15.001516	15
TTATACT	45	6.757554E-4	14.777	4
ATCGCCA	205	0.0	14.367307	16
GATAACG	100	6.566552E-10	14.250345	11
CCGATAA	100	6.566552E-10	14.250345	9
ATAACGA	100	6.566552E-10	14.250345	12
CAATTAG	60	2.5724534E-5	14.244873	1
GGATAAA	40	0.005287351	14.244873	1
CCATTAA	40	0.005287351	14.244873	1
CATCGCC	210	0.0	14.025228	15
TCGCCAG	205	0.0	13.903846	17
CGAACGA	105	1.3624231E-9	13.572801	16
AACCGCG	140	0.0	13.571758	7
>>END_MODULE