##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062634_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651508
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09621677707718	32.0	32.0	32.0	32.0	32.0
2	31.385768094942808	32.0	32.0	32.0	32.0	32.0
3	31.490142868544975	32.0	32.0	32.0	32.0	32.0
4	31.6030102469962	32.0	32.0	32.0	32.0	32.0
5	31.5334746465124	32.0	32.0	32.0	32.0	32.0
6	35.111911135396646	36.0	36.0	36.0	36.0	36.0
7	35.11225341822357	36.0	36.0	36.0	36.0	36.0
8	35.06776739502815	36.0	36.0	36.0	36.0	36.0
9	35.14023772540015	36.0	36.0	36.0	36.0	36.0
10	35.043924249587114	36.0	36.0	36.0	36.0	36.0
11	35.16743002388305	36.0	36.0	36.0	36.0	36.0
12	35.0645717320432	36.0	36.0	36.0	36.0	36.0
13	35.10872928651682	36.0	36.0	36.0	36.0	36.0
14	35.06495699208605	36.0	36.0	36.0	36.0	36.0
15	35.043080054274085	36.0	36.0	36.0	36.0	36.0
16	35.04306777506953	36.0	36.0	36.0	36.0	36.0
17	35.01896830123344	36.0	36.0	36.0	36.0	36.0
18	35.028368032318866	36.0	36.0	36.0	36.0	36.0
19	34.989215788601214	36.0	36.0	36.0	36.0	36.0
20	34.94484027824677	36.0	36.0	36.0	36.0	36.0
21	34.93013439589384	36.0	36.0	36.0	32.0	36.0
22	34.926008583163984	36.0	36.0	36.0	32.0	36.0
23	34.89120624765928	36.0	36.0	36.0	32.0	36.0
24	34.877932427537345	36.0	36.0	36.0	32.0	36.0
25	34.846384081239215	36.0	36.0	36.0	32.0	36.0
26	34.81101076272279	36.0	36.0	36.0	32.0	36.0
27	34.79418057798216	36.0	36.0	36.0	32.0	36.0
28	34.738867366172016	36.0	36.0	36.0	32.0	36.0
29	34.705879283140035	36.0	36.0	36.0	32.0	36.0
30	34.66494348496104	36.0	36.0	36.0	32.0	36.0
31	34.66729188283183	36.0	36.0	36.0	32.0	36.0
32	34.60775615955598	36.0	36.0	36.0	32.0	36.0
33	34.546381625398304	36.0	36.0	36.0	32.0	36.0
34	34.506253184918684	36.0	36.0	36.0	32.0	36.0
35	34.43327019775659	36.0	36.0	36.0	32.0	36.0
36	34.38993995468974	36.0	36.0	36.0	32.0	36.0
37	34.34676934128207	36.0	36.0	36.0	32.0	36.0
38	34.27835421821374	36.0	36.0	36.0	32.0	36.0
39	34.22474474603535	36.0	36.0	36.0	32.0	36.0
40	34.17544680955568	36.0	36.0	36.0	32.0	36.0
41	34.12164700970671	36.0	36.0	36.0	32.0	36.0
42	34.048280297402336	36.0	36.0	36.0	32.0	36.0
43	34.04446606948802	36.0	36.0	36.0	32.0	36.0
44	33.9552745323158	36.0	36.0	36.0	32.0	36.0
45	33.82940040644167	36.0	36.0	36.0	32.0	36.0
46	33.80551121398356	36.0	36.0	36.0	32.0	36.0
47	33.72503791204406	36.0	36.0	36.0	27.0	36.0
48	33.65954984436108	36.0	36.0	36.0	27.0	36.0
49	33.626518784113166	36.0	36.0	36.0	21.0	36.0
50	33.08094451641423	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	4.0
20	12.0
21	38.0
22	97.0
23	259.0
24	622.0
25	1542.0
26	3162.0
27	5934.0
28	9622.0
29	14285.0
30	19255.0
31	25891.0
32	37037.0
33	56026.0
34	111826.0
35	365892.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.42117867395343	19.08598271441949	11.856588016763636	24.63625059486345
2	15.443758826057591	21.93651378399951	37.650273224043715	24.969454165899183
3	18.382332626253632	27.2439547755508	28.64394188234644	25.729770715849128
4	11.443297703174789	16.130116591047233	36.91635405858408	35.5102316471939
5	13.382337592170778	38.18924710057283	32.710879989194304	15.717535318062096
6	32.053015709779665	36.6809157258962	17.457118517893186	13.808950046430956
7	28.32459463275969	31.67942680673146	21.988678573402016	18.007299987106833
8	28.215156222179928	31.995923304088357	20.53175095317325	19.25716952055846
9	28.05046139111108	13.747797417683284	19.98532635055901	38.21641484064662
10	17.006084345856078	25.760389741952515	30.41773853889745	26.815787373293958
11	37.04467174616428	21.40265353610393	23.017368934840402	18.535305782891385
12	25.539671040110022	24.564395218477745	28.8880566316914	21.007877109720834
13	29.547910386365174	19.656704138705894	26.60642693566311	24.188958539265826
14	23.709609091523053	21.37272297500568	25.721403267496328	29.196264665974937
15	24.420114564978483	28.23250059861122	24.348741688513417	22.99864314789688
16	24.46155688034529	25.89039582015877	26.26199524794784	23.386052051548102
17	22.24638837896081	26.310805086046525	26.95561681514271	24.487189719849948
18	23.826875495005435	23.309000042977214	30.48189738268755	22.3822270793298
19	24.96147399571456	25.162545970272042	27.404114761445754	22.471865272567644
20	25.28625895614482	23.19986861251128	27.842328873935546	23.671543557408352
21	27.031440903258286	23.58236583434125	25.59891820207887	23.78727506032159
22	25.48518206990551	24.883347556745274	26.566673010922354	23.064797362426862
23	22.38253405944363	24.42195644566145	28.266268411132327	24.929241083762594
24	23.835470938192625	25.64343031858396	27.610865868109062	22.91023287511435
25	23.56394702751156	24.80000245584091	27.780932851169904	23.85511766547763
26	22.01047416148382	26.509728199807213	28.8598144612192	22.61998317748976
27	24.21965655064865	25.411353352529826	27.41900329696642	22.949986799855104
28	22.307016951441884	25.02563283950466	28.616532721010334	24.050817488043126
29	22.452985995567207	25.217648900704216	28.562197240862737	23.767167862865843
30	22.549224261252355	25.404292809911773	29.063342276687315	22.983140652148553
31	23.37454029727954	25.327394291397802	26.983245025387255	24.3148203859354
32	22.81169225857549	25.586025037298082	27.251392154816212	24.35089054931022
33	22.014464902963585	24.972985749983113	28.482228921210485	24.530320425842813
34	23.41705704304475	25.419181345432445	28.764804115989367	22.398957495533438
35	25.025479349447743	25.256942355274226	27.82790080858562	21.889677486692413
36	22.28706324404305	26.371740638641427	27.667964169281113	23.673231948034406
37	24.306378432805122	26.236976368670838	26.591384910085523	22.865260288438517
38	22.718523794028624	25.842199942287742	27.606414656458554	23.832861607225084
39	23.777298206622174	25.208439497289366	26.947328352069356	24.066933944019105
40	24.54766480227411	25.69070525611351	27.66827114939494	22.09335879221744
41	22.323747367645524	26.142119513497914	28.66672396962125	22.867409149235314
42	24.78649533083247	27.301122933256384	27.05246904105552	20.859912694855627
43	22.88260466486981	25.403832339741033	27.550390785684904	24.163172209704257
44	22.0777028064122	26.498676915709403	27.204270707343582	24.21934957053482
45	22.58115019309049	27.04126426690079	26.791382454244616	23.586203085764105
46	22.30935354493505	26.030879177046444	27.095487845871187	24.564279432147316
47	23.522197732030918	25.13031305832008	27.57494919479116	23.77254001485784
48	23.925876581715038	27.565279321205573	25.27582163227466	23.23302246480473
49	22.3441615452151	27.077180940218692	26.566673010922354	24.011984503643856
50	22.03644780485858	28.589101882251455	25.325284302394298	24.049166010495664
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	162.0
1	146.5
2	131.0
3	385.5
4	640.0
5	664.5
6	689.0
7	534.5
8	380.0
9	549.5
10	719.0
11	1076.5
12	1434.0
13	2336.5
14	3239.0
15	4201.5
16	5164.0
17	5647.5
18	6131.0
19	6354.0
20	6577.0
21	7054.5
22	7532.0
23	7356.0
24	7180.0
25	7212.0
26	7244.0
27	8429.5
28	9615.0
29	10927.0
30	12239.0
31	13465.0
32	14691.0
33	17162.5
34	19634.0
35	22734.0
36	25834.0
37	29443.0
38	33052.0
39	32970.0
40	32888.0
41	33978.0
42	35068.0
43	33134.0
44	31200.0
45	35483.0
46	39766.0
47	42696.0
48	45626.0
49	45384.5
50	45143.0
51	39065.5
52	32988.0
53	31357.5
54	29727.0
55	32514.5
56	35302.0
57	36094.5
58	36887.0
59	33202.0
60	29517.0
61	26224.5
62	22932.0
63	20111.0
64	17290.0
65	14952.0
66	12614.0
67	11852.0
68	11090.0
69	10873.0
70	10656.0
71	8225.5
72	5795.0
73	5665.5
74	5536.0
75	4093.5
76	2651.0
77	2317.0
78	1983.0
79	1773.5
80	1564.0
81	1310.5
82	1057.0
83	956.5
84	856.0
85	750.5
86	645.0
87	471.5
88	298.0
89	199.5
90	101.0
91	65.0
92	29.0
93	15.5
94	2.0
95	2.5
96	3.0
97	3.5
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.015042025577583084
2	0.004297721593595167
3	3.0698011382822624E-4
4	0.0
5	0.0
6	4.604701707423393E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	1.5349005691411312E-4
47	0.0
48	0.0
49	0.0
50	1.5349005691411312E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	651508.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.466876091723705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.28536222036722	34.89753891611858
2	11.849334350052574	10.301070959262814
3	3.3027076480062028	4.306751523092216
4	1.2478970380989955	2.169687437211122
5	0.6830013899055539	1.484396839274989
6	0.4022918942911746	1.0491823153115578
7	0.2784361747309524	0.8471925494538686
8	0.20435826602757814	0.71062523421922
9	0.17178947733450275	0.6720436732644729
>10	1.1406951573782595	10.179160486448666
>50	0.19716869341204254	5.988405615920045
>100	0.21102379024687304	18.532401283708193
>500	0.01953923983767343	5.471716456441521
>1k	0.006394660310511305	3.38982671027274
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1513	0.2322304561110531	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1483	0.22762575440362973	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1441	0.22117917201323697	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1410	0.21642098024889947	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1343	0.20613714643565392	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1315	0.20183942484205872	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1276	0.19585331262240835	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1256	0.19278351148412606	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1255	0.19263002142721194	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1140	0.17497866488208894	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1127	0.17298329414220545	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	1097	0.16837859243478207	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1088	0.16699718192255505	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1087	0.16684369186564096	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	1085	0.16653671175181273	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	1014	0.1556389177109107	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	1012	0.15533193759708247	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	1010	0.15502495748325423	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	988	0.15164817623114374	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAAT	939	0.1441271634423522	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	894	0.1372201108812171	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	875	0.13430379979984897	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	847	0.1300060782062538	No Hit
GAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTAC	815	0.12509439638500217	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	809	0.1241734560435175	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA	801	0.1229455355882046	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT	779	0.11956875433609411	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	773	0.11864781399460944	No Hit
CCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACAC	762	0.11695942336855418	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	748	0.1148105625717566	No Hit
CTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGA	746	0.11450358245792837	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT	732	0.11235472166113081	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	724	0.11112680120581787	No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC	718	0.1102058608643332	No Hit
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC	684	0.10498719892925337	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT	680	0.10437323870159691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.534900569141131E-4	0.0
11	0.0	0.0	0.0	1.534900569141131E-4	0.0
12	0.0	0.0	0.0	1.534900569141131E-4	0.0
13	0.0	0.0	0.0	1.534900569141131E-4	0.0
14	0.0	0.0	0.0	1.534900569141131E-4	0.0
15	0.0	0.0	0.0	1.534900569141131E-4	0.0
16	0.0	0.0	0.0	1.534900569141131E-4	0.0
17	0.0	0.0	0.0	4.604701707423393E-4	0.0
18	0.0	0.0	0.0	7.674502845705655E-4	1.534900569141131E-4
19	0.0	0.0	0.0	0.0012279204553129047	1.534900569141131E-4
20	0.0	0.0	0.0	0.0016883906260552442	1.534900569141131E-4
21	0.0	0.0	0.0	0.003069801138282262	1.534900569141131E-4
22	0.0	0.0	0.0	0.005679132105822185	1.534900569141131E-4
23	0.0	0.0	0.0	0.011665244325472597	1.534900569141131E-4
24	0.0	0.0	0.0	0.020107197455748817	1.534900569141131E-4
25	0.0	0.0	0.0	0.026860759959969793	1.534900569141131E-4
26	0.0	0.0	0.0	0.033921302578018994	1.534900569141131E-4
27	0.0	0.0	0.0	0.042056275594466994	1.534900569141131E-4
28	0.0	0.0	0.0	0.05894018185501943	1.534900569141131E-4
29	0.0	0.0	0.0	0.08917772306709972	1.534900569141131E-4
30	0.0	0.0	0.0	0.14550857395457922	1.534900569141131E-4
31	0.0	0.0	0.0	0.23929099872910234	1.534900569141131E-4
32	0.0	0.0	0.0	0.3550225016423436	1.534900569141131E-4
33	0.0	0.0	0.0	0.4656888326774192	1.534900569141131E-4
34	0.0	0.0	0.0	0.5820342958183169	1.534900569141131E-4
35	0.0	0.0	0.0	0.720482327154847	1.534900569141131E-4
36	0.0	0.0	0.0	0.921400811655421	1.534900569141131E-4
37	0.0	0.0	0.0	1.1809524978971861	1.534900569141131E-4
38	0.0	0.0	0.0	1.5028211472460813	1.534900569141131E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACGGGT	25	0.0023521	35.19969	7
TAGTACG	40	7.0226815E-4	27.503983	2
TACGCCT	50	8.33613E-5	26.399769	5
GCACTAG	50	0.0025770057	22.003185	1
CGGGGCG	50	0.0025793037	21.999807	14
TAGGACG	535	0.0	21.38299	4
GTCCTAA	270	0.0	21.188251	1
GGACGTG	545	0.0	20.990643	6
CGTGCAA	105	7.73307E-8	20.9522	11
TGTACTG	85	6.1067676E-6	20.705702	5
GATATAC	160	1.8189894E-12	20.627987	1
GACGTGA	235	0.0	20.595564	7
TATACTG	65	4.929097E-4	20.307514	5
AGGACGT	575	0.0	20.278084	5
TATAGGA	55	0.004477678	20.002895	2
TTAGGAC	330	0.0	20.00136	3
TGACCGT	110	1.2635064E-7	19.999826	7
ACTGTCC	110	1.2635064E-7	19.999826	8
CGTTTTG	55	0.004481658	19.999826	30
CCTTGTA	110	1.2635064E-7	19.999826	8
>>END_MODULE