FastQCFastQC Report
Thu 2 Feb 2017
SRR4062633_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062633_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG170.8525576730190572No Hit
TCCATGTACTCTGCGTTGATACCAC120.6018054162487462No Hit
GTATCAACGCAGAGTACTTTTTTTT110.551654964894684No Hit
TATCAACGCAGAGTACTTTTTTTTT100.5015045135406219No Hit
GCTTCCATGTACTCTGCGTTGATAC70.3510531594784353No Hit
GAGTACATGGAAGCAGTGGTATCAA70.3510531594784353No Hit
CATGTACTCTGCGTTGATACCACTG60.3009027081243731No Hit
ATACAGGACTCTTTCGAGGCCCTGT50.25075225677031093No Hit
GGCGGTGACTCGCCTCGCGGCGGAC50.25075225677031093No Hit
GTATCAACGCAGAGTACATGGAAGC50.25075225677031093No Hit
CATGGAAGCAGTGGTATCAACGCAG50.25075225677031093No Hit
GGTATCAACGCAGAGTACTTTTTTT50.25075225677031093No Hit
ACTCTGCGTTGATACCACTGCTTCC40.20060180541624875No Hit
GTACATGGGAAGCAGTGGTATCAAC40.20060180541624875No Hit
GAGTACTTTTTTTTTTTTTTTTTTT40.20060180541624875No Hit
AAAGTACTCTGCGTTGATACCACTG40.20060180541624875No Hit
GTCCTATTCCATTATTCCTAGCTGC40.20060180541624875No Hit
ACGCAGAGTACTTTTTTTTTTTTTT40.20060180541624875No Hit
GTATCTGATCGTCTTCGAACCTCCG30.15045135406218654No Hit
GAATAACGCCGCCGCATCGCCAGTC30.15045135406218654No Hit
GTGCATGGCCGTTCTTAGTTGGTGG30.15045135406218654No Hit
GAACGAGACTCTGGCATGCTAACTA30.15045135406218654No Hit
CTGAAGGACCTGGAATATGGCGAGA30.15045135406218654No Hit
CTATTGGAGCTGGAATTACCGCGGC30.15045135406218654No Hit
AAGCAGTGGTATCAACGCAGAGTAC30.15045135406218654No Hit
CCCATGTACTCTGCGTTGATACCAC30.15045135406218654No Hit
GCTTCCCATGTACTCTGCGTTGATA30.15045135406218654No Hit
CTGTAGACCAGGCTGGCCTCGAACT30.15045135406218654No Hit
TCGTAGTTCCGACCATAAACGATGC30.15045135406218654No Hit
CTTTAGGACGTGAAATATGGCGAGG30.15045135406218654No Hit
GTACATGGGTACCTGGTTGATCCTG30.15045135406218654No Hit
GTATCAACGCAGAGTACATGGGAAT30.15045135406218654No Hit
TAACAATACAGGACTCTTTCGAGGC20.10030090270812438No Hit
ATCAGACATTAATTGAATTTATTAA20.10030090270812438No Hit
GGTTATCTAGAGTCACCAAAGCCGC20.10030090270812438No Hit
CCATAGACGCTTTCATCACTGTAGG20.10030090270812438No Hit
CCCTGATGGAGTCTGTGTTACTCAG20.10030090270812438No Hit
GCTCCACTCCTGGTGGTGCCCTTCC20.10030090270812438No Hit
GTAGTCGCCGTGCCTACCATGGTGA20.10030090270812438No Hit
GACCAGAGCGAAAGCATTTGCCAAG20.10030090270812438No Hit
GTATCAACGCAGAGTACATGGGCGA20.10030090270812438No Hit
AAATAGAACCGCGGTCCTATTCCAT20.10030090270812438No Hit
CTCCAATAGCGTATATTAAAGTTGC20.10030090270812438No Hit
GATACCACTGCTTCCCATGTACTCT20.10030090270812438No Hit
GTTCTATTTTGTTGGTTTTCGGAAC20.10030090270812438No Hit
GTGGTAGCCGTTTCTCAGGCTCCCT20.10030090270812438No Hit
CAGTGGTATCAACGCAGAGTACATG20.10030090270812438No Hit
ATTCCTAGCTGCGGTATCCAGGCGG20.10030090270812438No Hit
CTTAGTTGGTGGAGCGATTTGTCTG20.10030090270812438No Hit
CCTGGATACCGCAGCTAGGAATAAT20.10030090270812438No Hit
GATTGATAGCTCTTTCTCGATTCCG20.10030090270812438No Hit
CCATCCTACAGCCACCAGGTCTAAT20.10030090270812438No Hit
AATAAGATATGTTGATTTTACTTTG20.10030090270812438No Hit
CATACACACAGTCCGAGTTCGAGGA20.10030090270812438No Hit
ATACCACTGCTTCCATGTACTCTCA20.10030090270812438No Hit
GAACTACGACGGTATCTGATCGTCT20.10030090270812438No Hit
CCACTGCTTCCATGTACTCTGCGTT20.10030090270812438No Hit
GTACAGTGAAACTGCGAATGGCTCA20.10030090270812438No Hit
CCTCGAAAGAGTCCTGTATTGTTAT20.10030090270812438No Hit
GGTATCAACGCAGAGTACATGGGGG20.10030090270812438No Hit
GGTATCAACGCAGAGTACATGGGAA20.10030090270812438No Hit
AAAGTGGACTCATTCCAATTACAGG20.10030090270812438No Hit
GTCCTGTATTGTTATTTTTCGTCAC20.10030090270812438No Hit
ATCAACGCAGAGTACTTTTTTTTTT20.10030090270812438No Hit
GTGTAGAAGCTATTAATGGTTCGTT20.10030090270812438No Hit
GGATTATCCGCGGCAACGCAACTGA20.10030090270812438No Hit
CTCCAGCGTGGCCCGAAATCCTTCT20.10030090270812438No Hit
GTCCTACAGTGTGCATTTCTCATTT20.10030090270812438No Hit
GTCCTAAAGTGTGTATTTCTCATTT20.10030090270812438No Hit
ATCCTAGCCCTAGCCCTACACAAAT20.10030090270812438No Hit
ACTCACGCCACCCACCGGAGAATGG20.10030090270812438No Hit
CAAATTACCCACTCCCGACCCGGGG20.10030090270812438No Hit
TTCCAGAAGAGCTGTCCCTCTTTTG20.10030090270812438No Hit
CTGTAGGACCTGGAATATGGCGAGA20.10030090270812438No Hit
CCCAAGATCCAACTACGAGCTTTTT20.10030090270812438No Hit
GTATCAACGCAGAGTACATGGGAAG20.10030090270812438No Hit
GCGTTGATACCACTGCTTCCATGTA20.10030090270812438No Hit
CGCTTAAACTCATCCAACACAAAGG20.10030090270812438No Hit
GGAGTGGAGCCTGCGGCTTAATTTG20.10030090270812438No Hit
GTACATGGAAGCAGTGGTATCAACT20.10030090270812438No Hit
GGCTATGACGAGCATGAAGGGCCAG20.10030090270812438No Hit
ACCTCGGAGTTGGGCGTCCGAGCGT20.10030090270812438No Hit
GGTTAATTCCGATAACGAACGAGAC20.10030090270812438No Hit
CCATGTACTCTGCGTTGATACCACT20.10030090270812438No Hit
CTGTCAATCCTGTCCGTGTCCGGGC20.10030090270812438No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)