##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062633_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1994
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.312938816449346	32.0	32.0	32.0	14.0	32.0
2	27.957372116349045	32.0	32.0	32.0	14.0	32.0
3	28.54112337011033	32.0	32.0	32.0	14.0	32.0
4	27.647943831494484	32.0	21.0	32.0	14.0	32.0
5	27.98545636910732	32.0	21.0	32.0	14.0	32.0
6	31.39518555667001	36.0	32.0	36.0	14.0	36.0
7	31.446339017051155	36.0	32.0	36.0	14.0	36.0
8	31.065697091273822	36.0	32.0	36.0	14.0	36.0
9	31.14493480441324	36.0	32.0	36.0	14.0	36.0
10	30.256268806419257	36.0	21.0	36.0	14.0	36.0
11	31.74122367101304	36.0	32.0	36.0	14.0	36.0
12	30.948345035105316	36.0	32.0	36.0	14.0	36.0
13	31.23219658976931	36.0	32.0	36.0	14.0	36.0
14	30.72768304914744	36.0	32.0	36.0	14.0	36.0
15	30.58324974924774	36.0	27.0	36.0	14.0	36.0
16	30.610832497492478	36.0	27.0	36.0	14.0	36.0
17	30.71765295887663	36.0	32.0	36.0	14.0	36.0
18	30.464894684052158	36.0	27.0	36.0	14.0	36.0
19	30.626379137412236	36.0	32.0	36.0	14.0	36.0
20	30.43530591775326	36.0	27.0	36.0	14.0	36.0
21	30.440822467402207	36.0	27.0	36.0	14.0	36.0
22	30.134403209628886	36.0	27.0	36.0	14.0	36.0
23	30.486960882647942	36.0	32.0	36.0	14.0	36.0
24	30.50100300902708	36.0	27.0	36.0	14.0	36.0
25	29.617352056168507	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	0.0
9	0.0
10	1.0
11	0.0
12	0.0
13	1.0
14	5.0
15	5.0
16	12.0
17	21.0
18	30.0
19	41.0
20	35.0
21	51.0
22	46.0
23	51.0
24	61.0
25	61.0
26	62.0
27	69.0
28	81.0
29	102.0
30	108.0
31	126.0
32	162.0
33	196.0
34	342.0
35	324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.427281845536605	18.756268806419257	12.337011033099298	26.479438314944836
2	14.894684052156471	21.213640922768302	39.06720160481444	24.82447342026078
3	19.408224674022065	24.32296890672016	29.13741223671013	27.13139418254764
4	11.183550651955867	16.599799398194584	36.158475426278834	36.05817452357071
5	14.952333166081285	36.22679377822378	32.162568991470145	16.658304064224787
6	31.86151530356247	36.22679377822378	17.711991971901657	14.199698946312092
7	29.001505268439537	30.65730055193176	20.17059708981435	20.17059708981435
8	25.89061716006021	35.12293025589563	19.36778725539388	19.618665328650277
9	26.292022077270445	15.203211239337683	17.561465127947816	40.94330155544406
10	14.414866901054745	26.971371170266195	33.350075339025615	25.26368658965344
11	37.537688442211056	21.55778894472362	21.35678391959799	19.547738693467338
12	24.472361809045225	23.919597989949747	28.492462311557787	23.115577889447238
13	28.34170854271357	21.155778894472363	25.125628140703515	25.376884422110553
14	22.91457286432161	19.849246231155778	26.633165829145728	30.60301507537688
15	25.36413862380713	26.92114515318935	24.962330487192368	22.75238573581115
16	25.326633165829143	25.628140703517587	26.13065326633166	22.91457286432161
17	22.373051784816493	25.540472599296127	28.305681246857716	23.78079436902966
18	23.26633165829146	24.22110552763819	28.190954773869347	24.321608040201003
19	25.590749120160883	26.44544997486174	26.495726495726498	21.468074409250878
20	23.316582914572866	26.231155778894472	27.18592964824121	23.26633165829146
21	26.080402010050253	24.974874371859297	23.417085427135678	25.52763819095477
22	22.060301507537687	26.28140703517588	25.678391959798997	25.979899497487434
23	22.512562814070353	26.331658291457288	27.1356783919598	24.020100502512562
24	24.032176973353444	25.540472599296127	26.294620412267474	24.132730015082956
25	22.27249874308698	24.484665661136248	28.657616892911008	24.58521870286576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	1.0
5	1.0
6	2.0
7	3.0
8	3.0
9	3.0
10	5.0
11	7.0
12	7.0
13	7.0
14	9.5
15	12.0
16	12.0
17	12.0
18	14.5
19	17.0
20	17.0
21	17.0
22	23.0
23	29.0
24	29.0
25	29.0
26	52.5
27	76.0
28	76.0
29	76.0
30	84.0
31	92.0
32	92.0
33	92.0
34	118.0
35	144.0
36	144.0
37	144.0
38	156.5
39	169.0
40	169.0
41	169.0
42	203.0
43	237.0
44	237.0
45	237.0
46	274.5
47	312.0
48	312.0
49	312.0
50	287.5
51	263.0
52	263.0
53	263.0
54	232.0
55	201.0
56	201.0
57	201.0
58	181.0
59	161.0
60	161.0
61	161.0
62	134.5
63	108.0
64	108.0
65	108.0
66	88.0
67	68.0
68	68.0
69	68.0
70	56.0
71	44.0
72	44.0
73	44.0
74	31.5
75	19.0
76	19.0
77	19.0
78	19.5
79	20.0
80	20.0
81	20.0
82	13.5
83	7.0
84	7.0
85	7.0
86	5.0
87	3.0
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.05015045135406219
6	0.05015045135406219
7	0.05015045135406219
8	0.05015045135406219
9	0.05015045135406219
10	0.15045135406218654
11	0.20060180541624875
12	0.20060180541624875
13	0.20060180541624875
14	0.20060180541624875
15	0.15045135406218654
16	0.20060180541624875
17	0.25075225677031093
18	0.20060180541624875
19	0.25075225677031093
20	0.20060180541624875
21	0.20060180541624875
22	0.20060180541624875
23	0.20060180541624875
24	0.25075225677031093
25	0.25075225677031093
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1994.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.87261785356068
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.30905077262693	86.6098294884654
2	2.924944812362031	5.315947843530592
3	0.772626931567329	2.106318956870612
4	0.33112582781456956	1.2036108324974923
5	0.27593818984547464	1.2537612838515546
6	0.05518763796909492	0.3009027081243731
7	0.11037527593818984	0.7021063189568706
8	0.0	0.0
9	0.0	0.0
>10	0.22075055187637968	2.507522567703109
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	17	0.8525576730190572	No Hit
TCCATGTACTCTGCGTTGATACCAC	12	0.6018054162487462	No Hit
GTATCAACGCAGAGTACTTTTTTTT	11	0.551654964894684	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10	0.5015045135406219	No Hit
GCTTCCATGTACTCTGCGTTGATAC	7	0.3510531594784353	No Hit
GAGTACATGGAAGCAGTGGTATCAA	7	0.3510531594784353	No Hit
CATGTACTCTGCGTTGATACCACTG	6	0.3009027081243731	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	5	0.25075225677031093	No Hit
GGCGGTGACTCGCCTCGCGGCGGAC	5	0.25075225677031093	No Hit
GTATCAACGCAGAGTACATGGAAGC	5	0.25075225677031093	No Hit
CATGGAAGCAGTGGTATCAACGCAG	5	0.25075225677031093	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5	0.25075225677031093	No Hit
ACTCTGCGTTGATACCACTGCTTCC	4	0.20060180541624875	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4	0.20060180541624875	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	4	0.20060180541624875	No Hit
AAAGTACTCTGCGTTGATACCACTG	4	0.20060180541624875	No Hit
GTCCTATTCCATTATTCCTAGCTGC	4	0.20060180541624875	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4	0.20060180541624875	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3	0.15045135406218654	No Hit
GAATAACGCCGCCGCATCGCCAGTC	3	0.15045135406218654	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	3	0.15045135406218654	No Hit
GAACGAGACTCTGGCATGCTAACTA	3	0.15045135406218654	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3	0.15045135406218654	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3	0.15045135406218654	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	3	0.15045135406218654	No Hit
CCCATGTACTCTGCGTTGATACCAC	3	0.15045135406218654	No Hit
GCTTCCCATGTACTCTGCGTTGATA	3	0.15045135406218654	No Hit
CTGTAGACCAGGCTGGCCTCGAACT	3	0.15045135406218654	No Hit
TCGTAGTTCCGACCATAAACGATGC	3	0.15045135406218654	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3	0.15045135406218654	No Hit
GTACATGGGTACCTGGTTGATCCTG	3	0.15045135406218654	No Hit
GTATCAACGCAGAGTACATGGGAAT	3	0.15045135406218654	No Hit
TAACAATACAGGACTCTTTCGAGGC	2	0.10030090270812438	No Hit
ATCAGACATTAATTGAATTTATTAA	2	0.10030090270812438	No Hit
GGTTATCTAGAGTCACCAAAGCCGC	2	0.10030090270812438	No Hit
CCATAGACGCTTTCATCACTGTAGG	2	0.10030090270812438	No Hit
CCCTGATGGAGTCTGTGTTACTCAG	2	0.10030090270812438	No Hit
GCTCCACTCCTGGTGGTGCCCTTCC	2	0.10030090270812438	No Hit
GTAGTCGCCGTGCCTACCATGGTGA	2	0.10030090270812438	No Hit
GACCAGAGCGAAAGCATTTGCCAAG	2	0.10030090270812438	No Hit
GTATCAACGCAGAGTACATGGGCGA	2	0.10030090270812438	No Hit
AAATAGAACCGCGGTCCTATTCCAT	2	0.10030090270812438	No Hit
CTCCAATAGCGTATATTAAAGTTGC	2	0.10030090270812438	No Hit
GATACCACTGCTTCCCATGTACTCT	2	0.10030090270812438	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	2	0.10030090270812438	No Hit
GTGGTAGCCGTTTCTCAGGCTCCCT	2	0.10030090270812438	No Hit
CAGTGGTATCAACGCAGAGTACATG	2	0.10030090270812438	No Hit
ATTCCTAGCTGCGGTATCCAGGCGG	2	0.10030090270812438	No Hit
CTTAGTTGGTGGAGCGATTTGTCTG	2	0.10030090270812438	No Hit
CCTGGATACCGCAGCTAGGAATAAT	2	0.10030090270812438	No Hit
GATTGATAGCTCTTTCTCGATTCCG	2	0.10030090270812438	No Hit
CCATCCTACAGCCACCAGGTCTAAT	2	0.10030090270812438	No Hit
AATAAGATATGTTGATTTTACTTTG	2	0.10030090270812438	No Hit
CATACACACAGTCCGAGTTCGAGGA	2	0.10030090270812438	No Hit
ATACCACTGCTTCCATGTACTCTCA	2	0.10030090270812438	No Hit
GAACTACGACGGTATCTGATCGTCT	2	0.10030090270812438	No Hit
CCACTGCTTCCATGTACTCTGCGTT	2	0.10030090270812438	No Hit
GTACAGTGAAACTGCGAATGGCTCA	2	0.10030090270812438	No Hit
CCTCGAAAGAGTCCTGTATTGTTAT	2	0.10030090270812438	No Hit
GGTATCAACGCAGAGTACATGGGGG	2	0.10030090270812438	No Hit
GGTATCAACGCAGAGTACATGGGAA	2	0.10030090270812438	No Hit
AAAGTGGACTCATTCCAATTACAGG	2	0.10030090270812438	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	2	0.10030090270812438	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	2	0.10030090270812438	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	2	0.10030090270812438	No Hit
GGATTATCCGCGGCAACGCAACTGA	2	0.10030090270812438	No Hit
CTCCAGCGTGGCCCGAAATCCTTCT	2	0.10030090270812438	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2	0.10030090270812438	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2	0.10030090270812438	No Hit
ATCCTAGCCCTAGCCCTACACAAAT	2	0.10030090270812438	No Hit
ACTCACGCCACCCACCGGAGAATGG	2	0.10030090270812438	No Hit
CAAATTACCCACTCCCGACCCGGGG	2	0.10030090270812438	No Hit
TTCCAGAAGAGCTGTCCCTCTTTTG	2	0.10030090270812438	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2	0.10030090270812438	No Hit
CCCAAGATCCAACTACGAGCTTTTT	2	0.10030090270812438	No Hit
GTATCAACGCAGAGTACATGGGAAG	2	0.10030090270812438	No Hit
GCGTTGATACCACTGCTTCCATGTA	2	0.10030090270812438	No Hit
CGCTTAAACTCATCCAACACAAAGG	2	0.10030090270812438	No Hit
GGAGTGGAGCCTGCGGCTTAATTTG	2	0.10030090270812438	No Hit
GTACATGGAAGCAGTGGTATCAACT	2	0.10030090270812438	No Hit
GGCTATGACGAGCATGAAGGGCCAG	2	0.10030090270812438	No Hit
ACCTCGGAGTTGGGCGTCCGAGCGT	2	0.10030090270812438	No Hit
GGTTAATTCCGATAACGAACGAGAC	2	0.10030090270812438	No Hit
CCATGTACTCTGCGTTGATACCACT	2	0.10030090270812438	No Hit
CTGTCAATCCTGTCCGTGTCCGGGC	2	0.10030090270812438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE