##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062633_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1994
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.82848545636911	32.0	32.0	32.0	21.0	32.0
2	30.13139418254764	32.0	32.0	32.0	21.0	32.0
3	30.81594784353059	32.0	32.0	32.0	32.0	32.0
4	31.213640922768306	32.0	32.0	32.0	32.0	32.0
5	30.939317953861586	32.0	32.0	32.0	32.0	32.0
6	34.281344032096285	36.0	36.0	36.0	32.0	36.0
7	34.493480441323975	36.0	36.0	36.0	32.0	36.0
8	34.36710130391174	36.0	36.0	36.0	32.0	36.0
9	34.841524573721166	36.0	36.0	36.0	32.0	36.0
10	34.4679037111334	36.0	36.0	36.0	32.0	36.0
11	34.7542627883651	36.0	36.0	36.0	32.0	36.0
12	34.45837512537613	36.0	36.0	36.0	32.0	36.0
13	34.75125376128385	36.0	36.0	36.0	32.0	36.0
14	34.54262788365095	36.0	36.0	36.0	32.0	36.0
15	34.69859578736209	36.0	36.0	36.0	32.0	36.0
16	34.59929789368104	36.0	36.0	36.0	32.0	36.0
17	34.44433299899699	36.0	36.0	36.0	32.0	36.0
18	34.53961885656971	36.0	36.0	36.0	32.0	36.0
19	34.59779338014042	36.0	36.0	36.0	32.0	36.0
20	34.48094282848545	36.0	36.0	36.0	32.0	36.0
21	34.66950852557673	36.0	36.0	36.0	32.0	36.0
22	34.59227683049147	36.0	36.0	36.0	32.0	36.0
23	34.39769307923771	36.0	36.0	36.0	32.0	36.0
24	34.48094282848545	36.0	36.0	36.0	32.0	36.0
25	34.40672016048144	36.0	36.0	36.0	32.0	36.0
26	34.39167502507522	36.0	36.0	36.0	32.0	36.0
27	34.21514543630893	36.0	36.0	36.0	32.0	36.0
28	34.30541624874624	36.0	36.0	36.0	32.0	36.0
29	34.28986960882648	36.0	36.0	36.0	32.0	36.0
30	34.130391173520565	36.0	36.0	36.0	32.0	36.0
31	34.25877632898696	36.0	36.0	36.0	32.0	36.0
32	34.021564694082244	36.0	36.0	36.0	32.0	36.0
33	33.86710130391174	36.0	36.0	36.0	32.0	36.0
34	33.816950852557675	36.0	36.0	36.0	32.0	36.0
35	34.13390170511535	36.0	36.0	36.0	32.0	36.0
36	33.89267803410231	36.0	36.0	36.0	32.0	36.0
37	33.70812437311936	36.0	36.0	36.0	32.0	36.0
38	33.90320962888666	36.0	36.0	36.0	32.0	36.0
39	33.890170511534606	36.0	36.0	36.0	32.0	36.0
40	33.74373119358074	36.0	36.0	36.0	32.0	36.0
41	33.68655967903711	36.0	36.0	36.0	27.0	36.0
42	33.57723169508525	36.0	36.0	36.0	21.0	36.0
43	33.67151454363089	36.0	36.0	36.0	27.0	36.0
44	33.2728184553661	36.0	36.0	36.0	21.0	36.0
45	33.28686058174524	36.0	36.0	36.0	21.0	36.0
46	33.3074222668004	36.0	36.0	36.0	21.0	36.0
47	33.41173520561685	36.0	36.0	36.0	21.0	36.0
48	33.11334002006018	36.0	36.0	36.0	14.0	36.0
49	33.42126379137412	36.0	36.0	36.0	21.0	36.0
50	32.47793380140421	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.0
24	3.0
25	7.0
26	8.0
27	23.0
28	40.0
29	74.0
30	74.0
31	123.0
32	164.0
33	266.0
34	424.0
35	785.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28585757271815	17.502507522567704	13.34002006018054	23.8716148445336
2	18.56497742097341	21.12393376818866	37.63171098845961	22.679377822378324
3	23.470411233701103	23.269809428284855	28.284854563691077	24.974924774322968
4	15.496489468405217	17.101303911735204	33.09929789368105	34.302908726178536
5	16.850551654964896	35.30591775325978	32.59779338014042	15.245737211634905
6	34.60381143430291	36.308926780341025	16.29889669007021	12.788365095285858
7	29.789368104312942	31.444332998996995	19.90972918756269	18.85656970912738
8	27.9839518555667	31.39418254764293	18.906720160481445	21.715145436308926
9	27.5827482447342	14.092276830491473	18.706118355065197	39.61885656970912
10	16.0481444332999	28.13440320962889	29.087261785356066	26.730190571715145
11	37.71313941825476	19.45837512537613	22.26680040120361	20.561685055165498
12	23.42026078234704	25.22567703109328	27.933801404212637	23.42026078234704
13	29.38816449348044	19.45837512537613	24.57372116349047	26.57973921765296
14	23.620862587763288	19.408224674022065	24.2728184553661	32.698094282848544
15	25.325977933801404	26.78034102306921	23.971915747241727	23.921765295887663
16	25.877632898696092	25.325977933801404	23.570712136409227	25.22567703109328
17	24.924774322968908	28.084252758274825	23.570712136409227	23.42026078234704
18	24.172517552657975	25.075225677031092	27.23169508525577	23.520561685055167
19	26.37913741223671	24.924774322968908	24.623871614844532	24.072216649949848
20	24.82447342026078	25.426278836509532	26.178535606820464	23.570712136409227
21	24.12236710130391	26.028084252758276	24.022066198595788	25.827482447342025
22	26.028084252758276	27.28184553660983	23.269809428284855	23.42026078234704
23	23.771313941825476	26.479438314944836	25.02507522567703	24.72417251755266
24	24.222668004012036	25.727181544633904	25.125376128385156	24.924774322968908
25	26.730190571715145	24.974924774322968	25.576730190571716	22.71815446339017
26	22.668004012036107	25.77733199598796	26.178535606820464	25.376128385155468
27	25.47642928786359	27.482447342026077	23.219658976930795	23.82146439317954
28	24.77432296890672	25.47642928786359	24.32296890672016	25.426278836509532
29	24.623871614844532	24.924774322968908	26.22868605817452	24.222668004012036
30	24.172517552657975	24.32296890672016	26.83049147442327	24.674022066198596
31	22.617853560682047	25.827482447342025	27.28184553660983	24.2728184553661
32	24.2728184553661	26.078234704112337	25.376128385155468	24.2728184553661
33	22.668004012036107	26.930792377131397	25.325977933801404	25.075225677031092
34	24.423269809428287	27.08124373119358	25.376128385155468	23.119358074222667
35	25.275827482447344	27.23169508525577	24.2728184553661	23.219658976930795
36	22.968906720160483	26.83049147442327	25.877632898696092	24.32296890672016
37	24.924774322968908	25.877632898696092	24.57372116349047	24.623871614844532
38	22.71815446339017	27.181544633901705	25.626880641925776	24.473420260782348
39	25.325977933801404	26.078234704112337	23.42026078234704	25.175526579739216
40	23.319959879638915	26.1283851554664	27.833500501504517	22.71815446339017
41	24.072216649949848	25.125376128385156	24.623871614844532	26.178535606820464
42	25.075225677031092	26.629889669007024	25.47642928786359	22.818455366098295
43	22.517552657973923	26.32898696088265	25.977933801404212	25.175526579739216
44	24.022066198595788	26.78034102306921	25.626880641925776	23.570712136409227
45	22.517552657973923	26.78034102306921	25.376128385155468	25.325977933801404
46	21.715145436308926	27.73319959879639	27.783350050150453	22.768304914744235
47	25.526579739217652	24.674022066198596	27.331995987963893	22.46740220661986
48	25.47642928786359	29.187562688064194	22.417251755265795	22.91875626880642
49	23.570712136409227	26.078234704112337	27.13139418254764	23.219658976930795
50	22.517552657973923	28.43530591775326	24.674022066198596	24.37311935807422
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.0
11	3.0
12	3.0
13	4.0
14	5.0
15	5.0
16	5.0
17	5.0
18	5.0
19	7.0
20	9.0
21	11.0
22	13.0
23	12.0
24	11.0
25	14.5
26	18.0
27	18.5
28	19.0
29	21.0
30	23.0
31	32.0
32	41.0
33	46.0
34	51.0
35	59.5
36	68.0
37	77.0
38	86.0
39	92.5
40	99.0
41	109.0
42	119.0
43	108.5
44	98.0
45	130.0
46	162.0
47	160.5
48	159.0
49	157.5
50	156.0
51	133.0
52	110.0
53	114.5
54	119.0
55	120.5
56	122.0
57	120.0
58	118.0
59	111.0
60	104.0
61	81.5
62	59.0
63	55.5
64	52.0
65	45.5
66	39.0
67	34.5
68	30.0
69	29.5
70	29.0
71	21.5
72	14.0
73	12.5
74	11.0
75	9.0
76	7.0
77	6.5
78	6.0
79	5.0
80	4.0
81	4.0
82	4.0
83	2.5
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	4.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.05015045135406219
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1994.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.58676028084253
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.32738719832109	92.07622868605817
2	3.04302203567681	5.817452357071214
3	0.5246589716684155	1.5045135406218655
4	0.05246589716684155	0.20060180541624875
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.05246589716684155	0.4012036108324975
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	8	0.4012036108324975	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	4	0.20060180541624875	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	3	0.15045135406218654	No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG	3	0.15045135406218654	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	3	0.15045135406218654	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3	0.15045135406218654	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	3	0.15045135406218654	No Hit
TTAGGGACTAGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTT	3	0.15045135406218654	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	3	0.15045135406218654	No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA	3	0.15045135406218654	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	3	0.15045135406218654	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3	0.15045135406218654	No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGC	2	0.10030090270812438	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	2	0.10030090270812438	No Hit
GGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGA	2	0.10030090270812438	No Hit
GCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTG	2	0.10030090270812438	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA	2	0.10030090270812438	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.10030090270812438	No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG	2	0.10030090270812438	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	2	0.10030090270812438	No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG	2	0.10030090270812438	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2	0.10030090270812438	No Hit
GGTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATA	2	0.10030090270812438	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	2	0.10030090270812438	No Hit
CCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGTTCTATTTTG	2	0.10030090270812438	No Hit
CTTTTGTGCCGTCTGGTGCGCTCCCGACGATCCTTGAAAATCCACCGGAA	2	0.10030090270812438	No Hit
GATAAATGCACGCATCCCCCCCCGGGAAGGGGGGTCAGCGCCCGTCGGCA	2	0.10030090270812438	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACAC	2	0.10030090270812438	No Hit
ACCCGGGGACGGGTCCATACGGCGTTGTTCTTGATTCCCATCGTAACTTA	2	0.10030090270812438	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	2	0.10030090270812438	No Hit
GTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCAGTTTTCTTGCCA	2	0.10030090270812438	No Hit
GCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAGTCACCAAAGCC	2	0.10030090270812438	No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA	2	0.10030090270812438	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	2	0.10030090270812438	No Hit
GTATTGTAAACAGCGTTCTTTAATATGTCTGCTTTTCCATCTCAGCTCTA	2	0.10030090270812438	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2	0.10030090270812438	No Hit
GGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATG	2	0.10030090270812438	No Hit
CAATTACAGGGCCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACC	2	0.10030090270812438	No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG	2	0.10030090270812438	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCATCGCAGAGTA	2	0.10030090270812438	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCATGGTGAC	2	0.10030090270812438	No Hit
GTTCATATTGTTGTTCCAGCTATAGGGTTGCAGATCCCCTCAGTTCCTTG	2	0.10030090270812438	No Hit
GGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTT	2	0.10030090270812438	No Hit
CAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCT	2	0.10030090270812438	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	2	0.10030090270812438	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	2	0.10030090270812438	No Hit
TGGTGGCGCAGGCCTTTAATCCCAGCACTCAGGAGGCAGAGGCAGGCACA	2	0.10030090270812438	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2	0.10030090270812438	No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTA	2	0.10030090270812438	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2	0.10030090270812438	No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC	2	0.10030090270812438	No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT	2	0.10030090270812438	No Hit
CCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCATA	2	0.10030090270812438	No Hit
AAAAAGACCTGTGGCATACTTGTATAAAAAATTAGACCTGGTGGCTGTAG	2	0.10030090270812438	No Hit
ATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCC	2	0.10030090270812438	No Hit
TCTCTGTGCATAATCTTGTGCTTCTCCCGAAGATAGGTCAGGCCTTTTAT	2	0.10030090270812438	No Hit
TGCCAGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCA	2	0.10030090270812438	No Hit
GGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGA	2	0.10030090270812438	No Hit
GATATAGAAAGACCAGTCCTTGCTGAAGGACATATCTGACATCTCTACTT	2	0.10030090270812438	No Hit
GTTCGAAGACGATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGA	2	0.10030090270812438	No Hit
CACTGAAGGTGGGCAGATTCAAGATGAAGCGCTTCTGGAGCTCCTCCAGA	2	0.10030090270812438	No Hit
GAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGC	2	0.10030090270812438	No Hit
CGATGGGTCAGCTGTACCAGGAACACCATGAAGAAGACTTCTTTCTATAC	2	0.10030090270812438	No Hit
AGTGTGAGCAGGCATTGGCTGCTGAGCCCAAGGCCAAGGACCCTTTCGCT	2	0.10030090270812438	No Hit
GTGAGGCCACATTCTGGGATCCAGGAAAGCCGTCCAGGCAGAAGTAGAAC	2	0.10030090270812438	No Hit
GTGGAAATGCTTCCGCTGAAGGTCCGGAGGGCGAAGGTACCGAAAGCACA	2	0.10030090270812438	No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT	2	0.10030090270812438	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGG	2	0.10030090270812438	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	2	0.10030090270812438	No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG	2	0.10030090270812438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.05015045135406219	0.0
31	0.0	0.0	0.0	0.20060180541624875	0.0
32	0.0	0.0	0.0	0.20060180541624875	0.0
33	0.0	0.0	0.0	0.20060180541624875	0.0
34	0.0	0.0	0.0	0.30090270812437314	0.0
35	0.0	0.0	0.0	0.4012036108324975	0.0
36	0.0	0.0	0.0	0.4012036108324975	0.0
37	0.0	0.0	0.0	0.6018054162487463	0.0
38	0.0	0.0	0.0	0.6519558676028084	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE