FastQCFastQC Report
Thu 2 Feb 2017
SRR4062632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000783
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG221982.218063256470184No Hit
TCCATGTACTCTGCGTTGATACCAC183541.8339640061831586No Hit
GAGTACATGGAAGCAGTGGTATCAA97130.9705400671274391No Hit
CATGTACTCTGCGTTGATACCACTG80810.8074677527495971No Hit
GCTTCCATGTACTCTGCGTTGATAC58790.5874400344530233No Hit
GTCCTACAGTGGACATTTCTAAATT57600.5755493448629723No Hit
CCCATGTACTCTGCGTTGATACCAC56170.5612605330026589No Hit
GTCCTAAAGTGTGTATTTCTCATTT55690.5564642884621341No Hit
CTGTAGGACGTGGAATATGGCAAGA53770.5372793103000351No Hit
GTACATGGGAAGCAGTGGTATCAAC52040.5199928456018937No Hit
GCGTTGATACCACTGCTTCCATGTA51120.5108000435658879No Hit
CATGGAAGCAGTGGTATCAACGCAG51010.5097009041920176No Hit
CTTTAGGACGTGAAATATGGCGAGG45240.45204604794445946No Hit
GTATCAACGCAGAGTACATGGAAGC40010.3997869668049917No Hit
GTCCTACAGTGTGCATTTCTCATTT39730.39698915748968555No Hit
GTACTTTTTTTTTTTTTTTTTTTTT39200.39169330414285614No Hit
GATATACACTGTTCTACAAATCCCG38520.38489862437711275No Hit
GTATCAACGCAGAGTACTTTTTTTT37420.37390723063841014No Hit
TTGTAGAACAGTGTATATCAATGAG36960.36931082962040723No Hit
GTGTATATCAATGAGTTACAATGAA34750.3472281203817411No Hit
ACTCTGCGTTGATACCACTGCTTCC32630.32604470699442334No Hit
ACGCAGAGTACATGGAAGCAGTGGT31820.3179510443322878No Hit
CAGTGGTATCAACGCAGAGTACATG31630.31605253086833013No Hit
CTGTAGGACCTGGAATATGGCGAGA31210.3118558168953709No Hit
CTGAAGGACCTGGAATATGGCGAGA28420.2839776455035707No Hit
TATCAACGCAGAGTACTTTTTTTTT26840.26819000722434333No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26200.2617950145036436No Hit
GTATCAACGCAGAGTACATGGGAAG23940.23921269645867288No Hit
GATATACACTGTTCTACAATGCCGG23790.23771387003975888No Hit
GTCCTTCAGTGTGCATTTCTCATTT23630.23611512185958394No Hit
TATCAACGCAGAGTACATGGAAGCA23590.23571543481454021No Hit
GTGTATATCAATGAGTTACAATGAG23330.23311746902175598No Hit
ATTTAGAAATGTCCACTGTAGGACG22910.22892075504879678No Hit
GTACATGGGGTGGTATCAACGCAAA22710.22692231982357813No Hit
GTTCTACAGTGTGGTTTTTATCATT22660.2264227110172735No Hit
GTTCTACAAATCCCGTTTCCAACGA18170.18155784021111468No Hit
GGTATCAACGCAGAGTACATGGAAG18070.18055862259850536No Hit
TTTCTAAATTTTCCACCTTTTTCAG18030.18015893555346163No Hit
CTGTAGAACATATTAGATGAGTGAG17810.17796065680572112No Hit
AAAAAGTACTCTGCGTTGATACCAC17650.17636190862554618No Hit
GAGTACATGGGAAGCAGTGGTATCA17190.17176550760754328No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17000.16986699414358558No Hit
ATACCACTGCTTCCATGTACTCTGC16970.16956722885980277No Hit
AAGCAGTGGTATCAACGCAGAGTAC16640.166269810738192No Hit
GCGTTGATACCACTGCTTCCCATGT16570.16557035840936546No Hit
GAATATGGCAAGAAAACTGAAAATC15980.15967497449497042No Hit
ATGTTCTACAGTGTGGTTTTTATCA15950.15937520921118764No Hit
CTGTAGGACATGGAATATGGCAAGA15600.155877947567055No Hit
GGAATATGGCGAGAAAACTGAAAAT14940.14928311132383343No Hit
GCTTCCCATGTACTCTGCGTTGATA14780.14768436314365851No Hit
GTACTCTGCGTTGATACCACTGCTT14750.14738459785987573No Hit
GGTATCAACGCAGAGTACTTTTTTT13600.13589359531486847No Hit
GTAGAACAGTGTATATCAATGAGTT13470.13459461241847634No Hit
GTGGTATCAACGCAGAGTACATGGA13400.1338951600896498No Hit
CCACTGCTTCCATGTACTCTGCGTT12980.12969844611669062No Hit
GTACATGGGTGGTATCAACGCAAAA12930.12919883731038598No Hit
GTAGAACATATTAGATGAGTGAGTT12780.12770001089147198No Hit
ACATGGAAGCAGTGGTATCAACGCA12140.12130501817077229No Hit
ATTCCAGGTCCTTCAGTGTGCATTT11970.11960634822933644No Hit
GAAGCAGTGGTATCAACGCAGAGTA11820.11810752181042243No Hit
TATCAACGCAGAGTACATGGGAAGC11680.11670861715276938No Hit
GGTATCAACGCAGAGTACATGGGAA11540.11530971249511632No Hit
ACGCAGAGTACATGGGAAGCAGTGG11530.11520979073385539No Hit
GCCATATTCCACGTCCTACAGTGGA11420.11411065135998513No Hit
ACCTGGAATATGGCGAGAAAACTGA11380.1137109643149414No Hit
ACAGTGGACATTTCTAAATTTTCCA11220.11211221613476649No Hit
TGGAAGCAGTGGTATCAACGCAGAG11030.11021370267080875No Hit
CCATAGGGTCTTCTCGTCTTATTAT10890.10881479801315569No Hit
CTGCTTCCATGTACTCTGCGTTGAT10810.10801542392306825No Hit
CCATATTCCAGGTCCTTCAGTGTGC10790.10781558040054637No Hit
GTACATGGGAGTGGTATCAACGCAA10780.10771565863928544No Hit
CATCTAATATGTTCTACAGTGTGGT10610.10601698869784958No Hit
GTCCACTGTAGGACGTGGAATATGG10540.10531753636902307No Hit
GACCTGGAATATGGCGAGAAAACTG10490.10481792756271838No Hit
GAAATATGGCGAGGAAAACTGAAAA10390.10381870995010906No Hit
ATACACACTTTAGGACGTGAAATAT10080.10072113535102015No Hit
TTCCAGGTCCTTCAGTGTGCATTTC10070.10062121358975923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA659.998985E-917.5405866
GATCGGT453.5331777E-516.8909347
ACAGTTC402.7573324E-416.6270148
GACCGTT402.7573324E-416.6270147
GGAACGC402.7573324E-416.6270148
GACCTAG753.272362E-916.468667
GAAAGCG601.4636844E-615.8352519
AACCTAT601.4636844E-615.8352516
GGATCGG551.126212E-515.5473376
TCGATGG508.707422E-515.20031913
CGATGGT508.707422E-515.20031914
AAGACGT508.711138E-515.1995595
CTAACAC456.771092E-414.7743943
GTCCTAG1350.014.7699621
TAGGACC11650.014.6769524
CCAACGA3550.014.45317719
GGACCGT400.005273121414.2517266
GCGTATG801.2849341E-714.2517268
GGTCGAT602.5647323E-514.25101411
AGGATCG602.567153E-514.2495885
Produced by FastQC (version 0.11.5)