##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062632_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1000783
Sequences flagged as poor quality	0
Sequence length	25
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08397424816369	32.0	32.0	32.0	32.0	32.0
2	30.995240726511142	32.0	32.0	32.0	32.0	32.0
3	30.97370758695941	32.0	32.0	32.0	32.0	32.0
4	30.979025423093717	32.0	32.0	32.0	32.0	32.0
5	30.96981263670546	32.0	32.0	32.0	32.0	32.0
6	34.581475704523356	36.0	36.0	36.0	32.0	36.0
7	34.385075485894546	36.0	36.0	36.0	32.0	36.0
8	34.39544236862537	36.0	36.0	36.0	32.0	36.0
9	34.42404197513347	36.0	36.0	36.0	32.0	36.0
10	34.288734920557204	36.0	36.0	36.0	32.0	36.0
11	34.521011048349145	36.0	36.0	36.0	32.0	36.0
12	34.364764389483035	36.0	36.0	36.0	32.0	36.0
13	34.39373270729019	36.0	36.0	36.0	32.0	36.0
14	34.34324024288982	36.0	36.0	36.0	32.0	36.0
15	34.34225901119424	36.0	36.0	36.0	32.0	36.0
16	34.329974629864815	36.0	36.0	36.0	32.0	36.0
17	34.280276543466464	36.0	36.0	36.0	32.0	36.0
18	34.3080697813612	36.0	36.0	36.0	32.0	36.0
19	34.22086506265594	36.0	36.0	36.0	32.0	36.0
20	34.17166058975822	36.0	36.0	36.0	32.0	36.0
21	34.011785771740726	36.0	36.0	36.0	32.0	36.0
22	33.96574781945736	36.0	36.0	36.0	32.0	36.0
23	33.87564137280509	36.0	36.0	36.0	32.0	36.0
24	33.85466379824597	36.0	36.0	36.0	32.0	36.0
25	33.3128090704978	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	12.0
4	32.0
5	102.0
6	173.0
7	38.0
8	142.0
9	184.0
10	134.0
11	42.0
12	92.0
13	58.0
14	265.0
15	359.0
16	506.0
17	659.0
18	894.0
19	1209.0
20	1659.0
21	2343.0
22	3323.0
23	4354.0
24	5845.0
25	7527.0
26	10064.0
27	13016.0
28	17382.0
29	23597.0
30	30715.0
31	43616.0
32	63900.0
33	93192.0
34	213695.0
35	461653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.396666300252825	18.769549011182285	12.991435909222638	23.842348779342252
2	10.208774045787797	22.366891931727	46.004234836577076	21.420099185908125
3	20.807327421672493	28.538952408931845	29.194571184750394	21.45914898464527
4	10.57728342502511	18.827144763075445	38.85332813663324	31.742243675266206
5	9.507306931088943	41.484576477879294	37.00835649027409	11.999760100757682
6	28.629981146070964	37.48852869376061	20.76881244239355	13.112677717774876
7	24.212130362703004	32.98993502662728	23.53079985841444	19.26713475225527
8	23.62925914035598	41.0025613099185	22.128977606290363	13.239201943435155
9	26.11087050238103	16.805614040702995	20.13835057547718	36.9451648814388
10	14.697643615031083	30.701133073624774	36.03836403616712	18.562859275177026
11	33.083389774890726	21.644745332691716	26.432156581052247	18.83970831136531
12	22.966961498439126	25.026915072440563	34.937565036420395	17.068558392699913
13	32.02880121975987	23.1572291681882	26.159607259732216	18.654362352319716
14	20.78384251514739	24.74917759866815	26.63159621895485	27.835383667229607
15	22.38100192269602	34.769086409280156	26.76758986753205	16.082321800491776
16	20.030110990511538	27.549055405222905	34.9207460723164	17.500087531949163
17	18.799448537503853	29.40309587363585	33.23221853602315	18.56523705283715
18	19.951813883124654	26.080435596093082	38.54539160224842	15.422358918533845
19	22.338667072233605	30.025194512038933	26.818747598616806	20.817390817110656
20	20.70954057947815	28.58912277893389	34.353789819113175	16.347546822474786
21	20.061394565140173	29.280284800342592	25.317954008106565	25.34036662641067
22	19.557483244749253	34.065523863660246	27.335001936473656	19.041990955116844
23	18.37762492470196	32.320720619755406	28.036287151202465	21.26536730434017
24	20.88817155453537	31.746514585557634	29.393453334520736	17.971860525386266
25	22.236908194772685	29.65234600907306	28.910231909405447	19.200513886748805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	391.0
1	391.0
2	845.5
3	1300.0
4	1300.0
5	1300.0
6	3670.5
7	6041.0
8	6041.0
9	6041.0
10	5655.0
11	5269.0
12	5269.0
13	5269.0
14	4729.0
15	4189.0
16	4189.0
17	4189.0
18	7325.5
19	10462.0
20	10462.0
21	10462.0
22	19456.5
23	28451.0
24	28451.0
25	28451.0
26	48283.0
27	68115.0
28	68115.0
29	68115.0
30	94469.0
31	120823.0
32	120823.0
33	120823.0
34	119424.0
35	118025.0
36	118025.0
37	118025.0
38	114490.0
39	110955.0
40	110955.0
41	110955.0
42	117953.5
43	124952.0
44	124952.0
45	124952.0
46	165324.0
47	205696.0
48	205696.0
49	205696.0
50	155361.5
51	105027.0
52	105027.0
53	105027.0
54	72379.5
55	39732.0
56	39732.0
57	39732.0
58	30835.0
59	21938.0
60	21938.0
61	21938.0
62	17389.5
63	12841.0
64	12841.0
65	12841.0
66	10400.0
67	7959.0
68	7959.0
69	7959.0
70	6177.0
71	4395.0
72	4395.0
73	4395.0
74	3261.0
75	2127.0
76	2127.0
77	2127.0
78	1556.0
79	985.0
80	985.0
81	985.0
82	698.0
83	411.0
84	411.0
85	411.0
86	274.5
87	138.0
88	138.0
89	138.0
90	101.0
91	64.0
92	64.0
93	64.0
94	52.5
95	41.0
96	41.0
97	41.0
98	248.5
99	456.0
100	456.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00929272379726674
2	0.003597183405393577
3	0.004896166301785702
4	0.014788420666618039
5	0.03627159933771857
6	0.04606393194128997
7	0.06804671941869515
8	0.09032927217988315
9	0.10032144830597642
10	0.12000603527438015
11	0.1223042357833816
12	0.13419492537343258
13	0.1241028274860784
14	0.12700055856264544
15	0.11441041664376793
16	0.11471018192755073
17	0.12580149742751426
18	0.13239633367073583
19	0.13579367355360752
20	0.126201184472558
21	0.1344946906572154
22	0.1542791993868801
23	0.14198882275178534
24	0.1402901528103495
25	0.13399508185091075
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1000783.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.86453983517514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.73560985634427	20.70615368299032
2	14.538329616604189	8.392843887107563
3	5.119069219732736	4.432787322359834
4	2.4766503549577665	2.859494913139164
5	1.37895278263857	1.9901418762648306
6	0.8511522262898996	1.474087040492569
7	0.6042429298739102	1.2208835883627893
8	0.41969737292472753	0.9691497231603323
9	0.31843704970868825	0.827238501568087
>10	2.0386804270982424	11.353330717825568
>50	0.24140573085970604	4.81316358001199
>100	0.22581998291113034	13.073269540312637
>500	0.025283525694037377	4.961522180456935
>1k	0.022512728357704515	12.732264237817292
>5k	0.0034634966704160785	6.139595028479352
>10k+	6.926993340832158E-4	4.054074179650802
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	22198	2.218063256470184	No Hit
TCCATGTACTCTGCGTTGATACCAC	18354	1.8339640061831586	No Hit
GAGTACATGGAAGCAGTGGTATCAA	9713	0.9705400671274391	No Hit
CATGTACTCTGCGTTGATACCACTG	8081	0.8074677527495971	No Hit
GCTTCCATGTACTCTGCGTTGATAC	5879	0.5874400344530233	No Hit
GTCCTACAGTGGACATTTCTAAATT	5760	0.5755493448629723	No Hit
CCCATGTACTCTGCGTTGATACCAC	5617	0.5612605330026589	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5569	0.5564642884621341	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5377	0.5372793103000351	No Hit
GTACATGGGAAGCAGTGGTATCAAC	5204	0.5199928456018937	No Hit
GCGTTGATACCACTGCTTCCATGTA	5112	0.5108000435658879	No Hit
CATGGAAGCAGTGGTATCAACGCAG	5101	0.5097009041920176	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4524	0.45204604794445946	No Hit
GTATCAACGCAGAGTACATGGAAGC	4001	0.3997869668049917	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3973	0.39698915748968555	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3920	0.39169330414285614	No Hit
GATATACACTGTTCTACAAATCCCG	3852	0.38489862437711275	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3742	0.37390723063841014	No Hit
TTGTAGAACAGTGTATATCAATGAG	3696	0.36931082962040723	No Hit
GTGTATATCAATGAGTTACAATGAA	3475	0.3472281203817411	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3263	0.32604470699442334	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	3182	0.3179510443322878	No Hit
CAGTGGTATCAACGCAGAGTACATG	3163	0.31605253086833013	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3121	0.3118558168953709	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2842	0.2839776455035707	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2684	0.26819000722434333	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2620	0.2617950145036436	No Hit
GTATCAACGCAGAGTACATGGGAAG	2394	0.23921269645867288	No Hit
GATATACACTGTTCTACAATGCCGG	2379	0.23771387003975888	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2363	0.23611512185958394	No Hit
TATCAACGCAGAGTACATGGAAGCA	2359	0.23571543481454021	No Hit
GTGTATATCAATGAGTTACAATGAG	2333	0.23311746902175598	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2291	0.22892075504879678	No Hit
GTACATGGGGTGGTATCAACGCAAA	2271	0.22692231982357813	No Hit
GTTCTACAGTGTGGTTTTTATCATT	2266	0.2264227110172735	No Hit
GTTCTACAAATCCCGTTTCCAACGA	1817	0.18155784021111468	No Hit
GGTATCAACGCAGAGTACATGGAAG	1807	0.18055862259850536	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1803	0.18015893555346163	No Hit
CTGTAGAACATATTAGATGAGTGAG	1781	0.17796065680572112	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1765	0.17636190862554618	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1719	0.17176550760754328	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1700	0.16986699414358558	No Hit
ATACCACTGCTTCCATGTACTCTGC	1697	0.16956722885980277	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1664	0.166269810738192	No Hit
GCGTTGATACCACTGCTTCCCATGT	1657	0.16557035840936546	No Hit
GAATATGGCAAGAAAACTGAAAATC	1598	0.15967497449497042	No Hit
ATGTTCTACAGTGTGGTTTTTATCA	1595	0.15937520921118764	No Hit
CTGTAGGACATGGAATATGGCAAGA	1560	0.155877947567055	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1494	0.14928311132383343	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1478	0.14768436314365851	No Hit
GTACTCTGCGTTGATACCACTGCTT	1475	0.14738459785987573	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1360	0.13589359531486847	No Hit
GTAGAACAGTGTATATCAATGAGTT	1347	0.13459461241847634	No Hit
GTGGTATCAACGCAGAGTACATGGA	1340	0.1338951600896498	No Hit
CCACTGCTTCCATGTACTCTGCGTT	1298	0.12969844611669062	No Hit
GTACATGGGTGGTATCAACGCAAAA	1293	0.12919883731038598	No Hit
GTAGAACATATTAGATGAGTGAGTT	1278	0.12770001089147198	No Hit
ACATGGAAGCAGTGGTATCAACGCA	1214	0.12130501817077229	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	1197	0.11960634822933644	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1182	0.11810752181042243	No Hit
TATCAACGCAGAGTACATGGGAAGC	1168	0.11670861715276938	No Hit
GGTATCAACGCAGAGTACATGGGAA	1154	0.11530971249511632	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1153	0.11520979073385539	No Hit
GCCATATTCCACGTCCTACAGTGGA	1142	0.11411065135998513	No Hit
ACCTGGAATATGGCGAGAAAACTGA	1138	0.1137109643149414	No Hit
ACAGTGGACATTTCTAAATTTTCCA	1122	0.11211221613476649	No Hit
TGGAAGCAGTGGTATCAACGCAGAG	1103	0.11021370267080875	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	1089	0.10881479801315569	No Hit
CTGCTTCCATGTACTCTGCGTTGAT	1081	0.10801542392306825	No Hit
CCATATTCCAGGTCCTTCAGTGTGC	1079	0.10781558040054637	No Hit
GTACATGGGAGTGGTATCAACGCAA	1078	0.10771565863928544	No Hit
CATCTAATATGTTCTACAGTGTGGT	1061	0.10601698869784958	No Hit
GTCCACTGTAGGACGTGGAATATGG	1054	0.10531753636902307	No Hit
GACCTGGAATATGGCGAGAAAACTG	1049	0.10481792756271838	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1039	0.10381870995010906	No Hit
ATACACACTTTAGGACGTGAAATAT	1008	0.10072113535102015	No Hit
TTCCAGGTCCTTCAGTGTGCATTTC	1007	0.10062121358975923	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCTA	65	9.998985E-9	17.540586	6
GATCGGT	45	3.5331777E-5	16.890934	7
ACAGTTC	40	2.7573324E-4	16.627014	8
GACCGTT	40	2.7573324E-4	16.627014	7
GGAACGC	40	2.7573324E-4	16.627014	8
GACCTAG	75	3.272362E-9	16.46866	7
GAAAGCG	60	1.4636844E-6	15.835251	9
AACCTAT	60	1.4636844E-6	15.835251	6
GGATCGG	55	1.126212E-5	15.547337	6
TCGATGG	50	8.707422E-5	15.200319	13
CGATGGT	50	8.707422E-5	15.200319	14
AAGACGT	50	8.711138E-5	15.199559	5
CTAACAC	45	6.771092E-4	14.774394	3
GTCCTAG	135	0.0	14.769962	1
TAGGACC	1165	0.0	14.676952	4
CCAACGA	355	0.0	14.453177	19
GGACCGT	40	0.0052731214	14.251726	6
GCGTATG	80	1.2849341E-7	14.251726	8
GGTCGAT	60	2.5647323E-5	14.251014	11
AGGATCG	60	2.567153E-5	14.249588	5
>>END_MODULE