FastQCFastQC Report
Thu 2 Feb 2017
SRR4062632_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062632_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000783
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA78240.7817878601055375No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC70840.7078457567724471No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA31120.31095652104402255No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC29340.2931704475395765No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA28060.28038046209817713No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG21250.212333742679482No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC20710.2069379675713916No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA20460.20443992353986828No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG20400.20384039297230266No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA18900.1888521287831628No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC18560.18545478890029107No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA18280.18265697958498497No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG18030.18015893555346163No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC17530.17516284749041502No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA17080.17066636823367304No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA17050.17036660294989023No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT16440.16427137551297333No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA15160.15148139007157396No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA14140.14128937042295883No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14020.14009030928782762No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC13760.13749234349504338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.11461026016628978No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA11070.11061338971585248No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG10930.10921448505819943No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC10580.1057172234140668No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG10350.10341902290506534No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATA10190.10182027472489041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG353.2149628E-431.4327492
CGAGATT353.2177902E-431.42803628
GACGTTG300.005745025429.3328327
TAGGACC14600.026.5200984
GACCGTT508.340986E-526.3995517
TGCGCTA508.340986E-526.39955110
GGACCGT508.340986E-526.3995516
TGCACGA508.340986E-526.39955142
GCTATCG551.5935348E-423.99959213
CGCTATC551.5935348E-423.99959212
GCGCTAT651.8132256E-523.69190411
AGGACCT25500.023.2074495
ATACATG2850.023.1713921
ATACACT18200.022.9666424
GATATAC17950.022.9325791
GGACCTG25600.022.8589866
ACTGTTC16700.022.131368
ACGTTGA703.2150616E-521.9996268
GCACGAA500.002580385421.99962643
ACCGTTA500.002580385421.9996268
Produced by FastQC (version 0.11.5)