FastQCFastQC Report
Thu 2 Feb 2017
SRR4062631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620314
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12610.2032841431919963No Hit
GCGCAAGACGGACCAGAGCGAAAGC9700.15637241783999717No Hit
TATCAACGCAGAGTACTTTTTTTTT9070.14621627111430663No Hit
GAACTACGACGGTATCTGATCGTCT7790.12558156030655443No Hit
GTATCTGATCGTCTTCGAACCTCCG7510.12106771731735862No Hit
GAATAGGACCGCGGTTCTATTTTGT7370.11881079582276075No Hit
GAATAACGCCGCCGCATCGCCAGTC7240.11671508300634839No Hit
ATCAGATACCGTCGTAGTTCCGACC7110.11461937018993607No Hit
GATTAAGAGGGACGGCCGGGGGCAT6980.11252365737352372No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG6940.11187882266078146No Hit
GTGCATGGCCGTTCTTAGTTGGTGG6890.11107277926985365No Hit
GTCCTATTCCATTATTCCTAGCTGC6390.10301234536057545No Hit
TCGTAGTTCCGACCATAAACGATGC6360.10252871932601876No Hit
GTCTTGCGCCGGTCCAAGAATTTCA6340.10220630196964764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2200.016.3980161
GTCTTAG601.4736779E-615.8226471
CGTTTCC704.4209264E-714.92933314
CGGTCCA1550.014.71161510
TGAACGG400.00527540314.2495775
CCTAAAT400.005289544414.2438292
GTATCAA8000.013.8843741
TATACTG551.9576536E-413.8177715
CTTAGAC551.963022E-413.8133123
GTACTGG707.2964176E-613.5622691
GCATCGC1102.730303E-912.95520614
CGCATCG1051.9832441E-812.66731413
GAACCGC751.47441515E-512.6673126
AGAACCG604.0868818E-412.666295
TTAGGAC1207.403287E-1012.6622033
TCGCCAG1155.2732503E-912.39493717
CCGACCA1155.285983E-912.3929369
TAACTTT853.9302704E-612.29474456
CCAACGA701.08670225E-412.21688119
GATGCAA701.0875216E-412.21589516
Produced by FastQC (version 0.11.5)