##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062631_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620314
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.488381690563166	32.0	32.0	32.0	32.0	32.0
2	29.41369854622014	32.0	32.0	32.0	14.0	32.0
3	29.66388957850379	32.0	32.0	32.0	21.0	32.0
4	29.257095599970338	32.0	32.0	32.0	14.0	32.0
5	29.36361584616823	32.0	32.0	32.0	14.0	32.0
6	32.47985536357393	36.0	32.0	36.0	21.0	36.0
7	32.54391163185097	36.0	32.0	36.0	21.0	36.0
8	32.196013631805826	36.0	32.0	36.0	21.0	36.0
9	32.84219121283737	36.0	32.0	36.0	21.0	36.0
10	31.71827171400291	36.0	32.0	36.0	14.0	36.0
11	32.98798995347518	36.0	32.0	36.0	21.0	36.0
12	32.3128866993168	36.0	32.0	36.0	21.0	36.0
13	32.66534690495459	36.0	32.0	36.0	21.0	36.0
14	32.25218034737246	36.0	32.0	36.0	21.0	36.0
15	32.132724716836954	36.0	32.0	36.0	14.0	36.0
16	32.28715779427838	36.0	32.0	36.0	21.0	36.0
17	32.02539359098779	36.0	32.0	36.0	14.0	36.0
18	32.22129921297923	36.0	32.0	36.0	14.0	36.0
19	32.1753853693452	36.0	32.0	36.0	14.0	36.0
20	31.949201533416947	36.0	32.0	36.0	14.0	36.0
21	31.966512121280513	36.0	32.0	36.0	14.0	36.0
22	31.9491966971566	36.0	32.0	36.0	14.0	36.0
23	31.860541596675233	36.0	32.0	36.0	14.0	36.0
24	31.859824218057305	36.0	32.0	36.0	14.0	36.0
25	30.968607834096925	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	28.0
5	76.0
6	87.0
7	42.0
8	102.0
9	84.0
10	68.0
11	30.0
12	47.0
13	33.0
14	204.0
15	459.0
16	654.0
17	906.0
18	1256.0
19	1775.0
20	2496.0
21	3579.0
22	4786.0
23	6640.0
24	8977.0
25	12384.0
26	16965.0
27	22409.0
28	29486.0
29	37437.0
30	47956.0
31	61672.0
32	78856.0
33	92837.0
34	119105.0
35	68871.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.47079535872802	18.375973985144963	11.310561199237096	25.842669456889922
2	16.41815347362278	20.244756879454577	38.161727839765014	25.175361807157632
3	18.778051485761452	24.083329034255055	29.133402548493603	28.00521693148989
4	12.497601638141612	15.58846529026225	36.966697032480674	34.94723603911547
5	14.300412020351065	36.41663239882925	34.2531627198181	15.029792861001589
6	33.08523506168857	36.438190468510605	17.152326425288283	13.32424804451254
7	29.11623555222171	30.76535920826578	21.622022729289164	18.49638251022334
8	27.103346574206434	33.459611034562876	20.09202998396252	19.345012407268175
9	26.807901581994564	14.192124235950196	19.524982088800822	39.47499209325442
10	15.46276615489396	26.79229387470526	32.16453549081601	25.58040447958477
11	37.07656627507436	21.403889885040034	22.94357552448568	18.575968315399923
12	24.037927605666543	24.04212456577605	29.65636581995687	22.26358200860054
13	28.63916976686007	19.837149059451082	25.955599312443006	25.568081861245833
14	22.70508789711873	20.536550094578978	25.596525433029694	31.1618365752726
15	24.406271988432067	28.344339653091126	22.56860390805043	24.680784450426373
16	25.023239600278874	26.40502750019366	24.745816871949803	23.82591602757766
17	23.14269020195238	26.551927844636126	26.166972263829347	24.13840968958215
18	24.099843268537004	25.11803283451514	27.412510431277852	23.36961346567
19	24.657476756198346	25.286834969008265	26.095848398760328	23.959839876033058
20	24.77424477918355	25.13254797147132	25.628362118637092	24.464845130708035
21	26.112263427626004	24.601912755740287	25.109882571324803	24.175941245308906
22	25.086411621212783	25.23574510953006	25.764143495759733	23.913699773497424
23	23.634062597359414	24.960491248347413	26.406212922711358	24.99923323158182
24	24.056748441500165	25.563432799682328	26.14437955403176	24.23543920478575
25	24.054512995502986	25.079819602855103	26.262989853467673	24.60267754817424
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	45.0
2	159.5
3	274.0
4	274.0
5	274.0
6	699.5
7	1125.0
8	1125.0
9	1125.0
10	1345.5
11	1566.0
12	1566.0
13	1566.0
14	1898.0
15	2230.0
16	2230.0
17	2230.0
18	3743.5
19	5257.0
20	5257.0
21	5257.0
22	8308.5
23	11360.0
24	11360.0
25	11360.0
26	17009.5
27	22659.0
28	22659.0
29	22659.0
30	28038.5
31	33418.0
32	33418.0
33	33418.0
34	39452.0
35	45486.0
36	45486.0
37	45486.0
38	50301.5
39	55117.0
40	55117.0
41	55117.0
42	60894.0
43	66671.0
44	66671.0
45	66671.0
46	72798.0
47	78925.0
48	78925.0
49	78925.0
50	79066.0
51	79207.0
52	79207.0
53	79207.0
54	72191.0
55	65175.0
56	65175.0
57	65175.0
58	59445.5
59	53716.0
60	53716.0
61	53716.0
62	47272.5
63	40829.0
64	40829.0
65	40829.0
66	33728.0
67	26627.0
68	26627.0
69	26627.0
70	20277.0
71	13927.0
72	13927.0
73	13927.0
74	10793.5
75	7660.0
76	7660.0
77	7660.0
78	6523.0
79	5386.0
80	5386.0
81	5386.0
82	3747.5
83	2109.0
84	2109.0
85	2109.0
86	1538.0
87	967.0
88	967.0
89	967.0
90	629.0
91	291.0
92	291.0
93	291.0
94	165.0
95	39.0
96	39.0
97	39.0
98	143.5
99	248.0
100	248.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008544059943834897
2	0.004030216954639102
3	0.004191425632824666
4	0.015959659140370844
5	0.032080526958927255
6	0.04255909104098892
7	0.06593434937789572
8	0.08318367794375107
9	0.09511312012948281
10	0.11332970076445156
11	0.11397453547719381
12	0.13186869875579144
13	0.11752112639727623
14	0.11703750036271954
15	0.10800981438432794
16	0.10994431852255471
17	0.12284101277739982
18	0.1262263950192967
19	0.1279996904793379
20	0.11719870904090508
21	0.12719364708841005
22	0.14428176697607986
23	0.13396441157220376
24	0.1302566119739358
25	0.12767727312296676
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	620314.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.22904187304937
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.54615178509295	51.523627579426
2	9.483390387930509	11.423510335462812
3	2.165504048120181	3.912787019714649
4	0.8287539999176512	1.9966023745398946
5	0.4409835829091048	1.3280009340179904
6	0.2763720960192156	0.9987375926210253
7	0.1776276941363792	0.7488842079567232
8	0.12782226060520546	0.6158889829038998
9	0.08347250230248371	0.4524721952782051
>10	0.6528954408340789	8.02684053869463
>50	0.11051520105700209	4.733958164046265
>100	0.09957043458919648	11.459315008069733
>500	0.006673621621258155	2.576633587553673
>1k	2.669448648503262E-4	0.20274147971453774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1261	0.2032841431919963	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	970	0.15637241783999717	No Hit
TATCAACGCAGAGTACTTTTTTTTT	907	0.14621627111430663	No Hit
GAACTACGACGGTATCTGATCGTCT	779	0.12558156030655443	No Hit
GTATCTGATCGTCTTCGAACCTCCG	751	0.12106771731735862	No Hit
GAATAGGACCGCGGTTCTATTTTGT	737	0.11881079582276075	No Hit
GAATAACGCCGCCGCATCGCCAGTC	724	0.11671508300634839	No Hit
ATCAGATACCGTCGTAGTTCCGACC	711	0.11461937018993607	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	698	0.11252365737352372	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	694	0.11187882266078146	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	689	0.11107277926985365	No Hit
GTCCTATTCCATTATTCCTAGCTGC	639	0.10301234536057545	No Hit
TCGTAGTTCCGACCATAAACGATGC	636	0.10252871932601876	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	634	0.10220630196964764	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.6120867818556408E-4	0.0
8	0.0	0.0	0.0	1.6120867818556408E-4	0.0
9	0.0	0.0	0.0	1.6120867818556408E-4	0.0
10	0.0	0.0	0.0	1.6120867818556408E-4	0.0
11	0.0	0.0	0.0	1.6120867818556408E-4	0.0
12	0.0	0.0	0.0	1.6120867818556408E-4	0.0
13	0.0	0.0	0.0	1.6120867818556408E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	220	0.0	16.398016	1
GTCTTAG	60	1.4736779E-6	15.822647	1
CGTTTCC	70	4.4209264E-7	14.929333	14
CGGTCCA	155	0.0	14.711615	10
TGAACGG	40	0.005275403	14.249577	5
CCTAAAT	40	0.0052895444	14.243829	2
GTATCAA	800	0.0	13.884374	1
TATACTG	55	1.9576536E-4	13.817771	5
CTTAGAC	55	1.963022E-4	13.813312	3
GTACTGG	70	7.2964176E-6	13.562269	1
GCATCGC	110	2.730303E-9	12.955206	14
CGCATCG	105	1.9832441E-8	12.667314	13
GAACCGC	75	1.47441515E-5	12.667312	6
AGAACCG	60	4.0868818E-4	12.66629	5
TTAGGAC	120	7.403287E-10	12.662203	3
TCGCCAG	115	5.2732503E-9	12.394937	17
CCGACCA	115	5.285983E-9	12.392936	9
TAACTTT	85	3.9302704E-6	12.2947445	6
CCAACGA	70	1.08670225E-4	12.216881	19
GATGCAA	70	1.0875216E-4	12.215895	16
>>END_MODULE