FastQCFastQC Report
Thu 2 Feb 2017
SRR4062631_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062631_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620314
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9960.16056384347282182No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG9520.15347066163265702No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8730.14073517605599745No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGAT7920.12767727312296676No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7540.12155134335191532No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7340.11832716978820404No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC6790.109460692487998No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6760.10897706645344132No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA6420.10349597139513214No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC6380.10285113668238989No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAC250.002349187235.2083171
CGCGGGA602.8707014E-421.9998844
TACCCCG500.002579117721.9998785
GGTCGTA803.6110196E-621.99987829
TTGTCGA703.2122356E-521.99987844
GTATCAA9100.021.5215681
ATTGTCG755.467364E-520.5332243
GTCCTAA2150.020.4699541
TTGTACT1401.8371793E-1020.428464
GTATTGA550.00447506820.0047281
GTTATAT1101.2599958E-720.0047281
CGGTCCA2250.019.55544710
TAGGACG2150.019.4417534
CGCCGGT2300.019.130337
GTGTATA2550.018.984881
GCCGGTC2550.018.980298
GTAGAAC3600.018.944343
AACGCAG10250.018.8877036
CGTATAT1404.0708983E-918.85703810
TAGACCA1404.0708983E-918.8570384
Produced by FastQC (version 0.11.5)